Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3252 and Pf6N2E2_3253 are separated by 123 nucleotides Pf6N2E2_3253 and Pf6N2E2_3254 are separated by 127 nucleotides Pf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotides Pf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotides Pf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides
Pf6N2E2_3252 - Histidinol dehydrogenase (EC 1.1.1.23), at 3,762,472 to 3,763,809
_3252
Pf6N2E2_3253 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 3,763,933 to 3,764,568
_3253
Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961
_3254
Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223
_3255
Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764
_3256
Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414
_3257
Position (kb)
3764
3765
3766 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3763.852 kb on + strand at 3763.853 kb on - strand at 3763.853 kb on - strand at 3763.889 kb on + strand at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.199 kb on - strand, within Pf6N2E2_3253 at 3764.201 kb on + strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.585 kb on + strand at 3764.593 kb on + strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.697 kb on + strand at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.213 kb on - strand at 3766.317 kb on + strand at 3766.489 kb on + strand, within Pf6N2E2_3256
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,763,852 + -3.1 3,763,853 - +0.0 3,763,853 - -1.0 3,763,889 + -0.8 3,764,159 + Pf6N2E2_3253 0.36 -1.2 3,764,159 + Pf6N2E2_3253 0.36 -0.9 3,764,159 + Pf6N2E2_3253 0.36 -1.1 3,764,160 - Pf6N2E2_3253 0.36 -0.2 3,764,160 - Pf6N2E2_3253 0.36 -0.5 3,764,160 - Pf6N2E2_3253 0.36 +0.6 3,764,199 - Pf6N2E2_3253 0.42 +0.3 3,764,201 + Pf6N2E2_3253 0.42 -1.4 3,764,202 - Pf6N2E2_3253 0.42 +1.1 3,764,202 - Pf6N2E2_3253 0.42 -0.4 3,764,396 + Pf6N2E2_3253 0.73 -1.0 3,764,396 + Pf6N2E2_3253 0.73 -3.1 3,764,396 + Pf6N2E2_3253 0.73 -0.6 3,764,396 + Pf6N2E2_3253 0.73 -0.1 3,764,585 + -0.0 3,764,593 + -2.8 3,764,594 - -0.6 3,764,594 - +0.3 3,764,594 - +0.5 3,764,594 - -2.0 3,764,697 + -1.6 3,766,076 - Pf6N2E2_3255 0.38 -2.1 3,766,076 - Pf6N2E2_3255 0.38 -0.7 3,766,076 - Pf6N2E2_3255 0.38 -1.8 3,766,076 - Pf6N2E2_3255 0.38 -1.1 3,766,076 - Pf6N2E2_3255 0.38 -1.2 3,766,076 - Pf6N2E2_3255 0.38 +0.8 3,766,195 - Pf6N2E2_3255 0.88 -2.0 3,766,195 - Pf6N2E2_3255 0.88 -2.1 3,766,195 - Pf6N2E2_3255 0.88 -3.1 3,766,213 - -2.9 3,766,317 + -2.7 3,766,489 + Pf6N2E2_3256 0.13 -1.9
Or see this region's nucleotide sequence