Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2707 and Pf6N2E2_2708 are separated by 8 nucleotides Pf6N2E2_2708 and Pf6N2E2_2709 are separated by 64 nucleotides Pf6N2E2_2709 and Pf6N2E2_2710 are separated by 546 nucleotides
Pf6N2E2_2707 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,200,157 to 3,200,849
_2707
Pf6N2E2_2708 - Lactam utilization protein LamB, at 3,200,858 to 3,201,610
_2708
Pf6N2E2_2709 - Sialic acid transporter (permease) NanT, at 3,201,675 to 3,202,961
_2709
Pf6N2E2_2710 - FIG00953701: hypothetical protein, at 3,203,508 to 3,204,050
_2710
Position (kb)
3201
3202
3203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.709 kb on - strand, within Pf6N2E2_2707 at 3200.710 kb on + strand, within Pf6N2E2_2707 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3201.090 kb on + strand, within Pf6N2E2_2708 at 3201.091 kb on - strand, within Pf6N2E2_2708 at 3201.204 kb on + strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.288 kb on + strand, within Pf6N2E2_2708 at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.643 kb on - strand at 3201.643 kb on - strand at 3201.676 kb on + strand at 3201.676 kb on + strand at 3201.743 kb on - strand at 3201.743 kb on - strand at 3201.847 kb on + strand, within Pf6N2E2_2709 at 3201.882 kb on - strand, within Pf6N2E2_2709 at 3201.971 kb on + strand, within Pf6N2E2_2709 at 3201.993 kb on - strand, within Pf6N2E2_2709 at 3201.994 kb on + strand, within Pf6N2E2_2709 at 3201.994 kb on + strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.070 kb on - strand, within Pf6N2E2_2709 at 3202.070 kb on - strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.121 kb on - strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.192 kb on + strand, within Pf6N2E2_2709 at 3202.192 kb on + strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.270 kb on + strand, within Pf6N2E2_2709 at 3202.270 kb on + strand, within Pf6N2E2_2709 at 3202.317 kb on - strand, within Pf6N2E2_2709 at 3202.318 kb on + strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.607 kb on - strand, within Pf6N2E2_2709 at 3202.607 kb on - strand, within Pf6N2E2_2709 at 3202.739 kb on + strand, within Pf6N2E2_2709 at 3202.739 kb on + strand, within Pf6N2E2_2709 at 3202.740 kb on - strand, within Pf6N2E2_2709 at 3202.740 kb on - strand, within Pf6N2E2_2709 at 3202.772 kb on + strand, within Pf6N2E2_2709 at 3202.772 kb on + strand, within Pf6N2E2_2709 at 3202.773 kb on - strand, within Pf6N2E2_2709 at 3202.980 kb on + strand at 3202.980 kb on + strand at 