Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3345 and Pf6N2E2_3346 are separated by 226 nucleotides Pf6N2E2_3346 and Pf6N2E2_3347 are separated by 98 nucleotides Pf6N2E2_3347 and Pf6N2E2_3348 are separated by 305 nucleotides
Pf6N2E2_3345 - Thymidine phosphorylase (EC 2.4.2.4), at 3,872,299 to 3,874,512
_3345
Pf6N2E2_3346 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 3,874,739 to 3,875,587
_3346
Pf6N2E2_3347 - FIG00957702: hypothetical protein, at 3,875,686 to 3,875,991
_3347
Pf6N2E2_3348 - hypothetical protein, at 3,876,297 to 3,876,845
_3348
Position (kb)
3874
3875
3876 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3873.760 kb on + strand, within Pf6N2E2_3345 at 3873.760 kb on + strand, within Pf6N2E2_3345 at 3873.761 kb on - strand, within Pf6N2E2_3345 at 3873.761 kb on - strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.875 kb on + strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.876 kb on - strand, within Pf6N2E2_3345 at 3873.902 kb on + strand, within Pf6N2E2_3345 at 3873.902 kb on + strand, within Pf6N2E2_3345 at 3873.903 kb on - strand, within Pf6N2E2_3345 at 3873.903 kb on - strand, within Pf6N2E2_3345 at 3873.903 kb on - strand, within Pf6N2E2_3345 at 3873.952 kb on + strand, within Pf6N2E2_3345 at 3873.952 kb on + strand, within Pf6N2E2_3345 at 3873.952 kb on + strand, within Pf6N2E2_3345 at 3873.952 kb on + strand, within Pf6N2E2_3345 at 3873.953 kb on - strand, within Pf6N2E2_3345 at 3874.150 kb on + strand, within Pf6N2E2_3345 at 3874.150 kb on + strand, within Pf6N2E2_3345 at 3874.150 kb on + strand, within Pf6N2E2_3345 at 3874.151 kb on - strand, within Pf6N2E2_3345 at 3874.151 kb on - strand, within Pf6N2E2_3345 at 3874.263 kb on + strand, within Pf6N2E2_3345 at 3874.263 kb on + strand, within Pf6N2E2_3345 at 3874.264 kb on - strand, within Pf6N2E2_3345 at 3874.264 kb on - strand, within Pf6N2E2_3345 at 3874.264 kb on - strand, within Pf6N2E2_3345 at 3874.298 kb on + strand at 3874.492 kb on + strand at 3874.492 kb on + strand at 3874.492 kb on + strand at 3874.492 kb on + strand at 3874.492 kb on + strand at 3874.492 kb on + strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.493 kb on - strand at 3874.557 kb on + strand at 3874.557 kb on + strand at 3874.557 kb on + strand at 3874.557 kb on + strand at 3874.558 kb on - strand at 3874.558 kb on - strand at 3874.605 kb on - strand at 3874.644 kb on + strand at 3874.644 kb on + strand at 3874.645 kb on - strand at 3874.645 kb on - strand at 3874.661 kb on - strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.706 kb on + strand at 3874.707 kb on - strand at 3874.707 kb on - strand at 3874.707 kb on - strand at 3874.707 kb on - strand at 3874.707 kb on - strand at 3874.707 kb on - strand at 3874.708 kb on + strand at 3874.709 kb on - strand at 3874.749 kb on + strand at 3874.749 kb on + strand at 3874.954 kb on - strand, within Pf6N2E2_3346 at 3875.183 kb on + strand, within Pf6N2E2_3346 at 3875.183 kb on + strand, within Pf6N2E2_3346 at 3875.183 kb on + strand, within Pf6N2E2_3346 at 3875.184 kb on - strand, within Pf6N2E2_3346 at 3875.184 kb on - strand, within Pf6N2E2_3346 at 3875.184 kb on - strand, within Pf6N2E2_3346 at 3875.184 kb on - strand, within Pf6N2E2_3346 at 3875.184 kb