Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2424 and Pf6N2E2_2425 are separated by 75 nucleotides Pf6N2E2_2425 and Pf6N2E2_2426 are separated by 78 nucleotides Pf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotides Pf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotides Pf6N2E2_2428 and Pf6N2E2_2429 are separated by 189 nucleotides
Pf6N2E2_2424 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,874,663 to 2,875,265
_2424
Pf6N2E2_2425 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,875,341 to 2,875,562
_2425
Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531
_2426
Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like) (from data) , at 2,876,604 to 2,876,924
_2427
Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253
_2428
Pf6N2E2_2429 - Glutathione-regulated potassium-efflux system protein KefB, at 2,877,443 to 2,879,155
_2429
Position (kb)
2875
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2874.840 kb on - strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.862 kb on - strand, within Pf6N2E2_2424 at 2874.951 kb on + strand, within Pf6N2E2_2424 at 2874.952 kb on - strand, within Pf6N2E2_2424 at 2875.235 kb on - strand at 2875.247 kb on + strand at 2875.247 kb on + strand at 2875.248 kb on - strand at 2875.269 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.292 kb on + strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.293 kb on - strand at 2875.294 kb on + strand at 2875.294 kb on + strand at 2875.294 kb on + strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.295 kb on - strand at 2875.310 kb on + strand at 2875.467 kb on + strand, within Pf6N2E2_2425 at 2875.467 kb on + strand, within Pf6N2E2_2425 at 2875.488 kb on + strand, within Pf6N2E2_2425 at 2875.488 kb on + strand, within Pf6N2E2_2425 at 2875.489 kb on - strand, within Pf6N2E2_2425 at 2875.544 kb on + strand at 2875.544 kb on + strand at 2875.545 kb on - strand at 2875.560 kb on + strand at 2875.561 kb on - strand at 2875.584 kb on - strand at 2875.616 kb on - strand at 2875.616 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.654 kb on - strand at 2875.671 kb on + strand at 2875.671 kb on + strand at 2875.671 kb on + strand at 2875.672 kb on - strand at 2875.672 kb on - strand at 2875.722 kb on + strand at 2875.774 kb on + strand, within Pf6N2E2_2426 at 2875.775 kb on - strand, within Pf6N2E2_2426 at 2875.775 kb on - strand, within Pf6N2E2_2426 at 2875.852 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.973 kb on + strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2875.974 kb on - strand, within Pf6N2E2_2426 at 2876.062 kb on + strand, within Pf6N2E2_2426 at 2876.063 kb on - strand, within Pf6N2E2_2426 at 2876.261 kb on - strand, within Pf6N2E2_2426 at 2876.315 kb on - strand, within Pf6N2E2_2426 at 2876.315 kb on - strand, within Pf6N2E2_2426 at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.489 kb on + strand at 2876.490 kb on - strand at 2876.490 kb on - strand at 2876.542 kb on + strand at 2876.563 kb on + strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.941 kb on + strand at 2876.993 kb on - strand at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.200 kb on + strand, within Pf6N2E2_2428 at 2877.288 kb on + strand at 2877.288 kb on + strand at 2877.407 kb on + strand at 2877.421 kb on + strand at 2877.421 kb on + strand at 2877.530 kb on + strand at 2877.531 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,874,840 - Pf6N2E2_2424 0.29 -1.0 2,874,861 + Pf6N2E2_2424 