Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3349 and Pf6N2E2_3350 are separated by 194 nucleotides Pf6N2E2_3350 and Pf6N2E2_3351 are separated by 143 nucleotides Pf6N2E2_3351 and Pf6N2E2_3352 are separated by 4 nucleotides Pf6N2E2_3352 and Pf6N2E2_3353 are separated by 159 nucleotides
Pf6N2E2_3349 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 3,877,057 to 3,879,162
_3349
Pf6N2E2_3350 - SSU ribosomal protein S15p (S13e), at 3,879,357 to 3,879,626
_3350
Pf6N2E2_3351 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 3,879,770 to 3,880,687
_3351
Pf6N2E2_3352 - Ribosome-binding factor A, at 3,880,692 to 3,881,093
_3352
Pf6N2E2_3353 - Translation initiation factor 2, at 3,881,253 to 3,883,772
_3353
Position (kb)
3879
3880
3881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3879.272 kb on - strand at 3879.272 kb on - strand at 3879.848 kb on - strand at 3879.897 kb on - strand, within Pf6N2E2_3351 at 3879.919 kb on - strand, within Pf6N2E2_3351 at 3880.102 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.190 kb on - strand, within Pf6N2E2_3351 at 3880.193 kb on - strand, within Pf6N2E2_3351 at 3880.229 kb on - strand, within Pf6N2E2_3351 at 3880.253 kb on + strand, within Pf6N2E2_3351 at 3880.276 kb on + strand, within Pf6N2E2_3351 at 3880.276 kb on + strand, within Pf6N2E2_3351 at 3880.277 kb on - strand, within Pf6N2E2_3351 at 3880.278 kb on + strand, within Pf6N2E2_3351 at 3880.311 kb on + strand, within Pf6N2E2_3351
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,879,272 - +1.3 3,879,272 - +1.2 3,879,848 - -0.9 3,879,897 - Pf6N2E2_3351 0.14 -2.6 3,879,919 - Pf6N2E2_3351 0.16 -3.1 3,880,102 - Pf6N2E2_3351 0.36 -1.6 3,880,153 - Pf6N2E2_3351 0.42 -0.5 3,880,153 - Pf6N2E2_3351 0.42 -1.3 3,880,153 - Pf6N2E2_3351 0.42 -1.6 3,880,153 - Pf6N2E2_3351 0.42 -3.1 3,880,190 - Pf6N2E2_3351 0.46 -2.4 3,880,193 - Pf6N2E2_3351 0.46 -1.9 3,880,229 - Pf6N2E2_3351 0.50 -2.2 3,880,253 + Pf6N2E2_3351 0.53 -2.9 3,880,276 + Pf6N2E2_3351 0.55 -2.5 3,880,276 + Pf6N2E2_3351 0.55 -0.3 3,880,277 - Pf6N2E2_3351 0.55 -0.6 3,880,278 + Pf6N2E2_3351 0.55 -1.3 3,880,311 + Pf6N2E2_3351 0.59 -3.0
Or see this region's nucleotide sequence