Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_250 and Pf6N2E2_251 are separated by 184 nucleotides Pf6N2E2_251 and Pf6N2E2_252 overlap by 39 nucleotides
Pf6N2E2_250 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 311,560 to 312,495
_250
Pf6N2E2_251 - transcriptional regulator, at 312,680 to 314,167
_251
Pf6N2E2_252 - Disease resistance protein / Tetratricopeptide repeat-containing protein, at 314,129 to 316,918
_252
Position (kb) 312
313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 311.753 kb on - strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.933 kb on - strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 312.578 kb on + strand at 312.619 kb on - strand at 312.653 kb on + strand at 312.839 kb on + strand, within Pf6N2E2_251 at 312.962 kb on + strand, within Pf6N2E2_251 at 312.962 kb on + strand at 312.962 kb on + strand, within Pf6N2E2_251 at 312.963 kb on - strand, within Pf6N2E2_251 at 312.989 kb on + strand, within Pf6N2E2_251 at 313.734 kb on + strand, within Pf6N2E2_251 at 313.735 kb on - strand, within Pf6N2E2_251 at 313.735 kb on - strand, within Pf6N2E2_251 at 313.929 kb on + strand, within Pf6N2E2_251 at 314.027 kb on + strand at 314.027 kb on + strand at 314.028 kb on - strand at 314.028 kb on - strand at 314.028 kb on - strand at 314.028 kb on - strand at 314.082 kb on - strand at 314.655 kb on + strand, within Pf6N2E2_252 at 314.691 kb on + strand, within Pf6N2E2_252 at 314.827 kb on - strand, within Pf6N2E2_252 at 314.827 kb on - strand, within Pf6N2E2_252 at 315.036 kb on + strand, within Pf6N2E2_252 at 315.056 kb on + strand, within Pf6N2E2_252 at 315.056 kb on + strand, within Pf6N2E2_252
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 311,753 - Pf6N2E2_250 0.21 +0.9 311,788 + Pf6N2E2_250 0.24 +0.3 311,788 + Pf6N2E2_250 0.24 -0.8 311,788 + Pf6N2E2_250 0.24 -1.6 311,788 + Pf6N2E2_250 0.24 -0.2 311,933 - Pf6N2E2_250 0.40 -0.4 311,987 + Pf6N2E2_250 0.46 -0.3 311,987 + Pf6N2E2_250 0.46 +0.1 311,987 + Pf6N2E2_250 0.46 -3.5 311,987 + Pf6N2E2_250 0.46 +1.3 312,578 + +0.3 312,619 - -0.8 312,653 + +0.6 312,839 + Pf6N2E2_251 0.11 -1.1 312,962 + Pf6N2E2_251 0.19 -3.0 312,962 + -0.7 312,962 + Pf6N2E2_251 0.19 -0.1 312,963 - Pf6N2E2_251 0.19 -1.8 312,989 + Pf6N2E2_251 0.21 +0.8 313,734 + Pf6N2E2_251 0.71 -1.0 313,735 - Pf6N2E2_251 0.71 +1.4 313,735 - Pf6N2E2_251 0.71 -1.3 313,929 + Pf6N2E2_251 0.84 -0.7 314,027 + +1.0 314,027 + +0.0 314,028 - +0.6 314,028 - -0.7 314,028 - -0.2 314,028 - +1.5 314,082 - +0.6 314,655 + Pf6N2E2_252 0.19 -0.2 314,691 + Pf6N2E2_252 0.20 +0.9 314,827 - Pf6N2E2_252 0.25 +1.8 314,827 - Pf6N2E2_252 0.25 -0.1 315,036 + Pf6N2E2_252 0.33 +0.1 315,056 + Pf6N2E2_252 0.33 -0.5 315,056 + Pf6N2E2_252 0.33 +0.7
Or see this region's nucleotide sequence