Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3623

Experiment: Phosphomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3621 and Pf6N2E2_3622 are separated by 264 nucleotidesPf6N2E2_3622 and Pf6N2E2_3623 are separated by 109 nucleotidesPf6N2E2_3623 and Pf6N2E2_3624 are separated by 123 nucleotides Pf6N2E2_3621 - putative transporter, required for glycine utilization (from data), at 4,216,901 to 4,217,512 _3621 Pf6N2E2_3622 - 3'-to-5' oligoribonuclease (orn), at 4,217,777 to 4,218,319 _3622 Pf6N2E2_3623 - Ribosome small subunit-stimulated GTPase EngC, at 4,218,429 to 4,219,460 _3623 Pf6N2E2_3624 - Flagellar motor rotation protein MotB, at 4,219,584 to 4,220,621 _3624 Position (kb) 4218 4219 4220Strain fitness (log2 ratio) -2 -1 0 1at 4217.496 kb on + strandat 4217.496 kb on + strandat 4217.496 kb on + strandat 4217.497 kb on - strandat 4217.598 kb on + strandat 4217.598 kb on + strandat 4217.598 kb on + strandat 4217.599 kb on - strandat 4217.599 kb on - strandat 4218.338 kb on - strandat 4218.338 kb on - strandat 4218.350 kb on + strandat 4218.351 kb on - strandat 4218.351 kb on - strandat 4218.355 kb on + strandat 4218.356 kb on - strandat 4218.356 kb on - strandat 4218.394 kb on - strandat 4218.398 kb on + strandat 4218.398 kb on + strandat 4218.417 kb on + strandat 4219.455 kb on + strandat 4219.456 kb on - strandat 4219.579 kb on - strandat 4219.829 kb on + strand, within Pf6N2E2_3624at 4219.830 kb on - strand, within Pf6N2E2_3624at 4220.002 kb on + strand, within Pf6N2E2_3624at 4220.018 kb on + strand, within Pf6N2E2_3624at 4220.092 kb on + strand, within Pf6N2E2_3624at 4220.093 kb on - strand, within Pf6N2E2_3624at 4220.404 kb on + strand, within Pf6N2E2_3624at 4220.404 kb on + strand, within Pf6N2E2_3624at 4220.405 kb on - strand, within Pf6N2E2_3624at 4220.405 kb on - strand, within Pf6N2E2_3624at 4220.405 kb on - strand, within Pf6N2E2_3624at 4220.405 kb on - strand, within Pf6N2E2_3624at 4220.446 kb on + strand, within Pf6N2E2_3624at 4220.447 kb on - strand, within Pf6N2E2_3624at 4220.447 kb on - strand, within Pf6N2E2_3624

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml
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4,217,496 + -1.2
4,217,496 + -1.7
4,217,496 + -0.8
4,217,497 - -1.5
4,217,598 + +1.2
4,217,598 + -0.0
4,217,598 + +0.4
4,217,599 - -1.6
4,217,599 - -0.2
4,218,338 - +0.8
4,218,338 - +0.1
4,218,350 + -0.2
4,218,351 - +1.1
4,218,351 - +0.7
4,218,355 + -2.1
4,218,356 - -0.1
4,218,356 - -0.2
4,218,394 - -0.9
4,218,398 + +0.2
4,218,398 + -0.7
4,218,417 + -0.6
4,219,455 + -0.2
4,219,456 - -0.2
4,219,579 - -0.2
4,219,829 + Pf6N2E2_3624 0.24 -1.4
4,219,830 - Pf6N2E2_3624 0.24 +0.1
4,220,002 + Pf6N2E2_3624 0.40 -0.1
4,220,018 + Pf6N2E2_3624 0.42 -1.2
4,220,092 + Pf6N2E2_3624 0.49 +0.2
4,220,093 - Pf6N2E2_3624 0.49 +0.9
4,220,404 + Pf6N2E2_3624 0.79 -0.2
4,220,404 + Pf6N2E2_3624 0.79 +1.0
4,220,405 - Pf6N2E2_3624 0.79 -0.1
4,220,405 - Pf6N2E2_3624 0.79 +1.5
4,220,405 - Pf6N2E2_3624 0.79 +0.1
4,220,405 - Pf6N2E2_3624 0.79 -0.3
4,220,446 + Pf6N2E2_3624 0.83 -2.3
4,220,447 - Pf6N2E2_3624 0.83 -0.2
4,220,447 - Pf6N2E2_3624 0.83 -1.6

Or see this region's nucleotide sequence