Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2347 and Pf6N2E2_2348 are separated by 98 nucleotides
Pf6N2E2_2347 - Dihydroorotate dehydrogenase (EC 1.3.3.1), at 2,783,052 to 2,784,071
_2347
Pf6N2E2_2348 - hypothetical protein, at 2,784,170 to 2,785,534
_2348
Position (kb)
2783
2784
2785 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2782.122 kb on - strand at 2782.132 kb on - strand at 2782.195 kb on + strand at 2782.199 kb on - strand at 2782.240 kb on + strand at 2782.240 kb on + strand at 2782.240 kb on + strand at 2782.266 kb on + strand at 2782.266 kb on + strand at 2782.267 kb on - strand at 2782.267 kb on - strand at 2782.306 kb on + strand at 2782.306 kb on + strand at 2782.307 kb on - strand at 2782.307 kb on - strand at 2782.311 kb on - strand at 2782.327 kb on + strand at 2782.327 kb on + strand at 2782.342 kb on - strand at 2782.380 kb on + strand at 2782.497 kb on + strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.525 kb on + strand at 2782.526 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.566 kb on + strand at 2782.566 kb on + strand at 2782.575 kb on - strand at 2782.581 kb on - strand at 2782.594 kb on + strand at 2782.594 kb on + strand at 2782.618 kb on + strand at 2782.619 kb on - strand at 2782.619 kb on - strand at 2782.619 kb on - strand at 2782.623 kb on + strand at 2782.687 kb on + strand at 2782.711 kb on + strand at 2782.711 kb on + strand at 2782.712 kb on - strand at 2782.751 kb on + strand at 2782.751 kb on + strand at 2782.752 kb on - strand at 2782.752 kb on - strand at 2782.752 kb on - strand at 2782.856 kb on + strand at 2782.856 kb on + strand at 2782.857 kb on - strand at 2782.857 kb on - strand at 2782.870 kb on + strand at 2782.871 kb on - strand at 2782.871 kb on - strand at 2782.871 kb on - strand at 2783.048 kb on + strand at 2783.048 kb on + strand at 2783.048 kb on + strand at 2783.049 kb on - strand at 2783.049 kb on - strand at 2783.049 kb on - strand at 2784.068 kb on - strand at 2784.111 kb on - strand at 2784.112 kb on + strand at 2784.112 kb on + strand at 2784.112 kb on + strand at 2784.113 kb on - strand at 2784.113 kb on - strand at 2784.144 kb on + strand at 2784.144 kb on + strand at 2784.144 kb on + strand at 2784.145 kb on - strand at 2784.145 kb on - strand at 2784.181 kb on + strand at 2784.181 kb on + strand at 2784.181 kb on + strand at 2784.181 kb on + strand at 2784.182 kb on - strand at 2784.182 kb on - strand at 2784.182 kb on - strand at 2784.184 kb on + strand at 2784.185 kb on - strand at 2784.185 kb on - strand at 2784.219 kb on - strand at 2784.219 kb on - strand at 2784.341 kb on + strand, within Pf6N2E2_2348 at 2784.341 kb on + strand, within Pf6N2E2_2348 at 2784.342 kb on - strand, within Pf6N2E2_2348 at 2784.342 kb on - strand, within Pf6N2E2_2348 at 2784.342 kb on - strand, within Pf6N2E2_2348 at 2784.342 kb on - strand, within Pf6N2E2_2348 at 2784.347 kb on + strand, within Pf6N2E2_2348 at 2784.348 kb on - strand, within