Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2367 and Pf6N2E2_2368 overlap by 4 nucleotides Pf6N2E2_2368 and Pf6N2E2_2369 overlap by 17 nucleotides Pf6N2E2_2369 and Pf6N2E2_2370 are separated by 59 nucleotides
Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870
_2367
Pf6N2E2_2368 - Surface presentation of antigens protein SpaM, at 2,801,867 to 2,802,322
_2368
Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556
_2369
Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673
_2370
Position (kb)
2802
2803
2804 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2802.616 kb on + strand, within Pf6N2E2_2369 at 2802.617 kb on - strand, within Pf6N2E2_2369 at 2802.694 kb on + strand, within Pf6N2E2_2369 at 2802.695 kb on - strand, within Pf6N2E2_2369 at 2802.934 kb on - strand, within Pf6N2E2_2369 at 2803.158 kb on + strand, within Pf6N2E2_2369 at 2803.159 kb on - strand, within Pf6N2E2_2369 at 2803.533 kb on + strand at 2803.533 kb on + strand at 2803.534 kb on - strand at 2803.590 kb on - strand at 2803.590 kb on - strand at 2803.944 kb on + strand, within Pf6N2E2_2370 at 2804.112 kb on + strand, within Pf6N2E2_2370 at 2804.260 kb on - strand, within Pf6N2E2_2370 at 2804.505 kb on + strand, within Pf6N2E2_2370
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,801,664 - Pf6N2E2_2367 0.76 -1.1 2,801,664 - Pf6N2E2_2367 0.76 +1.6 2,802,616 + Pf6N2E2_2369 0.25 -0.5 2,802,617 - Pf6N2E2_2369 0.25 +0.8 2,802,694 + Pf6N2E2_2369 0.31 -0.6 2,802,695 - Pf6N2E2_2369 0.31 +1.3 2,802,934 - Pf6N2E2_2369 0.50 -2.2 2,803,158 + Pf6N2E2_2369 0.68 -0.4 2,803,159 - Pf6N2E2_2369 0.68 +0.2 2,803,533 + -1.0 2,803,533 + -2.7 2,803,534 - -0.5 2,803,590 - -1.1 2,803,590 - +0.6 2,803,944 + Pf6N2E2_2370 0.16 -0.6 2,804,112 + Pf6N2E2_2370 0.24 -3.1 2,804,260 - Pf6N2E2_2370 0.31 +1.1 2,804,505 + Pf6N2E2_2370 0.43 +0.7
Or see this region's nucleotide sequence