Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3631 and Pf6N2E2_3632 are separated by 392 nucleotides Pf6N2E2_3632 and Pf6N2E2_3633 are separated by 242 nucleotides
Pf6N2E2_3631 - HAMP domain protein, at 4,228,393 to 4,229,907
_3631
Pf6N2E2_3632 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,230,300 to 4,230,941
_3632
Pf6N2E2_3633 - Topoisomerase IV subunit A (EC 5.99.1.-), at 4,231,184 to 4,233,448
_3633
Position (kb)
4230
4231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4230.212 kb on + strand at 4230.283 kb on + strand at 4230.283 kb on + strand at 4230.283 kb on + strand at 4230.284 kb on - strand at 4230.284 kb on - strand at 4230.284 kb on - strand at 4230.284 kb on - strand at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.416 kb on + strand, within Pf6N2E2_3632 at 4230.417 kb on - strand, within Pf6N2E2_3632 at 4230.614 kb on + strand, within Pf6N2E2_3632 at 4230.615 kb on - strand, within Pf6N2E2_3632 at 4230.651 kb on + strand, within Pf6N2E2_3632 at 4230.651 kb on + strand, within Pf6N2E2_3632 at 4230.652 kb on - strand, within Pf6N2E2_3632 at 4230.652 kb on - strand, within Pf6N2E2_3632 at 4230.652 kb on - strand, within Pf6N2E2_3632 at 4230.652 kb on - strand, within Pf6N2E2_3632 at 4230.816 kb on + strand, within Pf6N2E2_3632 at 4230.816 kb on + strand, within Pf6N2E2_3632 at 4230.836 kb on - strand, within Pf6N2E2_3632 at 4230.864 kb on + strand, within Pf6N2E2_3632 at 4230.865 kb on - strand, within Pf6N2E2_3632 at 4230.919 kb on + strand at 4230.920 kb on - strand at 4230.920 kb on - strand at 4230.984 kb on - strand at 4230.984 kb on - strand at 4231.034 kb on + strand at 4231.034 kb on + strand at 4231.035 kb on - strand at 4231.035 kb on - strand at 4231.062 kb on - strand at 4231.100 kb on + strand at 4231.100 kb on + strand at 4231.100 kb on + strand at 4231.101 kb on - strand at 4231.157 kb on - strand at 4231.157 kb on - strand at 4231.177 kb on - strand at 4231.177 kb on - strand at 4231.177 kb on - strand at 4231.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,230,212 + -1.6 4,230,283 + -0.3 4,230,283 + -2.8 4,230,283 + -0.8 4,230,284 - +0.7 4,230,284 - -0.5 4,230,284 - +0.4 4,230,284 - -1.1 4,230,416 + Pf6N2E2_3632 0.18 -2.1 4,230,416 + Pf6N2E2_3632 0.18 -0.9 4,230,416 + Pf6N2E2_3632 0.18 -1.3 4,230,416 + Pf6N2E2_3632 0.18 -3.5 4,230,416 + Pf6N2E2_3632 0.18 -0.9 4,230,416 + Pf6N2E2_3632 0.18 -3.2 4,230,417 - Pf6N2E2_3632 0.18 -3.3 4,230,614 + Pf6N2E2_3632 0.49 -1.5 4,230,615 - Pf6N2E2_3632 0.49 -3.6 4,230,651 + Pf6N2E2_3632 0.55 -2.2 4,230,651 + Pf6N2E2_3632 0.55 -2.0 4,230,652 - Pf6N2E2_3632 0.55 -3.2 4,230,652 - Pf6N2E2_3632 0.55 -3.5 4,230,652 - Pf6N2E2_3632 0.55 -0.8 4,230,652 - Pf6N2E2_3632 0.55 -0.8 4,230,816 + Pf6N2E2_3632 0.80 -3.6 4,230,816 + Pf6N2E2_3632 0.80 -1.0 4,230,836 - Pf6N2E2_3632 0.83 -3.0 4,230,864 + Pf6N2E2_3632 0.88 -3.2 4,230,865 - Pf6N2E2_3632 0.88 -2.2 4,230,919 + -3.5 4,230,920 - -3.0 4,230,920 - -2.9 4,230,984 - -2.7 4,230,984 - -1.9 4,231,034 + -1.3 4,231,034 + +0.1 4,231,035 - -2.9 4,231,035 - -1.7 4,231,062 - +2.1 4,231,100 + -2.3 4,231,100 + -1.3 4,231,100 + -3.5 4,231,101 - +0.8 4,231,157 - +2.2 4,231,157 - +2.0 4,231,177 - +0.7 4,231,177 - +2.0 4,231,177 - +2.8 4,231,186 - +0.7
Or see this region's nucleotide sequence