Pf6N2E2_2348 at 2784.392 kb on + strand, within Pf6N2E2_2348 at 2784.392 kb on + strand, within Pf6N2E2_2348 at 2784.392 kb on + strand, within Pf6N2E2_2348 at 2784.392 kb on + strand, within Pf6N2E2_2348 at 2784.393 kb on - strand, within Pf6N2E2_2348 at 2784.393 kb on - strand, within Pf6N2E2_2348 at 2784.393 kb on - strand, within Pf6N2E2_2348 at 2784.464 kb on + strand, within Pf6N2E2_2348 at 2784.465 kb on - strand, within Pf6N2E2_2348 at 2784.500 kb on + strand, within Pf6N2E2_2348 at 2784.500 kb on + strand, within Pf6N2E2_2348 at 2784.500 kb on + strand, within Pf6N2E2_2348 at 2784.512 kb on + strand, within Pf6N2E2_2348 at 2784.513 kb on - strand, within Pf6N2E2_2348 at 2784.513 kb on - strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.544 kb on + strand, within Pf6N2E2_2348 at 2784.545 kb on - strand, within Pf6N2E2_2348 at 2784.545 kb on - strand, within Pf6N2E2_2348 at 2784.545 kb on - strand, within Pf6N2E2_2348 at 2784.545 kb on - strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.548 kb on + strand, within Pf6N2E2_2348 at 2784.549 kb on - strand, within Pf6N2E2_2348 at 2784.549 kb on - strand, within Pf6N2E2_2348 at 2784.609 kb on + strand, within Pf6N2E2_2348 at 2784.609 kb on + strand, within Pf6N2E2_2348 at 2784.609 kb on + strand, within Pf6N2E2_2348 at 2784.610 kb on - strand, within Pf6N2E2_2348 at 2784.693 kb on + strand, within Pf6N2E2_2348 at 2784.693 kb on + strand, within Pf6N2E2_2348 at 2784.693 kb on + strand, within Pf6N2E2_2348 at 2784.789 kb on + strand, within Pf6N2E2_2348 at 2784.790 kb on - strand, within Pf6N2E2_2348 at 2784.790 kb on - strand, within Pf6N2E2_2348 at 2784.790 kb on - strand, within Pf6N2E2_2348 at 2784.811 kb on + strand, within Pf6N2E2_2348 at 2784.811 kb on + strand, within Pf6N2E2_2348 at 2784.812 kb on - strand, within Pf6N2E2_2348 at 2784.812 kb on - strand, within Pf6N2E2_2348 at 2785.013 kb on - strand, within Pf6N2E2_2348 at 2785.013 kb on - strand, within Pf6N2E2_2348 at 2785.028 kb on - strand, within Pf6N2E2_2348 at 2785.053 kb on + strand, within Pf6N2E2_2348 at 2785.053 kb on + strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,782,122 - +0.8 2,782,132 - +0.0 2,782,195 + -0.4 2,782,199 - +0.4 2,782,240 + -0.3 2,782,240 + +1.5 2,782,240 + +0.7 2,782,266 + -0.8 2,782,266 + -0.5 2,782,267 - +0.6 2,782,267 - -0.9 2,782,306 + +0.5 2,782,306 + +0.2 2,782,307 - +1.7 2,782,307 - +0.4 2,782,311 - -0.1 2,782,327 + +0.8 2,782,327 + -0.6 2,782,342 - +0.2 2,782,380 + -0.0 2,782,497 + -0.9 2,782,498 - -0.2 2,782,498 - -2.1 2,782,498 - -0.7 2,782,498 - -0.0 2,782,525 + +0.1 2,782,526 - +1.6 2,782,538 - +1.0 2,782,538 - -1.4 2,782,538 - -0.2 2,782,538 - -0.7 2,782,538 - +0.2 2,782,566 + -2.1 2,782,566 + +1.3 2,782,575 - -1.0 2,782,581 - +1.2 2,782,594 + +0.5 2,782,594 + +3.1 2,782,618 + -0.3 2,782,619 - +0.5 2,782,619 - -0.2 2,782,619 - -0.2 2,782,623 + -2.8 2,782,687 + +0.8 2,782,711 + -1.0 2,782,711 + +0.0 2,782,712 - -2.1 2,782,751 + -0.1 2,782,751 + +1.6 