3202.981 kb on - strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3203.060 kb on + strand at 3203.079 kb on + strand at 3203.080 kb on - strand at 3203.133 kb on + strand at 3203.200 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.328 kb on + strand at 3203.329 kb on - strand at 3203.406 kb on - strand at 3203.674 kb on + strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.681 kb on - strand, within Pf6N2E2_2710 at 3203.747 kb on - strand, within Pf6N2E2_2710
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,200,690 - Pf6N2E2_2707 0.77 +0.9 3,200,690 - Pf6N2E2_2707 0.77 -0.2 3,200,709 - Pf6N2E2_2707 0.80 -0.5 3,200,710 + Pf6N2E2_2707 0.80 +1.1 3,200,954 - Pf6N2E2_2708 0.13 +0.2 3,200,954 - Pf6N2E2_2708 0.13 -1.5 3,200,954 - Pf6N2E2_2708 0.13 -0.5 3,201,090 + Pf6N2E2_2708 0.31 +0.6 3,201,091 - Pf6N2E2_2708 0.31 -0.3 3,201,204 + Pf6N2E2_2708 0.46 -1.5 3,201,265 - Pf6N2E2_2708 0.54 +1.0 3,201,265 - Pf6N2E2_2708 0.54 -0.5 3,201,265 - Pf6N2E2_2708 0.54 -2.7 3,201,265 - Pf6N2E2_2708 0.54 +0.0 3,201,265 - Pf6N2E2_2708 0.54 -0.1 3,201,288 + Pf6N2E2_2708 0.57 +0.6 3,201,626 - +1.9 3,201,626 - -0.4 3,201,626 - +1.0 3,201,626 - +0.2 3,201,643 - +1.1 3,201,643 - +0.8 3,201,676 + +0.2 3,201,676 + -0.8 3,201,743 - -0.7 3,201,743 - +0.1 3,201,847 + Pf6N2E2_2709 0.13 +0.6 3,201,882 - Pf6N2E2_2709 0.16 -1.1 3,201,971 + Pf6N2E2_2709 0.23 +1.0 3,201,993 - Pf6N2E2_2709 0.25 +0.9 3,201,994 + Pf6N2E2_2709 0.25 +0.3 3,201,994 + Pf6N2E2_2709 0.25 +0.3 3,201,995 - Pf6N2E2_2709 0.25 -1.4 3,201,995 - Pf6N2E2_2709 0.25 +2.3 3,201,995 - Pf6N2E2_2709 0.25 +0.5 3,201,995 - Pf6N2E2_2709 0.25 +0.5 3,201,995 - Pf6N2E2_2709 0.25 +1.6 3,201,995 - Pf6N2E2_2709 0.25 -1.3 3,201,995 - Pf6N2E2_2709 0.25 +1.7 3,201,995 - Pf6N2E2_2709 0.25 +0.1 3,202,069 + Pf6N2E2_2709 0.31 +0.5 3,202,069 + Pf6N2E2_2709 0.31 -1.5 3,202,069 + Pf6N2E2_2709 0.31 +0.5 3,202,070 - Pf6N2E2_2709 0.31 +0.9 3,202,070 - Pf6N2E2_2709 0.31 +0.9 3,202,084 + Pf6N2E2_2709 0.32 +1.0 3,202,084 + Pf6N2E2_2709 0.32 -0.1 3,202,084 + Pf6N2E2_2709 0.32 +1.0 3,202,084 + Pf6N2E2_2709 0.32 +0.7 3,202,085 - Pf6N2E2_2709 0.32 +0.9 3,202,085 - Pf6N2E2_2709 0.32 +1.0 3,202,085 - Pf6N2E2_2709 0.32 +1.7 3,202,085 - Pf6N2E2_2709 0.32 +0.0 3,202,093 + Pf6N2E2_2709 0.32 +1.1 3,202,093 + Pf6N2E2_2709 0.32 -0.4 3,202,093 + Pf6N2E2_2709 0.32 -0.2 3,202,094 - Pf6N2E2_2709 0.33 +0.9 3,202,094 - Pf6N2E2_2709 0.33 +2.0 3,202,094 - Pf6N2E2_2709 0.33 +0.2 3,202,121 - Pf6N2E2_2709 0.35 +1.8 3,202,137 + Pf6N2E2_2709 0.36 +0.8 3,202,137 + Pf6N2E2_2709 0.36 +0.1 3,202,137 + Pf6N2E2_2709 0.36 +0.6 3,202,137 + Pf6N2E2_2709 0.36 -0.7 3,202,137 + Pf6N2E2_2709 0.36 +0.4 3,202,137 + Pf6N2E2_2709 0.36 +1.0 3,202,137 + Pf6N2E2_2709 0.36 +1.0 3,202,137 + Pf6N2E2_2709 0.36 +1.0 3,202,137 + Pf6N2E2_2709 0.36 -0.5 3,202,137 + Pf6N2E2_2709 0.36 +0.4 3,202,138 - Pf6N2E2_2709 0.36 +1.4 3,202,138 - Pf6N2E2_2709 0.36 +1.1 3,202,138 - Pf6N2E2_2709 0.36 +1.0 3,202,138 - Pf6N2E2_2709 0.36 -2.0 3,202,138 - Pf6N2E2_2709 0.36 +0.3 3,202,138 - Pf6N2E2_2709 0.36 +0.3 3,202,138 - Pf6N2E2_2709 0.36 -0.0 3,202,138 - Pf6N2E2_2709 0.36 +0.4 3,202,138 - Pf6N2E2_2709 0.36 +1.3 3,202,138 - Pf6N2E2_2709 0.36 +0.8 3,202,192 + Pf6N2E2_2709 0.40 +0.0 3,202,192 + Pf6N2E2_2709 0.40 +0.7 3,202,193 - Pf6N2E2_2709 0.40 +0.0 3,202,193 - Pf6N2E2_2709 0.40 +1.1 3,202,193 - Pf6N2E2_2709 0.40 +1.5 3,202,193 - Pf6N2E2_2709 0.40 +0.5 3,202,270 + Pf6N2E2_2709 0.46 +0.4 3,202,270 + Pf6N2E2_2709 0.46 +0.8 3,202,317 - Pf6N2E2_2709 0.50 +1.1 3,202,318 + Pf6N2E2_2709 0.50 -2.4 3,202,319 - Pf6N2E2_2709 0.50 +1.3 3,202,319 - Pf6N2E2_2709 0.50 +0.2 3,202,319 - Pf6N2E2_2709 0.50 -0.0 3,202,319 - Pf6N2E2_2709 0.50 -0.5 3,202,439 - Pf6N2E2_2709 0.59 -0.7 3,202,439 - Pf6N2E2_2709 0.59 -0.4 3,202,439 - Pf6N2E2_2709 0.59 +0.5 3,202,606 + Pf6N2E2_2709 0.72 -1.7 3,202,606 + Pf6N2E2_2709 0.72 -0.9 3,202,606 + Pf6N2E2_2709 0.72 +0.7 3,202,606 + Pf6N2E2_2709 0.72 +1.8 3,202,606 + Pf6N2E2_2709 0.72 -0.5 3,202,607 - Pf6N2E2_2709 0.72 +1.3 3,202,607 - Pf6N2E2_2709 0.72 -0.0 3,202,739 + Pf6N2E2_2709 0.83 -1.3 3,202,739 + Pf6N2E2_2709 0.83 -0.6 3,202,740 - Pf6N2E2_2709 0.83 +0.7 3,202,740 - Pf6N2E2_2709 0.83 -0.8 3,202,772 + Pf6N2E2_2709 0.85 +0.3 3,202,772 + Pf6N2E2_2709 0.85 +0.4 3,202,773 - Pf6N2E2_2709 0.85 -0.7 3,202,980 + +0.6 3,202,980 + -0.4 3,202,981 - +1.5 3,202,988 + -1.7 3,202,988 + -0.1 3,202,988 + +1.0 3,202,988 + -0.4 3,202,989 - +1.4 3,202,989 - +0.8 3,202,989 - +1.0 3,202,989 - +2.0 3,202,989 - +0.8 3,202,989 - +1.2 3,202,989 - +0.6 3,202,989 - +1.5 3,202,989 - +0.1 3,203,060 + +0.8 3,203,079 + +1.5 3,203,080 - -0.8 3,203,133 + -0.5 3,203,200 + -0.1 3,203,207 + +1.0 3,203,207 + +1.1 3,203,207 + +0.7 3,203,207 + +0.2 3,203,207 + -0.0 3,203,207 + -0.6 3,203,208 - -1.1 3,203,208 - -0.5 3,203,208 - +0.8 3,203,208 - -3.3 3,203,328 + +0.3 3,203,329 - +0.7 3,203,406 - -1.0 3,203,674 + Pf6N2E2_2710 0.31 -0.1 3,203,675 - Pf6N2E2_2710 0.31 +0.9 3,203,675 - Pf6N2E2_2710 0.31 -0.2 3,203,675 - Pf6N2E2_2710 0.31 +0.3 3,203,681 - Pf6N2E2_2710 0.32 +1.5 3,203,747 - Pf6N2E2_2710 0.44 +0.3
Or see this region's nucleotide sequence