0.33 +0.4 2,874,861 + Pf6N2E2_2424 0.33 -0.9 2,874,861 + Pf6N2E2_2424 0.33 -1.7 2,874,862 - Pf6N2E2_2424 0.33 -0.7 2,874,951 + Pf6N2E2_2424 0.48 -0.6 2,874,952 - Pf6N2E2_2424 0.48 +0.4 2,875,235 - -0.1 2,875,247 + +0.3 2,875,247 + -0.2 2,875,248 - +0.2 2,875,269 + +0.4 2,875,292 + -0.3 2,875,292 + -0.5 2,875,292 + -0.2 2,875,292 + -0.1 2,875,292 + -0.4 2,875,292 + +0.8 2,875,292 + -1.4 2,875,292 + +1.9 2,875,292 + -0.5 2,875,292 + -0.1 2,875,293 - +0.4 2,875,293 - +0.1 2,875,293 - +1.2 2,875,293 - +0.6 2,875,293 - -0.8 2,875,293 - -0.4 2,875,294 + -0.2 2,875,294 + +1.4 2,875,294 + +0.2 2,875,295 - -0.2 2,875,295 - +0.3 2,875,295 - +1.5 2,875,295 - +0.8 2,875,310 + -3.6 2,875,467 + Pf6N2E2_2425 0.57 +1.8 2,875,467 + Pf6N2E2_2425 0.57 +1.9 2,875,488 + Pf6N2E2_2425 0.66 +0.8 2,875,488 + Pf6N2E2_2425 0.66 +0.9 2,875,489 - Pf6N2E2_2425 0.67 -0.0 2,875,544 + -1.5 2,875,544 + +0.4 2,875,545 - -1.0 2,875,560 + -0.3 2,875,561 - +0.5 2,875,584 - +1.0 2,875,616 - +0.9 2,875,616 - +1.4 2,875,654 - +0.2 2,875,654 - +0.1 2,875,654 - -0.1 2,875,654 - +0.1 2,875,671 + -0.7 2,875,671 + -0.3 2,875,671 + -0.9 2,875,672 - -0.2 2,875,672 - +0.8 2,875,722 + -0.2 2,875,774 + Pf6N2E2_2426 0.15 -1.0 2,875,775 - Pf6N2E2_2426 0.15 -0.9 2,875,775 - Pf6N2E2_2426 0.15 -0.7 2,875,852 + Pf6N2E2_2426 0.24 -2.3 2,875,973 + Pf6N2E2_2426 0.37 -0.6 2,875,973 + Pf6N2E2_2426 0.37 -1.6 2,875,973 + Pf6N2E2_2426 0.37 -1.0 2,875,973 + Pf6N2E2_2426 0.37 -0.1 2,875,973 + Pf6N2E2_2426 0.37 +1.0 2,875,973 + Pf6N2E2_2426 0.37 -1.7 2,875,973 + Pf6N2E2_2426 0.37 -1.0 2,875,973 + Pf6N2E2_2426 0.37 +1.4 2,875,973 + Pf6N2E2_2426 0.37 -0.2 2,875,973 + Pf6N2E2_2426 0.37 -0.2 2,875,973 + Pf6N2E2_2426 0.37 -1.9 2,875,973 + Pf6N2E2_2426 0.37 -1.0 2,875,973 + Pf6N2E2_2426 0.37 -0.7 2,875,974 - Pf6N2E2_2426 0.37 +0.1 2,875,974 - Pf6N2E2_2426 0.37 -0.0 2,875,974 - Pf6N2E2_2426 0.37 -1.8 2,875,974 - Pf6N2E2_2426 0.37 -0.6 2,875,974 - Pf6N2E2_2426 0.37 -2.0 2,875,974 - Pf6N2E2_2426 0.37 +0.5 2,875,974 - Pf6N2E2_2426 0.37 -0.6 2,875,974 - Pf6N2E2_2426 0.37 -1.0 2,875,974 - Pf6N2E2_2426 0.37 -0.8 2,875,974 - Pf6N2E2_2426 0.37 -0.8 2,875,974 - Pf6N2E2_2426 0.37 +1.9 2,875,974 - Pf6N2E2_2426 0.37 +1.2 2,875,974 - Pf6N2E2_2426 0.37 -2.5 2,876,062 + Pf6N2E2_2426 0.47 -1.7 2,876,063 - Pf6N2E2_2426 0.47 -0.7 2,876,261 - Pf6N2E2_2426 0.70 -1.5 2,876,315 - Pf6N2E2_2426 0.76 +1.2 2,876,315 - Pf6N2E2_2426 0.76 +0.1 2,876,482 + +0.7 2,876,482 + +2.4 2,876,482 + -0.2 2,876,489 + -0.9 2,876,490 - +0.8 2,876,490 - +0.0 2,876,542 + -1.5 2,876,563 + +0.2 2,876,564 - +0.8 2,876,564 - -0.4 2,876,564 - +0.8 2,876,695 + Pf6N2E2_2427 0.28 +1.4 2,876,695 + Pf6N2E2_2427 0.28 -0.8 2,876,696 - Pf6N2E2_2427 0.29 +1.2 2,876,696 - Pf6N2E2_2427 0.29 +0.9 2,876,696 - Pf6N2E2_2427 0.29 -0.2 2,876,696 - Pf6N2E2_2427 0.29 +2.5 2,876,696 - Pf6N2E2_2427 0.29 -0.9 2,876,696 - Pf6N2E2_2427 0.29 +1.2 2,876,696 - Pf6N2E2_2427 0.29 +0.7 2,876,696 - Pf6N2E2_2427 0.29 +0.5 2,876,696 - Pf6N2E2_2427 0.29 +0.3 2,876,696 - Pf6N2E2_2427 0.29 +1.0 2,876,704 + Pf6N2E2_2427 0.31 +2.8 2,876,704 + Pf6N2E2_2427 0.31 +0.6 2,876,705 - Pf6N2E2_2427 0.31 +0.9 2,876,705 - Pf6N2E2_2427 0.31 +0.9 2,876,705 - Pf6N2E2_2427 0.31 -0.6 2,876,941 + +0.1 2,876,993 - +0.9 2,877,111 + Pf6N2E2_2428 0.40 -0.0 2,877,111 + Pf6N2E2_2428 0.40 -0.2 2,877,111 + Pf6N2E2_2428 0.40 -0.1 2,877,200 + Pf6N2E2_2428 0.77 -0.3 2,877,288 + -0.2 2,877,288 + +0.8 2,877,407 + +0.4 2,877,421 + +1.1 2,877,421 + +0.9 2,877,530 + -1.3 2,877,531 - -1.5
Or see this region's nucleotide sequence