on - strand, within Pf6N2E2_3346 at 3875.228 kb on + strand, within Pf6N2E2_3346 at 3875.229 kb on - strand, within Pf6N2E2_3346 at 3875.229 kb on - strand, within Pf6N2E2_3346 at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.519 kb on + strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.520 kb on - strand at 3875.581 kb on + strand at 3875.687 kb on + strand at 3875.687 kb on + strand at 3875.688 kb on - strand at 3875.688 kb on - strand at 3875.688 kb on - strand at 3875.688 kb on - strand at 3875.688 kb on - strand at 3875.692 kb on + strand at 3875.701 kb on - strand at 3875.701 kb on - strand at 3875.701 kb on - strand at 3875.714 kb on + strand at 3875.715 kb on - strand at 3875.801 kb on + strand, within Pf6N2E2_3347 at 3875.801 kb on + strand, within Pf6N2E2_3347 at 3875.802 kb on - strand, within Pf6N2E2_3347 at 3875.802 kb on - strand, within Pf6N2E2_3347 at 3875.932 kb on + strand, within Pf6N2E2_3347 at 3875.932 kb on + strand, within Pf6N2E2_3347 at 3875.932 kb on + strand, within Pf6N2E2_3347 at 3875.933 kb on - strand, within Pf6N2E2_3347 at 3875.933 kb on - strand, within Pf6N2E2_3347 at 3875.933 kb on - strand, within Pf6N2E2_3347 at 3875.936 kb on + strand, within Pf6N2E2_3347 at 3875.936 kb on + strand, within Pf6N2E2_3347 at 3875.937 kb on - strand, within Pf6N2E2_3347 at 3876.077 kb on + strand at 3876.078 kb on - strand at 3876.145 kb on + strand at 3876.145 kb on + strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.147 kb on + strand at 3876.147 kb on + strand at 3876.148 kb on - strand at 3876.148 kb on - strand at 3876.169 kb on - strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.209 kb on - strand at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.429 kb on - strand, within Pf6N2E2_3348 at 3876.445 kb on - strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,873,760 + Pf6N2E2_3345 0.66 -1.5 3,873,760 + Pf6N2E2_3345 0.66 +0.0 3,873,761 - Pf6N2E2_3345 0.66 -1.0 3,873,761 - Pf6N2E2_3345 0.66 +0.5 3,873,875 + Pf6N2E2_3345 0.71 +1.1 3,873,875 + Pf6N2E2_3345 0.71 +0.6 3,873,875 + Pf6N2E2_3345 0.71 +0.4 3,873,875 + Pf6N2E2_3345 0.71 -0.1 3,873,875 + Pf6N2E2_3345 0.71 +1.4 3,873,875 + Pf6N2E2_3345 0.71 +0.8 3,873,875 + Pf6N2E2_3345 0.71 -0.1 3,873,876 - Pf6N2E2_3345 0.71 +0.2 3,873,876 - Pf6N2E2_3345 0.71 +1.2 3,873,876 - Pf6N2E2_3345 0.71 -0.4 3,873,876 - Pf6N2E2_3345 0.71 +0.1 3,873,876 - Pf6N2E2_3345 0.71 +0.6 3,873,876 - Pf6N2E2_3345 0.71 -0.7 3,873,902 + Pf6N2E2_3345 0.72 +0.4 3,873,902 + Pf6N2E2_3345 0.72 +0.6 3,873,903 - Pf6N2E2_3345 0.72 -0.1 3,873,903 - Pf6N2E2_3345 0.72 +0.1 3,873,903 - Pf6N2E2_3345 0.72 +0.6 3,873,952 + Pf6N2E2_3345 0.75 -1.6 3,873,952 + Pf6N2E2_3345 0.75 +0.5 3,873,952 + Pf6N2E2_3345 0.75 -2.0 3,873,952 + Pf6N2E2_3345 0.75 +0.9 3,873,953 - Pf6N2E2_3345 0.75 -1.5 3,874,150 + Pf6N2E2_3345 0.84 -0.5 3,874,150 + Pf6N2E2_3345 0.84 +0.2 3,874,150 + Pf6N2E2_3345 0.84 +0.8 3,874,151 - Pf6N2E2_3345 0.84 +0.1 3,874,151 - Pf6N2E2_3345 0.84 +0.2 3,874,263 + Pf6N2E2_3345 0.89 -0.2 3,874,263 + Pf6N2E2_3345 0.89 -0.3 3,874,264 - Pf6N2E2_3345 0.89 +1.3 3,874,264 - Pf6N2E2_3345 0.89 -1.3 3,874,264 - Pf6N2E2_3345 0.89 -0.3 3,874,298 + +1.6 3,874,492 + -1.2 3,874,492 + -0.1 3,874,492 + -1.1 3,874,492 + -0.9 3,874,492 + -0.5 3,874,492 + -0.2 3,874,493 - -1.4 3,874,493 - -0.1 3,874,493 - -1.4 3,874,493 - -0.8 3,874,493 - -1.0 3,874,493 - -0.6 3,874,493 - +0.7 3,874,557 + +0.8 3,874,557 + +0.3 3,874,557 + +1.8 3,874,557 + +0.9 3,874,558 - +0.3 3,874,558 - -0.2 3,874,605 - -2.1 3,874,644 + -0.0 3,874,644 + -0.1 3,874,645 - -1.9 3,874,645 - -1.5 3,874,661 - -0.6 3,874,706 + -0.3 3,874,706 + -1.0 3,874,706 + +0.0 3,874,706 + -0.4 3,874,706 + +0.9 3,874,706 + +0.3 3,874,706 + -1.6 3,874,706 + +0.4 3,874,707 - +0.5 3,874,707 - -0.2 3,874,707 - -0.3 3,874,707 - +0.2 3,874,707 - +0.7 3,874,707 - -1.0 3,874,708 + -0.2 3,874,709 - -2.4 3,874,749 + -2.7 3,874,749 + -3.0 3,874,954 - Pf6N2E2_3346 0.25 -3.3 3,875,183 + Pf6N2E2_3346 0.52 -0.6 3,875,183 + Pf6N2E2_3346 0.52 -2.9 3,875,183 + Pf6N2E2_3346 0.52 -1.8 3,875,184 - Pf6N2E2_3346 0.52 -3.4 3,875,184 - Pf6N2E2_3346 0.52 -1.1 3,875,184 - Pf6N2E2_3346 0.52 -2.7 3,875,184 - Pf6N2E2_3346 0.52 -3.6 3,875,184 - Pf6N2E2_3346 0.52 -2.7 3,875,228 + Pf6N2E2_3346 0.58 -4.3 3,875,229 - Pf6N2E2_3346 0.58 -2.5 3,875,229 - Pf6N2E2_3346 0.58 -2.0 3,875,519 + -1.8 3,875,519 + -1.9 3,875,519 + -2.7 3,875,519 + -3.6 3,875,519 + -2.2 3,875,519 + -2.4 3,875,519 + -1.9 3,875,520 - -3.5 3,875,520 - -0.3 3,875,520 - -2.8 3,875,520 - -1.2 3,875,520 - -3.1 3,875,520 - -3.3 3,875,520 - -3.5 3,875,520 - -3.7 3,875,520 - -3.7 3,875,581 + +0.3 3,875,687 + +0.6 3,875,687 + +1.8 3,875,688 - +0.4 3,875,688 - +0.6 3,875,688 - -0.2 3,875,688 - -0.2 3,875,688 - -0.5 3,875,692 + -1.0 3,875,701 - +1.5 3,875,701 - -1.5 3,875,701 - -1.6 3,875,714 + +0.7 3,875,715 - -1.0 3,875,801 + Pf6N2E2_3347 0.38 -0.6 3,875,801 + Pf6N2E2_3347 0.38 +1.2 3,875,802 - Pf6N2E2_3347 0.38 -0.7 3,875,802 - Pf6N2E2_3347 0.38 -0.1 3,875,932 + Pf6N2E2_3347 0.80 -0.0 3,875,932 + Pf6N2E2_3347 0.80 +0.3 3,875,932 + Pf6N2E2_3347 0.80 +0.4 3,875,933 - Pf6N2E2_3347 0.81 -0.8 3,875,933 - Pf6N2E2_3347 0.81 -0.5 3,875,933 - Pf6N2E2_3347 0.81 -0.1 3,875,936 + Pf6N2E2_3347 0.82 -0.4 3,875,936 + Pf6N2E2_3347 0.82 +1.2 3,875,937 - Pf6N2E2_3347 0.82 -1.2 3,876,077 + +0.3 3,876,078 - +0.9 3,876,145 + +0.8 3,876,145 + +0.6 3,876,146 - -0.8 3,876,146 - +0.1 3,876,146 - -0.4 3,876,146 - -0.2 3,876,146 - -0.3 3,876,146 - +0.2 3,876,147 + +0.7 3,876,147 + +1.8 3,876,148 - +0.7 3,876,148 - -1.7 3,876,169 - -2.5 3,876,180 + -0.1 3,876,180 + -0.1 3,876,180 + +0.9 3,876,180 + +0.1 3,876,180 + -1.1 3,876,180 + +1.7 3,876,180 + +1.2 3,876,180 + +0.7 3,876,180 + -1.7 3,876,181 - +1.1 3,876,181 - -0.2 3,876,181 - +0.1 3,876,181 - +1.4 3,876,181 - -0.5 3,876,181 - +0.5 3,876,186 + +0.6 3,876,186 + -1.3 3,876,186 + +1.0 3,876,186 + -0.2 3,876,187 - -0.0 3,876,187 - -0.8 3,876,187 - +0.1 3,876,187 - +0.3 3,876,209 - +1.0 3,876,417 + Pf6N2E2_3348 0.22 -0.5 3,876,417 + Pf6N2E2_3348 0.22 -0.1 3,876,429 - Pf6N2E2_3348 0.24 +1.0 3,876,445 - Pf6N2E2_3348 0.27 -0.1 3,876,462 + Pf6N2E2_3348 0.30 -1.3 3,876,462 + Pf6N2E2_3348 0.30 -1.9 3,876,462 + Pf6N2E2_3348 0.30 -0.0 3,876,462 + Pf6N2E2_3348 0.30 -2.2 3,876,462 + Pf6N2E2_3348 0.30 +0.0 3,876,462 + Pf6N2E2_3348 0.30 +0.6 3,876,463 - Pf6N2E2_3348 0.30 +1.8 3,876,463 - Pf6N2E2_3348 0.30 -0.4 3,876,463 - Pf6N2E2_3348 0.30 +0.5 3,876,463 - Pf6N2E2_3348 0.30 +1.6 3,876,463 - Pf6N2E2_3348 0.30 +2.7
Or see this region's nucleotide sequence