Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2524 and Pf6N2E2_2525 are separated by 61 nucleotides Pf6N2E2_2525 and Pf6N2E2_2526 are separated by 4 nucleotides Pf6N2E2_2526 and Pf6N2E2_2527 are separated by 167 nucleotides
Pf6N2E2_2524 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 2,981,025 to 2,982,356
_2524
Pf6N2E2_2525 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 2,982,418 to 2,983,116
_2525
Pf6N2E2_2526 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 2,983,121 to 2,983,792
_2526
Pf6N2E2_2527 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,983,960 to 2,984,700
_2527
Position (kb)
2983
2984 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2982.186 kb on + strand, within Pf6N2E2_2524 at 2982.186 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.210 kb on + strand, within Pf6N2E2_2524 at 2982.232 kb on + strand at 2982.354 kb on + strand at 2983.114 kb on + strand at 2983.114 kb on + strand at 2983.115 kb on - strand at 2983.115 kb on - strand at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.688 kb on + strand, within Pf6N2E2_2526 at 2983.689 kb on - strand, within Pf6N2E2_2526 at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.959 kb on + strand at 2983.959 kb on + strand at 2983.960 kb on - strand at 2984.057 kb on - strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.280 kb on + strand, within Pf6N2E2_2527 at 2984.355 kb on + strand, within Pf6N2E2_2527 at 2984.444 kb on - strand, within Pf6N2E2_2527 at 2984.455 kb on + strand, within Pf6N2E2_2527 at 2984.644 kb on + strand at 2984.727 kb on + strand at 2984.727 kb on + strand at 2984.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,982,186 + Pf6N2E2_2524 0.87 +0.4 2,982,186 + Pf6N2E2_2524 0.87 +0.4 2,982,210 + Pf6N2E2_2524 0.89 +1.5 2,982,210 + Pf6N2E2_2524 0.89 +1.4 2,982,210 + Pf6N2E2_2524 0.89 +1.6 2,982,210 + Pf6N2E2_2524 0.89 +2.7 2,982,210 + Pf6N2E2_2524 0.89 +1.2 2,982,210 + Pf6N2E2_2524 0.89 +1.2 2,982,210 + Pf6N2E2_2524 0.89 +1.3 2,982,210 + Pf6N2E2_2524 0.89 +2.0 2,982,210 + Pf6N2E2_2524 0.89 +2.4 2,982,210 + Pf6N2E2_2524 0.89 +1.5 2,982,232 + +2.0 2,982,354 + +1.3 2,983,114 + +1.4 2,983,114 + -0.3 2,983,115 - +0.9 2,983,115 - -0.8 2,983,682 + Pf6N2E2_2526 0.83 -1.5 2,983,682 + Pf6N2E2_2526 0.83 -2.6 2,983,682 + Pf6N2E2_2526 0.83 -2.3 2,983,682 + Pf6N2E2_2526 0.83 -2.5 2,983,682 + Pf6N2E2_2526 0.83 -1.7 2,983,682 + Pf6N2E2_2526 0.83 -3.8 2,983,683 - Pf6N2E2_2526 0.84 -1.1 2,983,683 - Pf6N2E2_2526 0.84 -3.7 2,983,688 + Pf6N2E2_2526 0.84 -2.4 2,983,689 - Pf6N2E2_2526 0.85 -2.7 2,983,942 + -0.3 2,983,942 + -1.1 2,983,942 + +0.5 2,983,959 + +2.4 2,983,959 + -0.0 2,983,960 - -2.1 2,984,057 - Pf6N2E2_2527 0.13 -2.7 2,984,119 + Pf6N2E2_2527 0.21 +0.8 2,984,119 + Pf6N2E2_2527 0.21 +1.3 2,984,197 + Pf6N2E2_2527 0.32 -0.7 2,984,197 + Pf6N2E2_2527 0.32 -0.1 2,984,198 - Pf6N2E2_2527 0.32 +0.1 2,984,198 - Pf6N2E2_2527 0.32 +0.1 2,984,280 + Pf6N2E2_2527 0.43 -0.4 2,984,355 + Pf6N2E2_2527 0.53 -2.3 2,984,444 - Pf6N2E2_2527 0.65 -1.4 2,984,455 + Pf6N2E2_2527 0.67 -1.3 2,984,644 + -0.3 2,984,727 + +1.4 2,984,727 + +0.4 2,984,728 - +1.0
Or see this region's nucleotide sequence