2,782,752 - +0.6 2,782,752 - -1.4 2,782,752 - +0.1 2,782,856 + -0.9 2,782,856 + -0.2 2,782,857 - +0.8 2,782,857 - +1.2 2,782,870 + +0.1 2,782,871 - -0.0 2,782,871 - -1.4 2,782,871 - +1.3 2,783,048 + -0.3 2,783,048 + -0.8 2,783,048 + -0.5 2,783,049 - +0.2 2,783,049 - +0.9 2,783,049 - +1.4 2,784,068 - -2.5 2,784,111 - -2.2 2,784,112 + -2.6 2,784,112 + -0.9 2,784,112 + -1.0 2,784,113 - +0.2 2,784,113 - -0.4 2,784,144 + +0.8 2,784,144 + -0.5 2,784,144 + -0.5 2,784,145 - -1.6 2,784,145 - +0.1 2,784,181 + -0.4 2,784,181 + +0.1 2,784,181 + +0.6 2,784,181 + +1.0 2,784,182 - +0.1 2,784,182 - -0.8 2,784,182 - +0.2 2,784,184 + -0.2 2,784,185 - -0.5 2,784,185 - -1.1 2,784,219 - -0.1 2,784,219 - -1.1 2,784,341 + Pf6N2E2_2348 0.13 +0.1 2,784,341 + Pf6N2E2_2348 0.13 -1.1 2,784,342 - Pf6N2E2_2348 0.13 +1.1 2,784,342 - Pf6N2E2_2348 0.13 -0.1 2,784,342 - Pf6N2E2_2348 0.13 +0.1 2,784,342 - Pf6N2E2_2348 0.13 +0.9 2,784,347 + Pf6N2E2_2348 0.13 -0.9 2,784,348 - Pf6N2E2_2348 0.13 -0.5 2,784,392 + Pf6N2E2_2348 0.16 +0.6 2,784,392 + Pf6N2E2_2348 0.16 +0.6 2,784,392 + Pf6N2E2_2348 0.16 +0.5 2,784,392 + Pf6N2E2_2348 0.16 -0.3 2,784,393 - Pf6N2E2_2348 0.16 +0.2 2,784,393 - Pf6N2E2_2348 0.16 -0.1 2,784,393 - Pf6N2E2_2348 0.16 -1.3 2,784,464 + Pf6N2E2_2348 0.22 -0.8 2,784,465 - Pf6N2E2_2348 0.22 +0.7 2,784,500 + Pf6N2E2_2348 0.24 +1.6 2,784,500 + Pf6N2E2_2348 0.24 -0.7 2,784,500 + Pf6N2E2_2348 0.24 -1.0 2,784,512 + Pf6N2E2_2348 0.25 -0.9 2,784,513 - Pf6N2E2_2348 0.25 +0.5 2,784,513 - Pf6N2E2_2348 0.25 -0.4 2,784,544 + Pf6N2E2_2348 0.27 +1.1 2,784,544 + Pf6N2E2_2348 0.27 +1.1 2,784,544 + Pf6N2E2_2348 0.27 +0.6 2,784,544 + Pf6N2E2_2348 0.27 +0.2 2,784,544 + Pf6N2E2_2348 0.27 +0.0 2,784,544 + Pf6N2E2_2348 0.27 +0.4 2,784,545 - Pf6N2E2_2348 0.27 -0.4 2,784,545 - Pf6N2E2_2348 0.27 -1.2 2,784,545 - Pf6N2E2_2348 0.27 -2.2 2,784,545 - Pf6N2E2_2348 0.27 -2.5 2,784,548 + Pf6N2E2_2348 0.28 +0.2 2,784,548 + Pf6N2E2_2348 0.28 +0.5 2,784,548 + Pf6N2E2_2348 0.28 -1.5 2,784,548 + Pf6N2E2_2348 0.28 +0.7 2,784,548 + Pf6N2E2_2348 0.28 +0.7 2,784,548 + Pf6N2E2_2348 0.28 +1.5 2,784,549 - Pf6N2E2_2348 0.28 +2.1 2,784,549 - Pf6N2E2_2348 0.28 -0.1 2,784,609 + Pf6N2E2_2348 0.32 +0.6 2,784,609 + Pf6N2E2_2348 0.32 -0.9 2,784,609 + Pf6N2E2_2348 0.32 -0.0 2,784,610 - Pf6N2E2_2348 0.32 +0.2 2,784,693 + Pf6N2E2_2348 0.38 +0.5 2,784,693 + Pf6N2E2_2348 0.38 +1.1 2,784,693 + Pf6N2E2_2348 0.38 +0.7 2,784,789 + Pf6N2E2_2348 0.45 -1.5 2,784,790 - Pf6N2E2_2348 0.45 +1.0 2,784,790 - Pf6N2E2_2348 0.45 -0.8 2,784,790 - Pf6N2E2_2348 0.45 -1.5 2,784,811 + Pf6N2E2_2348 0.47 -0.5 2,784,811 + Pf6N2E2_2348 0.47 -1.6 2,784,812 - Pf6N2E2_2348 0.47 +1.5 2,784,812 - Pf6N2E2_2348 0.47 +1.5 2,785,013 - Pf6N2E2_2348 0.62 -2.9 2,785,013 - Pf6N2E2_2348 0.62 -1.8 2,785,028 - Pf6N2E2_2348 0.63 +1.8 2,785,053 + Pf6N2E2_2348 0.65 -0.4 2,785,053 + Pf6N2E2_2348 0.65 -1.4 2,785,054 - Pf6N2E2_2348 0.65 +0.4 2,785,054 - Pf6N2E2_2348 0.65 +0.7 2,785,054 - Pf6N2E2_2348 0.65 -0.7
Or see this region's nucleotide sequence