Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3154 and Pf6N2E2_3155 are separated by 212 nucleotides Pf6N2E2_3155 and Pf6N2E2_3156 are separated by 2 nucleotides Pf6N2E2_3156 and Pf6N2E2_3157 are separated by 143 nucleotides
Pf6N2E2_3154 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 3,647,414 to 3,649,828
_3154
Pf6N2E2_3155 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,650,041 to 3,650,433
_3155
Pf6N2E2_3156 - POSSIBLE MEMBRANE PROTEIN, at 3,650,436 to 3,651,080
_3156
Pf6N2E2_3157 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 3,651,224 to 3,651,967
_3157
Position (kb)
3650
3651 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3649.085 kb on - strand, within Pf6N2E2_3154 at 3649.122 kb on - strand, within Pf6N2E2_3154 at 3649.122 kb on - strand, within Pf6N2E2_3154 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.761 kb on - strand at 3649.761 kb on - strand at 3649.838 kb on - strand at 3649.987 kb on + strand at 3649.988 kb on - strand at 3649.988 kb on - strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.075 kb on + strand at 3650.240 kb on + strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.365 kb on + strand, within Pf6N2E2_3155 at 3650.365 kb on + strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.476 kb on - strand at 3650.476 kb on - strand at 3650.629 kb on + strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.697 kb on + strand, within Pf6N2E2_3156 at 3650.697 kb on + strand, within Pf6N2E2_3156 at 3650.710 kb on - strand, within Pf6N2E2_3156 at 3650.804 kb on - strand, within Pf6N2E2_3156 at 3650.833 kb on - strand, within Pf6N2E2_3156 at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.039 kb on - strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.112 kb on + strand at 3651.113 kb on - strand at 3651.113 kb on - strand at 3651.407 kb on + strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,649,085 - Pf6N2E2_3154 0.69 +0.9 3,649,122 - Pf6N2E2_3154 0.71 -2.8 3,649,122 - Pf6N2E2_3154 0.71 +0.2 3,649,442 + Pf6N2E2_3154 0.84 +0.5 3,649,442 + Pf6N2E2_3154 0.84 -0.1 3,649,442 + Pf6N2E2_3154 0.84 +1.3 3,649,443 - Pf6N2E2_3154 0.84 +1.1 3,649,443 - Pf6N2E2_3154 0.84 +0.0 3,649,443 - Pf6N2E2_3154 0.84 +0.8 3,649,443 - Pf6N2E2_3154 0.84 -0.5 3,649,443 - Pf6N2E2_3154 0.84 +0.4 3,649,443 - Pf6N2E2_3154 0.84 +0.5 3,649,761 - +1.4 3,649,761 - -1.2 3,649,838 - +0.5 3,649,987 + +0.1 3,649,988 - +0.9 3,649,988 - +0.6 3,650,040 + -1.2 3,650,040 + +0.5 3,650,040 + +0.3 3,650,040 + -0.0 3,650,040 + -0.6 3,650,040 + -0.9 3,650,040 + +0.8 3,650,041 - -0.1 3,650,041 - +0.6 3,650,041 - -1.4 3,650,041 - +1.2 3,650,041 - -2.1 3,650,041 - +1.3 3,650,075 + +0.4 3,650,240 + Pf6N2E2_3155 0.51 -1.4 3,650,241 - Pf6N2E2_3155 0.51 -0.2 3,650,241 - Pf6N2E2_3155 0.51 +0.2 3,650,241 - Pf6N2E2_3155 0.51 +3.3 3,650,241 - Pf6N2E2_3155 0.51 -1.5 3,650,365 + Pf6N2E2_3155 0.82 +1.3 3,650,365 + Pf6N2E2_3155 0.82 +0.1 3,650,366 - Pf6N2E2_3155 0.83 +0.6 3,650,366 - Pf6N2E2_3155 0.83 +0.4 3,650,366 - Pf6N2E2_3155 0.83 -0.6 3,650,475 + +0.3 3,650,475 + +0.2 3,650,475 + +1.3 3,650,475 + +1.0 3,650,475 + +0.5 3,650,476 - -1.2 3,650,476 - -1.2 3,650,629 + Pf6N2E2_3156 0.30 -0.6 3,650,630 - Pf6N2E2_3156 0.30 +1.2 3,650,630 - Pf6N2E2_3156 0.30 -0.4 3,650,630 - Pf6N2E2_3156 0.30 -1.5 3,650,630 - Pf6N2E2_3156 0.30 -0.6 3,650,697 + Pf6N2E2_3156 0.40 -0.3 3,650,697 + Pf6N2E2_3156 0.40 +0.8 3,650,710 - Pf6N2E2_3156 0.42 -0.4 3,650,804 - Pf6N2E2_3156 0.57 +0.3 3,650,833 - Pf6N2E2_3156 0.62 -0.1 3,651,038 + +1.2 3,651,038 + -0.3 3,651,038 + -0.4 3,651,039 - -1.1 3,651,078 + -1.1 3,651,078 + +0.9 3,651,078 + +1.4 3,651,078 + -2.8 3,651,078 + -0.2 3,651,079 - -0.3 3,651,079 - +1.3 3,651,079 - -0.7 3,651,079 - +0.7 3,651,112 + -1.3 3,651,113 - -0.1 3,651,113 - -0.3 3,651,407 + Pf6N2E2_3157 0.25 +0.6 3,651,408 - Pf6N2E2_3157 0.25 -0.2 3,651,408 - Pf6N2E2_3157 0.25 +0.1
Or see this region's nucleotide sequence