Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3245 and Pf6N2E2_3246 are separated by 299 nucleotides Pf6N2E2_3246 and Pf6N2E2_3247 are separated by 45 nucleotides
Pf6N2E2_3245 - L-lysine permease, at 3,754,134 to 3,754,763
_3245
Pf6N2E2_3246 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,755,063 to 3,756,028
_3246
Pf6N2E2_3247 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 3,756,074 to 3,757,072
_3247
Position (kb)
3755
3756
3757 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.519 kb on + strand, within Pf6N2E2_3245 at 3754.520 kb on - strand, within Pf6N2E2_3245 at 3754.759 kb on + strand at 3754.759 kb on + strand at 3754.760 kb on - strand at 3754.760 kb on - strand at 3754.823 kb on - strand at 3754.823 kb on - strand at 3754.869 kb on + strand at 3754.869 kb on + strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.877 kb on + strand at 3754.878 kb on - strand at 3754.942 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.002 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.029 kb on - strand at 3755.055 kb on + strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3756.005 kb on - strand at 3756.029 kb on + strand at 3756.030 kb on - strand at 3756.058 kb on + strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.115 kb on - strand at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.368 kb on + strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.531 kb on - strand, within Pf6N2E2_3247 at 3756.894 kb on - strand, within Pf6N2E2_3247
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,754,297 + Pf6N2E2_3245 0.26 -1.8 3,754,297 + Pf6N2E2_3245 0.26 +0.2 3,754,298 - Pf6N2E2_3245 0.26 -1.1 3,754,298 - Pf6N2E2_3245 0.26 +0.1 3,754,434 - Pf6N2E2_3245 0.48 +0.2 3,754,434 - Pf6N2E2_3245 0.48 +0.2 3,754,510 + Pf6N2E2_3245 0.60 +0.7 3,754,510 + Pf6N2E2_3245 0.60 -1.2 3,754,510 + Pf6N2E2_3245 0.60 +0.1 3,754,510 + Pf6N2E2_3245 0.60 +0.1 3,754,510 + Pf6N2E2_3245 0.60 -0.5 3,754,510 + Pf6N2E2_3245 0.60 -0.5 3,754,511 - Pf6N2E2_3245 0.60 +0.0 3,754,511 - Pf6N2E2_3245 0.60 +0.0 3,754,511 - Pf6N2E2_3245 0.60 +0.6 3,754,511 - Pf6N2E2_3245 0.60 +0.1 3,754,511 - Pf6N2E2_3245 0.60 -1.2 3,754,511 - Pf6N2E2_3245 0.60 -1.0 3,754,519 + Pf6N2E2_3245 0.61 -0.4 3,754,520 - Pf6N2E2_3245 0.61 -0.1 3,754,759 + -1.2 3,754,759 + -1.2 3,754,760 - +0.6 3,754,760 - -0.9 3,754,823 - +0.6 3,754,823 - +2.3 3,754,869 + +1.1 3,754,869 + +0.5 3,754,870 - -0.5 3,754,870 - +0.3 3,754,870 - -0.1 3,754,870 - -0.4 3,754,870 - -0.2 3,754,870 - -1.4 3,754,877 + -2.0 3,754,878 - -0.2 3,754,942 + +0.3 3,755,000 + +0.1 3,755,000 + +1.4 3,755,000 + +0.5 3,755,000 + -2.4 3,755,001 - -0.5 3,755,001 - +0.6 3,755,001 - +1.4 3,755,001 - +0.7 3,755,001 - -0.8 3,755,001 - -0.2 3,755,001 - +0.3 3,755,001 - +0.6 3,755,001 - +1.0 3,755,001 - +0.5 3,755,001 - -1.4 3,755,002 + +0.4 3,755,028 + +1.2 3,755,028 + +0.2 3,755,028 + -0.1 3,755,029 - +1.9 3,755,055 + -0.7 3,755,056 - +0.6 3,755,056 - -1.1 3,755,056 - +1.1 3,755,056 - +0.4 3,756,005 - -1.4 3,756,029 + +0.4 3,756,030 - -0.4 3,756,058 + +0.1 3,756,059 - -0.4 3,756,059 - +0.5 3,756,059 - -0.9 3,756,059 - -1.2 3,756,094 - -0.6 3,756,094 - -2.5 3,756,094 - -0.8 3,756,115 - -0.4 3,756,323 + Pf6N2E2_3247 0.25 +1.1 3,756,323 + Pf6N2E2_3247 0.25 -1.1 3,756,324 - Pf6N2E2_3247 0.25 -0.3 3,756,324 - Pf6N2E2_3247 0.25 -0.7 3,756,324 - Pf6N2E2_3247 0.25 +0.0 3,756,368 + Pf6N2E2_3247 0.29 -0.3 3,756,369 - Pf6N2E2_3247 0.30 -0.1 3,756,369 - Pf6N2E2_3247 0.30 +0.5 3,756,369 - Pf6N2E2_3247 0.30 +0.2 3,756,369 - Pf6N2E2_3247 0.30 -0.7 3,756,369 - Pf6N2E2_3247 0.30 -3.4 3,756,369 - Pf6N2E2_3247 0.30 -3.0 3,756,531 - Pf6N2E2_3247 0.46 +0.4 3,756,894 - Pf6N2E2_3247 0.82 -0.4
Or see this region's nucleotide sequence