Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3616 and Pf6N2E2_3617 overlap by 4 nucleotides Pf6N2E2_3617 and Pf6N2E2_3618 are separated by 3 nucleotides Pf6N2E2_3618 and Pf6N2E2_3619 overlap by 13 nucleotides
Pf6N2E2_3616 - DNA mismatch repair protein MutL, at 4,210,481 to 4,212,394
_3616
Pf6N2E2_3617 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,212,391 to 4,213,818
_3617
Pf6N2E2_3618 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 4,213,822 to 4,214,289
_3618
Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776
_3619
Position (kb)
4212
4213
4214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4211.404 kb on - strand, within Pf6N2E2_3616 at 4211.442 kb on - strand, within Pf6N2E2_3616 at 4211.442 kb on - strand, within Pf6N2E2_3616 at 4211.442 kb on - strand, within Pf6N2E2_3616 at 4211.463 kb on - strand, within Pf6N2E2_3616 at 4211.463 kb on - strand, within Pf6N2E2_3616 at 4211.463 kb on - strand, within Pf6N2E2_3616 at 4211.463 kb on - strand, within Pf6N2E2_3616 at 4211.463 kb on - strand, within Pf6N2E2_3616 at 4211.601 kb on + strand, within Pf6N2E2_3616 at 4211.602 kb on - strand, within Pf6N2E2_3616 at 4211.602 kb on - strand, within Pf6N2E2_3616 at 4211.608 kb on - strand, within Pf6N2E2_3616 at 4212.038 kb on - strand, within Pf6N2E2_3616 at 4212.596 kb on + strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4212.597 kb on - strand, within Pf6N2E2_3617 at 4213.804 kb on - strand at 4213.824 kb on - strand at 4214.271 kb on - strand at 4214.578 kb on + strand, within Pf6N2E2_3619 at 4214.579 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,211,404 - Pf6N2E2_3616 0.48 -3.5 4,211,442 - Pf6N2E2_3616 0.50 -2.6 4,211,442 - Pf6N2E2_3616 0.50 -1.1 4,211,442 - Pf6N2E2_3616 0.50 -2.3 4,211,463 - Pf6N2E2_3616 0.51 -0.0 4,211,463 - Pf6N2E2_3616 0.51 +1.8 4,211,463 - Pf6N2E2_3616 0.51 -1.6 4,211,463 - Pf6N2E2_3616 0.51 +0.1 4,211,463 - Pf6N2E2_3616 0.51 -2.0 4,211,601 + Pf6N2E2_3616 0.59 -1.1 4,211,602 - Pf6N2E2_3616 0.59 -1.1 4,211,602 - Pf6N2E2_3616 0.59 +0.1 4,211,608 - Pf6N2E2_3616 0.59 -2.4 4,212,038 - Pf6N2E2_3616 0.81 -1.0 4,212,596 + Pf6N2E2_3617 0.14 -0.3 4,212,597 - Pf6N2E2_3617 0.14 -0.6 4,212,597 - Pf6N2E2_3617 0.14 -1.0 4,212,597 - Pf6N2E2_3617 0.14 -2.0 4,212,597 - Pf6N2E2_3617 0.14 +0.5 4,212,597 - Pf6N2E2_3617 0.14 -2.1 4,212,597 - Pf6N2E2_3617 0.14 -0.2 4,212,597 - Pf6N2E2_3617 0.14 -0.9 4,212,597 - Pf6N2E2_3617 0.14 -0.4 4,212,597 - Pf6N2E2_3617 0.14 -1.3 4,212,597 - Pf6N2E2_3617 0.14 -2.2 4,212,597 - Pf6N2E2_3617 0.14 -2.5 4,213,804 - -2.5 4,213,824 - -0.3 4,214,271 - +1.5 4,214,578 + Pf6N2E2_3619 0.20 +0.5 4,214,579 - Pf6N2E2_3619 0.20 +1.9 4,214,743 - Pf6N2E2_3619 0.31 -0.6 4,214,743 - Pf6N2E2_3619 0.31 +0.7 4,214,743 - Pf6N2E2_3619 0.31 +1.0 4,214,743 - Pf6N2E2_3619 0.31 -0.1 4,214,743 - Pf6N2E2_3619 0.31 +1.3
Or see this region's nucleotide sequence