Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2513 and Pf6N2E2_2514 are separated by 168 nucleotides Pf6N2E2_2514 and Pf6N2E2_2515 are separated by 430 nucleotides
Pf6N2E2_2513 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 2,964,520 to 2,965,803
_2513
Pf6N2E2_2514 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,965,972 to 2,967,021
_2514
Pf6N2E2_2515 - Integration host factor beta subunit, at 2,967,452 to 2,967,748
_2515
Position (kb)
2965
2966
2967
2968 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2964.996 kb on - strand, within Pf6N2E2_2513 at 2965.015 kb on - strand, within Pf6N2E2_2513 at 2965.196 kb on - strand, within Pf6N2E2_2513 at 2965.713 kb on - strand at 2965.862 kb on - strand at 2965.865 kb on - strand at 2965.865 kb on - strand at 2965.885 kb on - strand at 2965.885 kb on - strand at 2965.939 kb on - strand at 2965.944 kb on - strand at 2966.008 kb on - strand at 2966.057 kb on - strand at 2966.108 kb on - strand, within Pf6N2E2_2514 at 2966.305 kb on - strand, within Pf6N2E2_2514 at 2966.344 kb on - strand, within Pf6N2E2_2514 at 2966.381 kb on - strand, within Pf6N2E2_2514 at 2966.479 kb on + strand, within Pf6N2E2_2514 at 2966.480 kb on - strand, within Pf6N2E2_2514 at 2966.491 kb on - strand, within Pf6N2E2_2514 at 2966.528 kb on + strand, within Pf6N2E2_2514 at 2966.585 kb on + strand, within Pf6N2E2_2514 at 2966.586 kb on - strand, within Pf6N2E2_2514 at 2966.693 kb on - strand, within Pf6N2E2_2514 at 2967.125 kb on + strand at 2967.163 kb on + strand at 2967.163 kb on + strand at 2967.164 kb on - strand at 2967.203 kb on - strand at 2967.252 kb on + strand at 2967.252 kb on + strand at 2967.257 kb on + strand at 2967.327 kb on + strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.491 kb on - strand, within Pf6N2E2_2515 at 2967.799 kb on - strand at 2967.830 kb on + strand at 2967.947 kb on - strand at 2967.992 kb on - strand at 2967.992 kb on - strand at 2968.004 kb on + strand at 2968.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,964,996 - Pf6N2E2_2513 0.37 -1.7 2,965,015 - Pf6N2E2_2513 0.39 -3.1 2,965,196 - Pf6N2E2_2513 0.53 -3.6 2,965,713 - -0.2 2,965,862 - +0.3 2,965,865 - +0.0 2,965,865 - +0.9 2,965,885 - -0.3 2,965,885 - +0.8 2,965,939 - -2.5 2,965,944 - -0.3 2,966,008 - +1.5 2,966,057 - -0.9 2,966,108 - Pf6N2E2_2514 0.13 -2.6 2,966,305 - Pf6N2E2_2514 0.32 -3.1 2,966,344 - Pf6N2E2_2514 0.35 -1.8 2,966,381 - Pf6N2E2_2514 0.39 -1.4 2,966,479 + Pf6N2E2_2514 0.48 -0.1 2,966,480 - Pf6N2E2_2514 0.48 +0.4 2,966,491 - Pf6N2E2_2514 0.49 -0.9 2,966,528 + Pf6N2E2_2514 0.53 +0.5 2,966,585 + Pf6N2E2_2514 0.58 -1.7 2,966,586 - Pf6N2E2_2514 0.58 -2.7 2,966,693 - Pf6N2E2_2514 0.69 -3.0 2,967,125 + -0.8 2,967,163 + -1.2 2,967,163 + -2.1 2,967,164 - -1.7 2,967,203 - -0.0 2,967,252 + -1.1 2,967,252 + -1.6 2,967,257 + -1.1 2,967,327 + +0.1 2,967,328 - +0.3 2,967,328 - -0.4 2,967,328 - +0.1 2,967,328 - +0.5 2,967,491 - Pf6N2E2_2515 0.13 +1.0 2,967,799 - -0.4 2,967,830 + -0.2 2,967,947 - +1.3 2,967,992 - -0.6 2,967,992 - -0.2 2,968,004 + -0.7 2,968,005 - +0.3
Or see this region's nucleotide sequence