Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2870 and Pf6N2E2_2871 are separated by 246 nucleotides Pf6N2E2_2871 and Pf6N2E2_2872 are separated by 206 nucleotides Pf6N2E2_2872 and Pf6N2E2_2873 are separated by 309 nucleotides
Pf6N2E2_2870 - Ribonuclease BN (EC 3.1.-.-), at 3,357,922 to 3,358,872
_2870
Pf6N2E2_2871 - FIG00954271: hypothetical protein, at 3,359,119 to 3,359,496
_2871
Pf6N2E2_2872 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,359,703 to 3,361,394
_2872
Pf6N2E2_2873 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,361,704 to 3,363,392
_2873
Position (kb)
3359
3360
3361
3362 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3358.784 kb on + strand at 3358.784 kb on + strand at 3358.784 kb on + strand at 3358.940 kb on + strand at 3358.940 kb on + strand at 3358.940 kb on + strand at 3358.941 kb on - strand at 3358.941 kb on - strand at 3359.028 kb on + strand at 3359.042 kb on + strand at 3359.042 kb on + strand at 3359.042 kb on + strand at 3359.043 kb on - strand at 3359.043 kb on - strand at 3359.043 kb on - strand at 3359.043 kb on - strand at 3359.043 kb on - strand at 3359.077 kb on + strand at 3359.078 kb on - strand at 3359.078 kb on - strand at 3359.078 kb on - strand at 3359.078 kb on - strand at 3359.130 kb on + strand at 3359.272 kb on + strand, within Pf6N2E2_2871 at 3359.438 kb on - strand, within Pf6N2E2_2871 at 3359.438 kb on - strand, within Pf6N2E2_2871 at 3359.438 kb on - strand, within Pf6N2E2_2871 at 3359.463 kb on + strand at 3359.463 kb on + strand at 3359.464 kb on - strand at 3359.466 kb on + strand at 3359.466 kb on + strand at 3359.467 kb on - strand at 3359.467 kb on - strand at 3359.995 kb on + strand, within Pf6N2E2_2872 at 3360.044 kb on + strand, within Pf6N2E2_2872 at 3360.052 kb on + strand, within Pf6N2E2_2872 at 3360.052 kb on + strand, within Pf6N2E2_2872 at 3360.052 kb on + strand, within Pf6N2E2_2872 at 3360.470 kb on + strand, within Pf6N2E2_2872 at 3360.471 kb on - strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.604 kb on + strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.605 kb on - strand, within Pf6N2E2_2872 at 3360.614 kb on - strand, within Pf6N2E2_2872 at 3360.754 kb on + strand, within Pf6N2E2_2872 at 3360.755 kb on - strand, within Pf6N2E2_2872 at 3360.755 kb on - strand, within Pf6N2E2_2872 at 3360.860 kb on - strand, within Pf6N2E2_2872 at 3360.883 kb on + strand, within Pf6N2E2_2872 at 3360.883 kb on + strand, within Pf6N2E2_2872 at 3360.883 kb on + strand, within Pf6N2E2_2872 at 3360.883 kb on + strand, within Pf6N2E2_2872 at 3360.883 kb on + strand, within Pf6N2E2_2872 at 3360.884 kb on - strand, within Pf6N2E2_2872 at 3360.884 kb on - strand, within Pf6N2E2_2872 at 3360.884 kb on - strand, within Pf6N2E2_2872 at 3361.179 kb on + strand, within Pf6N2E2_2872 at 3361.228 kb on + strand at 3361.228 kb on + strand at 3361.229 kb on - strand at 3361.240 kb on + strand at 3361.240 kb on + strand at 3361.240 kb on + strand at 3361.241 kb on - strand at 3361.241 kb on - strand at 3361.366 kb on + strand at 3361.366 kb on + strand at 3361.366 kb on + strand at 3361.366 kb on + strand at 3361.366 kb on + strand at 3361.366 kb on + strand at 3361.367 kb on - strand at 3361.370 kb on + strand at 3361.370 kb on + strand at 3361.370 kb on + strand at 3361.370 kb on + strand at 3361.370 kb on + strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.371 kb on - strand at 3361.443 kb on + strand at 3361.444 kb on - strand at 3361.446 kb on + strand at 3361.515 kb on + strand at 3361.516 kb on - strand at 3361.586 kb on + strand at 3361.587 kb on - strand at 3361.788 kb on + strand at 3361.788 kb on + strand at 3361.820 kb on - strand at 3361.970 kb on - strand, within Pf6N2E2_2873 at 3362.117 kb on - strand, within Pf6N2E2_2873
