Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotides Pf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides Pf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotides Pf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides
Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917
_2579
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127
_2581
Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586
_2582
Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627
_2583
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.709 kb on - strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.862 kb on - strand, within Pf6N2E2_2579 at 3038.903 kb on + strand at 3038.903 kb on + strand at 3038.904 kb on - strand at 3038.917 kb on + strand at 3038.942 kb on + strand at 3038.943 kb on - strand at 3039.207 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.692 kb on - strand at 3039.692 kb on - strand at 3039.855 kb on + strand, within Pf6N2E2_2581 at 3040.242 kb on + strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.617 kb on - strand at 3040.617 kb on - strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.972 kb on + strand, within Pf6N2E2_2583 at 3040.973 kb on - strand, within Pf6N2E2_2583 at 3041.095 kb on + strand, within Pf6N2E2_2583 at 3041.096 kb on - strand, within Pf6N2E2_2583
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 3,038,691 + Pf6N2E2_2579 0.16 -1.4 3,038,691 + Pf6N2E2_2579 0.16 +0.5 3,038,691 + Pf6N2E2_2579 0.16 +1.7 3,038,691 + Pf6N2E2_2579 0.16 -0.1 3,038,692 - Pf6N2E2_2579 0.16 -1.1 3,038,692 - Pf6N2E2_2579 0.16 -0.1 3,038,692 - Pf6N2E2_2579 0.16 +0.4 3,038,692 - Pf6N2E2_2579 0.16 +0.3 3,038,709 - Pf6N2E2_2579 0.23 +1.7 3,038,861 + Pf6N2E2_2579 0.79 -0.9 3,038,861 + Pf6N2E2_2579 0.79 +0.9 3,038,862 - Pf6N2E2_2579 0.79 +0.7 3,038,903 + +0.4 3,038,903 + -2.5 3,038,904 - -2.6 3,038,917 + +0.5 3,038,942 + -0.3 3,038,943 - +0.6 3,039,207 + Pf6N2E2_2580 0.35 +0.8 3,039,335 + Pf6N2E2_2580 0.53 -3.4 3,039,335 + Pf6N2E2_2580 0.53 +0.4 3,039,336 - Pf6N2E2_2580 0.53 -2.2 3,039,336 - Pf6N2E2_2580 0.53 -0.1 3,039,569 + Pf6N2E2_2580 0.84 +0.3 3,039,569 + Pf6N2E2_2580 0.84 +0.5 3,039,692 - -0.6 3,039,692 - -0.6 3,039,855 + Pf6N2E2_2581 0.38 +2.2 3,040,242 + Pf6N2E2_2582 0.25 +1.0 3,040,243 - Pf6N2E2_2582 0.25 +0.9 3,040,243 - Pf6N2E2_2582 0.25 -0.8 3,040,243 - Pf6N2E2_2582 0.25 -0.3 3,040,617 - -0.2 3,040,617 - -1.4 3,040,641 + -0.9 3,040,641 + +1.0 3,040,641 + +0.9 3,040,972 + Pf6N2E2_2583 0.32 -0.6 3,040,973 - Pf6N2E2_2583 0.32 +0.3 3,041,095 + Pf6N2E2_2583 0.45 +0.1 3,041,096 - Pf6N2E2_2583 0.45 -0.4
Or see this region's nucleotide sequence