Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2509 and Pf6N2E2_2510 are separated by 485 nucleotides Pf6N2E2_2510 and Pf6N2E2_2511 are separated by 57 nucleotides Pf6N2E2_2511 and Pf6N2E2_2512 are separated by 92 nucleotides
Pf6N2E2_2509 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,961,069 to 2,961,572
_2509
Pf6N2E2_2510 - Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase, at 2,962,058 to 2,963,188
_2510
Pf6N2E2_2511 - hypothetical protein, at 2,963,246 to 2,963,362
_2511
Pf6N2E2_2512 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,963,455 to 2,964,477
_2512
Position (kb)
2962
2963
2964 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2961.113 kb on + strand at 2961.114 kb on - strand at 2961.217 kb on - strand, within Pf6N2E2_2509 at 2961.342 kb on - strand, within Pf6N2E2_2509 at 2961.632 kb on - strand at 2961.873 kb on - strand at 2961.972 kb on - strand at 2962.002 kb on + strand at 2962.042 kb on - strand at 2962.256 kb on + strand, within Pf6N2E2_2510 at 2962.258 kb on + strand, within Pf6N2E2_2510 at 2962.262 kb on - strand, within Pf6N2E2_2510 at 2962.364 kb on + strand, within Pf6N2E2_2510 at 2962.365 kb on - strand, within Pf6N2E2_2510 at 2962.365 kb on - strand, within Pf6N2E2_2510 at 2962.370 kb on - strand, within Pf6N2E2_2510 at 2962.370 kb on - strand, within Pf6N2E2_2510 at 2962.386 kb on - strand, within Pf6N2E2_2510 at 2962.449 kb on - strand, within Pf6N2E2_2510 at 2962.449 kb on - strand, within Pf6N2E2_2510 at 2962.678 kb on + strand, within Pf6N2E2_2510 at 2962.678 kb on + strand, within Pf6N2E2_2510 at 2962.679 kb on - strand, within Pf6N2E2_2510 at 2962.778 kb on - strand, within Pf6N2E2_2510 at 2963.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,961,113 + -2.1 2,961,114 - +0.6 2,961,217 - Pf6N2E2_2509 0.29 -2.8 2,961,342 - Pf6N2E2_2509 0.54 -4.9 2,961,632 - -3.9 2,961,873 - -2.5 2,961,972 - -1.4 2,962,002 + -3.1 2,962,042 - +0.5 2,962,256 + Pf6N2E2_2510 0.18 -2.9 2,962,258 + Pf6N2E2_2510 0.18 -3.2 2,962,262 - Pf6N2E2_2510 0.18 +1.6 2,962,364 + Pf6N2E2_2510 0.27 -0.1 2,962,365 - Pf6N2E2_2510 0.27 -1.1 2,962,365 - Pf6N2E2_2510 0.27 -2.9 2,962,370 - Pf6N2E2_2510 0.28 -2.4 2,962,370 - Pf6N2E2_2510 0.28 -1.9 2,962,386 - Pf6N2E2_2510 0.29 -2.1 2,962,449 - Pf6N2E2_2510 0.35 -1.7 2,962,449 - Pf6N2E2_2510 0.35 -1.1 2,962,678 + Pf6N2E2_2510 0.55 -2.7 2,962,678 + Pf6N2E2_2510 0.55 +0.0 2,962,679 - Pf6N2E2_2510 0.55 -2.8 2,962,778 - Pf6N2E2_2510 0.64 -1.1 2,963,202 - -0.1
Or see this region's nucleotide sequence