Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3269 and Pf6N2E2_3270 are separated by 2 nucleotides Pf6N2E2_3270 and Pf6N2E2_3271 are separated by 15 nucleotides Pf6N2E2_3271 and Pf6N2E2_3272 are separated by 268 nucleotides Pf6N2E2_3272 and Pf6N2E2_3273 are separated by 4 nucleotides
Pf6N2E2_3269 - PTS IIA-like nitrogen-regulatory protein PtsN, at 3,775,211 to 3,775,633
_3269
Pf6N2E2_3270 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 3,775,636 to 3,776,493
_3270
Pf6N2E2_3271 - Phosphocarrier protein, nitrogen regulation associated, at 3,776,509 to 3,776,784
_3271
Pf6N2E2_3272 - Metal transporter, ZIP family, at 3,777,053 to 3,777,433
_3272
Pf6N2E2_3273 - Metal transporter, ZIP family, at 3,777,438 to 3,777,908
_3273
Position (kb)
3776
3777 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3775.631 kb on + strand at 3775.714 kb on + strand at 3775.714 kb on + strand at 3775.714 kb on + strand at 3775.714 kb on + strand at 3775.714 kb on + strand at 3775.715 kb on - strand at 3775.715 kb on - strand at 3775.822 kb on - strand, within Pf6N2E2_3270 at 3775.822 kb on - strand, within Pf6N2E2_3270 at 3775.881 kb on + strand, within Pf6N2E2_3270 at 3775.900 kb on + strand, within Pf6N2E2_3270 at 3775.901 kb on - strand, within Pf6N2E2_3270 at 3776.016 kb on - strand, within Pf6N2E2_3270 at 3776.016 kb on - strand, within Pf6N2E2_3270 at 3776.055 kb on + strand, within Pf6N2E2_3270 at 3776.055 kb on + strand, within Pf6N2E2_3270 at 3776.056 kb on - strand, within Pf6N2E2_3270 at 3776.306 kb on - strand, within Pf6N2E2_3270 at 3776.324 kb on - strand, within Pf6N2E2_3270 at 3776.369 kb on - strand, within Pf6N2E2_3270 at 3776.369 kb on - strand, within Pf6N2E2_3270 at 3776.392 kb on - strand, within Pf6N2E2_3270 at 3776.491 kb on + strand at 3776.492 kb on - strand at 3776.647 kb on - strand, within Pf6N2E2_3271 at 3776.647 kb on - strand, within Pf6N2E2_3271 at 3776.764 kb on + strand at 3776.765 kb on - strand at 3776.765 kb on - strand at 3776.783 kb on - strand at 3776.783 kb on - strand at 3776.909 kb on - strand at 3777.516 kb on + strand, within Pf6N2E2_3273 at 3777.559 kb on + strand, within Pf6N2E2_3273 at 3777.559 kb on + strand, within Pf6N2E2_3273 at 3777.559 kb on + strand, within Pf6N2E2_3273 at 3777.559 kb on + strand, within Pf6N2E2_3273 at 3777.560 kb on - strand, within Pf6N2E2_3273 at 3777.560 kb on - strand, within Pf6N2E2_3273 at 3777.708 kb on + strand, within Pf6N2E2_3273 at 3777.709 kb on - strand, within Pf6N2E2_3273
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,775,631 + -1.5 3,775,714 + -1.1 3,775,714 + -1.4 3,775,714 + -0.2 3,775,714 + -0.1 3,775,714 + -2.4 3,775,715 - +1.8 3,775,715 - +1.2 3,775,822 - Pf6N2E2_3270 0.22 -2.3 3,775,822 - Pf6N2E2_3270 0.22 -1.1 3,775,881 + Pf6N2E2_3270 0.29 -1.5 3,775,900 + Pf6N2E2_3270 0.31 -0.9 3,775,901 - Pf6N2E2_3270 0.31 -0.1 3,776,016 - Pf6N2E2_3270 0.44 +1.3 3,776,016 - Pf6N2E2_3270 0.44 -0.8 3,776,055 + Pf6N2E2_3270 0.49 +0.6 3,776,055 + Pf6N2E2_3270 0.49 -0.1 3,776,056 - Pf6N2E2_3270 0.49 +1.2 3,776,306 - Pf6N2E2_3270 0.78 +0.0 3,776,324 - Pf6N2E2_3270 0.80 -1.6 3,776,369 - Pf6N2E2_3270 0.85 +0.2 3,776,369 - Pf6N2E2_3270 0.85 +0.4 3,776,392 - Pf6N2E2_3270 0.88 -1.0 3,776,491 + +0.8 3,776,492 - +2.4 3,776,647 - Pf6N2E2_3271 0.50 -0.8 3,776,647 - Pf6N2E2_3271 0.50 -1.9 3,776,764 + +0.6 3,776,765 - -2.6 3,776,765 - +0.6 3,776,783 - +0.2 3,776,783 - +0.2 3,776,909 - +1.2 3,777,516 + Pf6N2E2_3273 0.17 +0.6 3,777,559 + Pf6N2E2_3273 0.26 +0.8 3,777,559 + Pf6N2E2_3273 0.26 +1.0 3,777,559 + Pf6N2E2_3273 0.26 +0.1 3,777,559 + Pf6N2E2_3273 0.26 +0.1 3,777,560 - Pf6N2E2_3273 0.26 +0.2 3,777,560 - Pf6N2E2_3273 0.26 -1.8 3,777,708 + Pf6N2E2_3273 0.57 -0.8 3,777,709 - Pf6N2E2_3273 0.58 -3.0
Or see this region's nucleotide sequence