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,358,784 + +0.2 3,358,784 + +1.8 3,358,784 + +1.7 3,358,940 + +1.0 3,358,940 + -0.6 3,358,940 + +0.8 3,358,941 - +0.2 3,358,941 - -0.5 3,359,028 + +1.1 3,359,042 + -0.4 3,359,042 + +0.9 3,359,042 + +1.3 3,359,043 - +0.6 3,359,043 - -1.6 3,359,043 - -0.3 3,359,043 - -1.2 3,359,043 - -0.3 3,359,077 + +0.5 3,359,078 - +0.8 3,359,078 - +1.0 3,359,078 - -0.4 3,359,078 - -0.8 3,359,130 + -0.3 3,359,272 + Pf6N2E2_2871 0.40 +0.1 3,359,438 - Pf6N2E2_2871 0.84 -0.3 3,359,438 - Pf6N2E2_2871 0.84 -2.1 3,359,438 - Pf6N2E2_2871 0.84 -0.2 3,359,463 + -0.7 3,359,463 + +0.6 3,359,464 - +0.5 3,359,466 + +0.9 3,359,466 + +0.6 3,359,467 - +1.1 3,359,467 - -1.2 3,359,995 + Pf6N2E2_2872 0.17 +3.2 3,360,044 + Pf6N2E2_2872 0.20 +3.7 3,360,052 + Pf6N2E2_2872 0.21 +4.2 3,360,052 + Pf6N2E2_2872 0.21 +3.1 3,360,052 + Pf6N2E2_2872 0.21 +2.4 3,360,470 + Pf6N2E2_2872 0.45 +3.8 3,360,471 - Pf6N2E2_2872 0.45 +2.8 3,360,604 + Pf6N2E2_2872 0.53 +4.0 3,360,604 + Pf6N2E2_2872 0.53 +3.6 3,360,604 + Pf6N2E2_2872 0.53 +3.4 3,360,604 + Pf6N2E2_2872 0.53 +2.9 3,360,604 + Pf6N2E2_2872 0.53 +3.7 3,360,604 + Pf6N2E2_2872 0.53 +3.8 3,360,604 + Pf6N2E2_2872 0.53 +3.0 3,360,604 + Pf6N2E2_2872 0.53 +3.1 3,360,605 - Pf6N2E2_2872 0.53 +2.0 3,360,605 - Pf6N2E2_2872 0.53 +3.3 3,360,605 - +3.5 3,360,605 - Pf6N2E2_2872 0.53 +2.3 3,360,605 - Pf6N2E2_2872 0.53 +4.1 3,360,605 - Pf6N2E2_2872 0.53 +2.8 3,360,605 - Pf6N2E2_2872 0.53 +1.9 3,360,605 - Pf6N2E2_2872 0.53 +3.5 3,360,605 - Pf6N2E2_2872 0.53 +3.1 3,360,605 - Pf6N2E2_2872 0.53 +3.7 3,360,605 - Pf6N2E2_2872 0.53 +2.5 3,360,614 - Pf6N2E2_2872 0.54 +3.4 3,360,754 + Pf6N2E2_2872 0.62 +3.4 3,360,755 - Pf6N2E2_2872 0.62 +3.4 3,360,755 - Pf6N2E2_2872 0.62 +4.1 3,360,860 - Pf6N2E2_2872 0.68 +2.9 3,360,883 + Pf6N2E2_2872 0.70 +4.5 3,360,883 + Pf6N2E2_2872 0.70 +2.5 3,360,883 + Pf6N2E2_2872 0.70 +3.8 3,360,883 + Pf6N2E2_2872 0.70 +2.5 3,360,883 + Pf6N2E2_2872 0.70 +3.6 3,360,884 - Pf6N2E2_2872 0.70 +0.2 3,360,884 - Pf6N2E2_2872 0.70 +1.7 3,360,884 - Pf6N2E2_2872 0.70 +2.4 3,361,179 + Pf6N2E2_2872 0.87 +2.2 3,361,228 + +4.3 3,361,228 + +3.9 3,361,229 - +3.4 3,361,240 + +4.0 3,361,240 + +2.8 3,361,240 + +3.2 3,361,241 - +2.0 3,361,241 - +3.1 3,361,366 + +2.3 3,361,366 + +2.5 3,361,366 + +2.5 3,361,366 + +1.8 3,361,366 + +3.4 3,361,366 + +3.1 3,361,367 - +3.5 3,361,370 + +3.2 3,361,370 + +3.3 3,361,370 + +3.5 3,361,370 + +3.6 3,361,370 + +3.5 3,361,371 - +3.4 3,361,371 - +4.0 3,361,371 - +2.3 3,361,371 - +2.6 3,361,371 - +4.0 3,361,371 - +1.8 3,361,371 - +2.7 3,361,371 - +2.8 3,361,371 - +3.8 3,361,443 + +3.5 3,361,444 - +2.9 3,361,446 + +3.0 3,361,515 + +3.2 3,361,516 - -1.8 3,361,586 + -0.9 3,361,587 - +1.0 3,361,788 + +0.1 3,361,788 + -0.2 3,361,820 - +0.0 3,361,970 - Pf6N2E2_2873 0.16 +0.2 3,362,117 - Pf6N2E2_2873 0.24 -1.1
Or see this region's nucleotide sequence