Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3504 and Pf6N2E2_3505 are separated by 211 nucleotides Pf6N2E2_3505 and Pf6N2E2_3506 are separated by 168 nucleotides Pf6N2E2_3506 and Pf6N2E2_3507 are separated by 36 nucleotides
Pf6N2E2_3504 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 4,083,886 to 4,085,799
_3504
Pf6N2E2_3505 - Translation initiation factor SUI1-related protein, at 4,086,011 to 4,086,382
_3505
Pf6N2E2_3506 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 4,086,551 to 4,087,075
_3506
Pf6N2E2_3507 - FIG014328: hypothetical protein, at 4,087,112 to 4,088,179
_3507
Position (kb)
4086
4087 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4085.331 kb on - strand, within Pf6N2E2_3504 at 4085.331 kb on - strand, within Pf6N2E2_3504 at 4085.492 kb on + strand, within Pf6N2E2_3504 at 4085.492 kb on + strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.493 kb on - strand, within Pf6N2E2_3504 at 4085.505 kb on - strand, within Pf6N2E2_3504 at 4085.811 kb on - strand at 4085.812 kb on + strand at 4085.812 kb on + strand at 4085.813 kb on - strand at 4085.813 kb on - strand at 4085.856 kb on + strand at 4085.857 kb on - strand at 4085.857 kb on - strand at 4085.892 kb on + strand at 4085.892 kb on + strand at 4085.893 kb on - strand at 4085.918 kb on - strand at 4085.918 kb on - strand at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.552 kb on + strand at 4086.552 kb on + strand at 4086.553 kb on - strand at 4086.553 kb on - strand at 4086.554 kb on + strand at 4086.555 kb on - strand at 4086.555 kb on - strand at 4086.591 kb on + strand at 4086.591 kb on + strand at 4086.592 kb on - strand at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.625 kb on - strand, within Pf6N2E2_3506 at 4086.768 kb on + strand, within Pf6N2E2_3506 at 4086.846 kb on + strand, within Pf6N2E2_3506 at 4086.847 kb on - strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.901 kb on - strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4087.170 kb on + strand at 4087.170 kb on + strand at 4087.202 kb on + strand at 4087.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 4,085,331 - Pf6N2E2_3504 0.75 -2.8 4,085,331 - Pf6N2E2_3504 0.75 -0.1 4,085,492 + Pf6N2E2_3504 0.84 -1.5 4,085,492 + Pf6N2E2_3504 0.84 -1.7 4,085,493 - Pf6N2E2_3504 0.84 -0.6 4,085,493 - Pf6N2E2_3504 0.84 -1.2 4,085,493 - Pf6N2E2_3504 0.84 -3.3 4,085,505 - Pf6N2E2_3504 0.85 -1.5 4,085,811 - -1.1 4,085,812 + +0.3 4,085,812 + -1.0 4,085,813 - -1.7 4,085,813 - -0.2 4,085,856 + +1.3 4,085,857 - +1.0 4,085,857 - -1.0 4,085,892 + -3.5 4,085,892 + -0.4 4,085,893 - +0.5 4,085,918 - +0.4 4,085,918 - +2.2 4,086,338 + Pf6N2E2_3505 0.88 -1.8 4,086,338 + Pf6N2E2_3505 0.88 -0.9 4,086,552 + -0.6 4,086,552 + -1.8 4,086,553 - +1.4 4,086,553 - +0.6 4,086,554 + -1.1 4,086,555 - +0.0 4,086,555 - +1.3 4,086,591 + +0.0 4,086,591 + -1.4 4,086,592 - -0.1 4,086,624 + Pf6N2E2_3506 0.14 +0.0 4,086,624 + Pf6N2E2_3506 0.14 -1.8 4,086,625 - Pf6N2E2_3506 0.14 +1.2 4,086,768 + Pf6N2E2_3506 0.41 -0.5 4,086,846 + Pf6N2E2_3506 0.56 -0.4 4,086,847 - Pf6N2E2_3506 0.56 -0.4 4,086,900 + Pf6N2E2_3506 0.66 -0.2 4,086,900 + Pf6N2E2_3506 0.66 -1.0 4,086,901 - Pf6N2E2_3506 0.67 -1.4 4,086,966 + Pf6N2E2_3506 0.79 -0.2 4,086,966 + Pf6N2E2_3506 0.79 -0.0 4,086,966 + Pf6N2E2_3506 0.79 +0.5 4,086,966 + Pf6N2E2_3506 0.79 -0.9 4,086,966 + Pf6N2E2_3506 0.79 -2.1 4,086,967 - Pf6N2E2_3506 0.79 +0.3 4,086,967 - Pf6N2E2_3506 0.79 +1.1 4,086,967 - Pf6N2E2_3506 0.79 -0.6 4,086,967 - Pf6N2E2_3506 0.79 +0.2 4,086,967 - Pf6N2E2_3506 0.79 +1.1 4,086,967 - Pf6N2E2_3506 0.79 +1.1 4,086,967 - Pf6N2E2_3506 0.79 -0.7 4,086,967 - Pf6N2E2_3506 0.79 -0.2 4,086,967 - Pf6N2E2_3506 0.79 +0.8 4,087,170 + -0.0 4,087,170 + -0.2 4,087,202 + -0.4 4,087,203 - +0.1
Or see this region's nucleotide sequence