Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3081 and Pf6N2E2_3082 are separated by 44 nucleotides Pf6N2E2_3082 and Pf6N2E2_3083 overlap by 12 nucleotides Pf6N2E2_3083 and Pf6N2E2_3084 overlap by 1 nucleotides Pf6N2E2_3084 and Pf6N2E2_3085 are separated by 82 nucleotides
Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966
_3081
Pf6N2E2_3082 - hypothetical protein, at 3,570,011 to 3,570,190
_3082
Pf6N2E2_3083 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,570,179 to 3,571,330
_3083
Pf6N2E2_3084 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 3,571,330 to 3,572,139
_3084
Pf6N2E2_3085 - FIG00954614: hypothetical protein, at 3,572,222 to 3,572,599
_3085
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3569.201 kb on + strand at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.918 kb on - strand at 3569.955 kb on - strand at 3569.989 kb on + strand at 3569.989 kb on + strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.991 kb on + strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3570.100 kb on + strand, within Pf6N2E2_3082 at 3570.101 kb on - strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.103 kb on - strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.141 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082 at 3571.525 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3572.117 kb on - strand at 3572.122 kb on + strand at 3572.122 kb on + strand at 3572.123 kb on - strand at 3572.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,569,201 + -1.6 3,569,380 + Pf6N2E2_3081 0.28 -0.9 3,569,380 + Pf6N2E2_3081 0.28 -0.9 3,569,381 - Pf6N2E2_3081 0.28 -0.7 3,569,381 - Pf6N2E2_3081 0.28 +0.4 3,569,433 + Pf6N2E2_3081 0.35 -0.3 3,569,433 + Pf6N2E2_3081 0.35 -1.3 3,569,433 + Pf6N2E2_3081 0.35 -1.7 3,569,434 - Pf6N2E2_3081 0.35 -0.9 3,569,434 - Pf6N2E2_3081 0.35 -1.1 3,569,626 + Pf6N2E2_3081 0.58 +0.2 3,569,626 + Pf6N2E2_3081 0.58 -0.6 3,569,626 + Pf6N2E2_3081 0.58 +0.3 3,569,627 - Pf6N2E2_3081 0.58 +0.3 3,569,627 - Pf6N2E2_3081 0.58 +0.2 3,569,918 - -1.1 3,569,955 - +0.3 3,569,989 + +0.9 3,569,989 + -1.2 3,569,990 - +0.5 3,569,990 - +0.4 3,569,990 - -0.1 3,569,991 + +0.1 3,569,992 - +0.4 3,569,992 - +0.3 3,569,992 - -1.6 3,569,992 - -1.1 3,570,100 + Pf6N2E2_3082 0.49 +0.3 3,570,101 - Pf6N2E2_3082 0.50 -0.3 3,570,102 + Pf6N2E2_3082 0.51 +0.1 3,570,102 + Pf6N2E2_3082 0.51 +0.4 3,570,103 - Pf6N2E2_3082 0.51 -2.4 3,570,107 + Pf6N2E2_3082 0.53 -1.1 3,570,107 + Pf6N2E2_3082 0.53 +1.0 3,570,107 + Pf6N2E2_3082 0.53 +1.3 3,570,107 + Pf6N2E2_3082 0.53 -0.6 3,570,141 + Pf6N2E2_3082 0.72 +0.1 3,570,161 + Pf6N2E2_3082 0.83 -0.1 3,570,161 + Pf6N2E2_3082 0.83 -1.4 3,571,525 + Pf6N2E2_3084 0.24 +0.2 3,571,609 + Pf6N2E2_3084 0.34 +1.0 3,571,609 + Pf6N2E2_3084 0.34 +2.3 3,571,609 + Pf6N2E2_3084 0.34 +0.2 3,571,610 - Pf6N2E2_3084 0.35 +1.0 3,571,610 - Pf6N2E2_3084 0.35 -0.1 3,571,612 + Pf6N2E2_3084 0.35 -0.9 3,571,612 + Pf6N2E2_3084 0.35 -0.3 3,571,613 - Pf6N2E2_3084 0.35 -0.2 3,571,613 - Pf6N2E2_3084 0.35 -0.2 3,571,613 - Pf6N2E2_3084 0.35 +0.1 3,571,909 + Pf6N2E2_3084 0.71 +1.6 3,571,909 + Pf6N2E2_3084 0.71 -0.4 3,571,909 + Pf6N2E2_3084 0.71 +1.3 3,571,910 - Pf6N2E2_3084 0.72 +1.1 3,571,910 - Pf6N2E2_3084 0.72 +0.7 3,572,117 - +1.5 3,572,122 + -0.3 3,572,122 + +0.2 3,572,123 - -1.1 3,572,247 - +0.1
Or see this region's nucleotide sequence