Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3234 and Pf6N2E2_3235 are separated by 21 nucleotides Pf6N2E2_3235 and Pf6N2E2_3236 are separated by 327 nucleotides Pf6N2E2_3236 and Pf6N2E2_3237 are separated by 24 nucleotides Pf6N2E2_3237 and Pf6N2E2_3238 are separated by 348 nucleotides
Pf6N2E2_3234 - mandelate racemase/muconate lactonizing enzyme family protein, at 3,739,273 to 3,740,334
_3234
Pf6N2E2_3235 - hypothetical protein, at 3,740,356 to 3,740,493
_3235
Pf6N2E2_3236 - hypothetical protein, at 3,740,821 to 3,740,934
_3236
Pf6N2E2_3237 - hypothetical protein, at 3,740,959 to 3,741,186
_3237
Pf6N2E2_3238 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 3,741,535 to 3,742,530
_3238
Position (kb)
3740
3741
3742 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3740.282 kb on + strand at 3740.283 kb on - strand at 3740.628 kb on + strand at 3740.629 kb on - strand at 3740.659 kb on + strand at 3740.666 kb on + strand at 3740.667 kb on - strand at 3740.718 kb on + strand at 3740.788 kb on - strand at 3740.961 kb on - strand at 3741.055 kb on - strand, within Pf6N2E2_3237 at 3741.080 kb on + strand, within Pf6N2E2_3237 at 3741.080 kb on + strand, within Pf6N2E2_3237 at 3741.096 kb on - strand, within Pf6N2E2_3237 at 3741.186 kb on + strand at 3741.186 kb on + strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.261 kb on + strand at 3741.261 kb on + strand at 3741.261 kb on + strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.271 kb on + strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.400 kb on - strand at 3741.457 kb on + strand at 3741.457 kb on + strand at 3741.458 kb on - strand at 3741.518 kb on - strand at 3741.518 kb on - strand at 3741.518 kb on - strand at 3741.745 kb on + strand, within Pf6N2E2_3238 at 3741.745 kb on + strand, within Pf6N2E2_3238 at 3741.746 kb on - strand, within Pf6N2E2_3238 at 3741.826 kb on + strand, within Pf6N2E2_3238 at 3741.962 kb on - strand, within Pf6N2E2_3238 at 3741.962 kb on - strand, within Pf6N2E2_3238 at 3741.962 kb on - strand, within Pf6N2E2_3238 at 3741.986 kb on + strand, within Pf6N2E2_3238 at 3742.095 kb on + strand, within Pf6N2E2_3238 at 3742.120 kb on + strand, within Pf6N2E2_3238 at 3742.120 kb on + strand, within Pf6N2E2_3238 at 3742.120 kb on + strand, within Pf6N2E2_3238 at 3742.121 kb on - strand, within Pf6N2E2_3238 at 3742.177 kb on + strand, within Pf6N2E2_3238 at 3742.178 kb on - strand, within Pf6N2E2_3238
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,740,282 + -0.7 3,740,283 - -0.6 3,740,628 + +0.4 3,740,629 - -0.2 3,740,659 + -0.7 3,740,666 + -1.6 3,740,667 - +0.5 3,740,718 + -0.8 3,740,788 - -0.2 3,740,961 - -2.0 3,741,055 - Pf6N2E2_3237 0.42 +1.8 3,741,080 + Pf6N2E2_3237 0.53 +0.9 3,741,080 + Pf6N2E2_3237 0.53 +1.2 3,741,096 - Pf6N2E2_3237 0.60 -0.2 3,741,186 + +0.7 3,741,186 + -1.0 3,741,187 - -0.1 3,741,187 - -0.0 3,741,187 - -0.3 3,741,187 - -0.5 3,741,187 - +1.0 3,741,187 - -0.1 3,741,204 + -0.0 3,741,204 + -0.7 3,741,204 + +0.8 3,741,204 + -0.5 3,741,205 - +0.0 3,741,205 - +0.8 3,741,205 - -0.5 3,741,205 - +2.5 3,741,205 - -0.6 3,741,205 - -0.4 3,741,205 - -0.2 3,741,205 - -0.7 3,741,261 + -2.5 3,741,261 + +0.0 3,741,261 + +1.7 3,741,262 - +0.6 3,741,262 - +0.7 3,741,262 - +1.0 3,741,262 - -0.7 3,741,262 - +1.0 3,741,262 - -0.1 3,741,262 - +0.6 3,741,271 + +0.0 3,741,272 - +0.1 3,741,272 - +1.2 3,741,272 - +0.3 3,741,272 - +0.2 3,741,272 - +1.3 3,741,272 - -0.6 3,741,272 - +1.0 3,741,272 - +0.8 3,741,400 - -1.0 3,741,457 + +1.2 3,741,457 + -0.5 3,741,458 - +0.3 3,741,518 - +2.2 3,741,518 - +1.0 3,741,518 - +1.1 3,741,745 + Pf6N2E2_3238 0.21 -0.4 3,741,745 + Pf6N2E2_3238 0.21 -0.7 3,741,746 - Pf6N2E2_3238 0.21 -0.2 3,741,826 + Pf6N2E2_3238 0.29 +0.8 3,741,962 - Pf6N2E2_3238 0.43 +0.2 3,741,962 - Pf6N2E2_3238 0.43 +0.1 3,741,962 - Pf6N2E2_3238 0.43 +0.7 3,741,986 + Pf6N2E2_3238 0.45 -2.3 3,742,095 + Pf6N2E2_3238 0.56 +0.0 3,742,120 + Pf6N2E2_3238 0.59 +0.2 3,742,120 + Pf6N2E2_3238 0.59 +0.8 3,742,120 + Pf6N2E2_3238 0.59 +0.7 3,742,121 - Pf6N2E2_3238 0.59 -0.0 3,742,177 + Pf6N2E2_3238 0.64 +0.1 3,742,178 - Pf6N2E2_3238 0.65 +1.2
Or see this region's nucleotide sequence