Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2530 and Pf6N2E2_2531 are separated by 190 nucleotides Pf6N2E2_2531 and Pf6N2E2_2532 overlap by 8 nucleotides Pf6N2E2_2532 and Pf6N2E2_2533 are separated by 3 nucleotides
Pf6N2E2_2530 - Sensory box/GGDEF family protein, at 2,986,968 to 2,989,427
_2530
Pf6N2E2_2531 - Lipid A export ATP-binding/permease protein MsbA, at 2,989,618 to 2,991,444
_2531
Pf6N2E2_2532 - Thioredoxin, at 2,991,437 to 2,992,072
_2532
Pf6N2E2_2533 - Rhodanese domain protein UPF0176, at 2,992,076 to 2,993,017
_2533
Position (kb)
2989
2990
2991
2992 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2988.622 kb on + strand, within Pf6N2E2_2530 at 2988.623 kb on - strand, within Pf6N2E2_2530 at 2988.746 kb on + strand, within Pf6N2E2_2530 at 2988.746 kb on + strand, within Pf6N2E2_2530 at 2989.201 kb on + strand at 2989.201 kb on + strand at 2989.202 kb on - strand at 2989.202 kb on - strand at 2989.238 kb on - strand at 2989.238 kb on - strand at 2989.249 kb on + strand at 2989.250 kb on - strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.252 kb on + strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.253 kb on - strand at 2989.325 kb on - strand at 2989.325 kb on - strand at 2989.357 kb on - strand at 2989.389 kb on + strand at 2989.446 kb on - strand at 2989.493 kb on + strand at 2989.493 kb on + strand at 2989.494 kb on - strand at 2989.498 kb on + strand at 2989.499 kb on - strand at 2989.595 kb on - strand at 2989.595 kb on - strand at 2989.595 kb on - strand at 2989.649 kb on + strand at 2989.649 kb on + strand at 2989.649 kb on + strand at 2989.649 kb on + strand at 2989.650 kb on - strand at 2989.650 kb on - strand at 2989.650 kb on - strand at 2989.662 kb on - strand at 2989.827 kb on - strand, within Pf6N2E2_2531 at 2989.891 kb on + strand, within Pf6N2E2_2531 at 2989.891 kb on + strand, within Pf6N2E2_2531 at 2989.892 kb on - strand, within Pf6N2E2_2531 at 2990.077 kb on - strand, within Pf6N2E2_2531 at 2990.192 kb on + strand, within Pf6N2E2_2531 at 2990.192 kb on + strand, within Pf6N2E2_2531 at 2990.193 kb on - strand, within Pf6N2E2_2531 at 2990.385 kb on - strand, within Pf6N2E2_2531 at 2990.448 kb on - strand, within Pf6N2E2_2531 at 2990.456 kb on + strand, within Pf6N2E2_2531 at 2990.456 kb on + strand, within Pf6N2E2_2531 at 2990.457 kb on - strand, within Pf6N2E2_2531 at 2990.504 kb on + strand, within Pf6N2E2_2531 at 2990.505 kb on - strand, within Pf6N2E2_2531 at 2990.581 kb on - strand, within Pf6N2E2_2531 at 2990.654 kb on + strand, within Pf6N2E2_2531 at 2990.834 kb on + strand, within Pf6N2E2_2531 at 2990.835 kb on - strand, within Pf6N2E2_2531 at 2990.835 kb on - strand, within Pf6N2E2_2531 at 2991.081 kb on - strand, within Pf6N2E2_2531 at 2991.186 kb on - strand, within Pf6N2E2_2531 at 2991.286 kb on + strand at 2991.287 kb on - strand at 2991.357 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.423 kb on + strand at 2991.424 kb on - strand at 2991.424 kb on - strand at 2991.424 kb on - strand at 2991.424 kb on - strand at 2991.717 kb on + strand, within Pf6N2E2_2532 at 2991.717 kb on + strand, within Pf6N2E2_2532 at 2991.717 kb on + strand, within Pf6N2E2_2532 at 2991.718 kb on - strand, within Pf6N2E2_2532 at 2991.718 kb on - strand, within Pf6N2E2_2532 at 2991.814 kb on - strand, within Pf6N2E2_2532 at 2991.897 kb on + strand, within Pf6N2E2_2532 at 2991.897 kb on + strand, within Pf6N2E2_2532 at 2991.898 kb on - strand, within Pf6N2E2_2532 at 2991.992 kb on + strand, within Pf6N2E2_2532 at 2991.993 kb on - strand, within Pf6N2E2_2532 at 2992.053 kb on + strand at 2992.053 kb on + strand at 2992.053 kb on + strand at 2992.053 kb on + strand at 2992.053 kb on + strand at 2992.054 kb on - strand at 2992.054 kb on - strand at 2992.101 kb on + strand at 2992.101 kb on + strand at 2992.101 kb on + strand at 2992.102 kb on - strand at 2992.102 kb on - strand at 2992.102 kb on - strand at 2992.102 kb on - strand at 2992.233 kb on + strand, within Pf6N2E2_2533 at 2992.233 kb on + strand, within Pf6N2E2_2533 at 2992.233 kb on + strand, within Pf6N2E2_2533 at 2992.233 kb on + strand, within Pf6N2E2_2533 at 2992.233 kb on + strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.234 kb on - strand, within Pf6N2E2_2533 at 2992.256 kb on + strand, within Pf6N2E2_2533 at 2992.256 kb on + strand, within Pf6N2E2_2533 at 2992.257 kb on - strand, within Pf6N2E2_2533 at 2992.257 kb on - strand, within Pf6N2E2_2533 at 2992.257 kb on - strand, within Pf6N2E2_2533 at 2992.257 kb on - strand, within Pf6N2E2_2533 at 2992.257 kb on - strand, within Pf6N2E2_2533 at 2992.270 kb on + strand, within Pf6N2E2_2533 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.419 kb on + strand, within Pf6N2E2_2533 at 2992.419 kb on + strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,988,622 + Pf6N2E2_2530 0.67 -0.1 2,988,623 - Pf6N2E2_2530 0.67 -0.6 2,988,746 + Pf6N2E2_2530 0.72 -0.4 2,988,746 + Pf6N2E2_2530 0.72 +0.4 2,989,201 + -1.9 2,989,201 + -0.5 2,989,202 - +0.8 2,989,202 - -0.8 2,989,238 - -0.7 2,989,238 - -0.1 2,989,249 + -1.0 2,989,250 - -1.8 2,989,252 + +0.3 2,989,252 + -0.5 2,989,252 + -3.6 2,989,252 + -0.1 2,989,252 + +0.0 2,989,252 + -1.1 2,989,252 + +0.1 2,989,253 - -0.7 2,989,253 - -1.9 2,989,253 - +0.2 2,989,253 - +0.1 2,989,253 - -0.0 2,989,253 - -0.8 2,989,253 - -0.1 2,989,253 - +0.2 2,989,253 - +0.4 2,989,253 - +0.5 2,989,253 - +0.7 2,989,253 - -1.9 2,989,253 - -0.9 2,989,253 - -0.8 2,989,253 - +0.2 2,989,253 - -0.9 2,989,325 - +0.2 2,989,325 - -0.3 2,989,357 - +0.7 2,989,389 + -3.1 2,989,446 - -0.3 2,989,493 + +1.2 2,989,493 + -1.5 2,989,494 - -0.4 2,989,498 + -1.5 2,989,499 - +1.6 2,989,595 - -1.5 2,989,595 - -0.1 2,989,595 - +0.3 2,989,649 + -0.1 2,989,649 + -0.4 2,989,649 + +0.0 2,989,649 + -0.5 2,989,650 - -0.0 2,989,650 - -0.3 2,989,650 - -0.5 2,989,662 - +1.1 2,989,827 - Pf6N2E2_2531 0.11 -0.1 2,989,891 + Pf6N2E2_2531 0.15 -2.8 2,989,891 + Pf6N2E2_2531 0.15 -1.4 2,989,892 - Pf6N2E2_2531 0.15 +0.3 2,990,077 - Pf6N2E2_2531 0.25 +0.6 2,990,192 + Pf6N2E2_2531 0.31 +0.1 2,990,192 + Pf6N2E2_2531 0.31 -1.2 2,990,193 - Pf6N2E2_2531 0.31 +0.6 2,990,385 - Pf6N2E2_2531 0.42 -0.3 2,990,448 - Pf6N2E2_2531 0.45 +0.1 2,990,456 + Pf6N2E2_2531 0.46 -1.4 2,990,456 + Pf6N2E2_2531 0.46 -1.1 2,990,457 - Pf6N2E2_2531 0.46 +0.3 2,990,504 + Pf6N2E2_2531 0.48 +1.0 2,990,505 - Pf6N2E2_2531 0.49 +1.1 2,990,581 - Pf6N2E2_2531 0.53 +1.4 2,990,654 + Pf6N2E2_2531 0.57 -0.3 2,990,834 + Pf6N2E2_2531 0.67 -0.0 2,990,835 - Pf6N2E2_2531 0.67 -0.1 2,990,835 - Pf6N2E2_2531 0.67 +0.0 2,991,081 - Pf6N2E2_2531 0.80 +2.3 2,991,186 - Pf6N2E2_2531 0.86 -0.1 2,991,286 + -0.5 2,991,287 - -0.4 2,991,357 + -0.6 2,991,423 + -0.7 2,991,423 + -0.1 2,991,423 + -0.7 2,991,423 + -0.7 2,991,423 + +0.7 2,991,423 + -0.5 2,991,423 + -0.5 2,991,424 - +0.1 2,991,424 - -1.7 2,991,424 - +0.5 2,991,424 - -1.4 2,991,717 + Pf6N2E2_2532 0.44 +0.3 2,991,717 + Pf6N2E2_2532 0.44 +0.2 2,991,717 + Pf6N2E2_2532 0.44 -0.4 2,991,718 - Pf6N2E2_2532 0.44 +0.3 2,991,718 - Pf6N2E2_2532 0.44 +0.3 2,991,814 - Pf6N2E2_2532 0.59 -1.8 2,991,897 + Pf6N2E2_2532 0.72 +0.8 2,991,897 + Pf6N2E2_2532 0.72 -0.5 2,991,898 - Pf6N2E2_2532 0.72 +0.1 2,991,992 + Pf6N2E2_2532 0.87 -0.2 2,991,993 - Pf6N2E2_2532 0.87 +0.2 2,992,053 + -0.7 2,992,053 + +0.5 2,992,053 + -2.4 2,992,053 + -1.5 2,992,053 + -0.7 2,992,054 - +0.2 2,992,054 - +0.3 2,992,101 + +1.3 2,992,101 + +0.6 2,992,101 + +0.1 2,992,102 - -0.9 2,992,102 - +0.9 2,992,102 - +0.2 2,992,102 - -0.8 2,992,233 + Pf6N2E2_2533 0.17 -0.2 2,992,233 + Pf6N2E2_2533 0.17 +0.5 2,992,233 + Pf6N2E2_2533 0.17 -0.1 2,992,233 + Pf6N2E2_2533 0.17 +0.0 2,992,233 + Pf6N2E2_2533 0.17 +1.5 2,992,234 - Pf6N2E2_2533 0.17 +0.0 2,992,234 - Pf6N2E2_2533 0.17 +1.1 2,992,234 - Pf6N2E2_2533 0.17 -0.5 2,992,234 - Pf6N2E2_2533 0.17 +0.7 2,992,234 - Pf6N2E2_2533 0.17 -0.5 2,992,234 - Pf6N2E2_2533 0.17 -2.4 2,992,234 - Pf6N2E2_2533 0.17 -1.7 2,992,234 - Pf6N2E2_2533 0.17 +0.1 2,992,256 + Pf6N2E2_2533 0.19 +0.6 2,992,256 + Pf6N2E2_2533 0.19 +1.0 2,992,257 - Pf6N2E2_2533 0.19 +0.2 2,992,257 - Pf6N2E2_2533 0.19 +0.9 2,992,257 - Pf6N2E2_2533 0.19 -0.0 2,992,257 - Pf6N2E2_2533 0.19 -0.2 2,992,257 - Pf6N2E2_2533 0.19 +0.5 2,992,270 + Pf6N2E2_2533 0.21 +0.9 2,992,294 - Pf6N2E2_2533 0.23 -0.4 2,992,294 - Pf6N2E2_2533 0.23 +0.6 2,992,294 - Pf6N2E2_2533 0.23 +0.6 2,992,419 + Pf6N2E2_2533 0.36 +1.5 2,992,419 + Pf6N2E2_2533 0.36 +1.5 2,992,420 - Pf6N2E2_2533 0.37 -1.7 2,992,420 - Pf6N2E2_2533 0.37 -2.3 2,992,420 - Pf6N2E2_2533 0.37 +0.3
Or see this region's nucleotide sequence