Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2283 and Pf6N2E2_2284 are separated by 10 nucleotides Pf6N2E2_2284 and Pf6N2E2_2285 are separated by 71 nucleotides Pf6N2E2_2285 and Pf6N2E2_2286 are separated by 106 nucleotides Pf6N2E2_2286 and Pf6N2E2_2287 are separated by 234 nucleotides
Pf6N2E2_2283 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 2,702,706 to 2,703,314
_2283
Pf6N2E2_2284 - Cell division inhibitor-related protein, at 2,703,325 to 2,703,798
_2284
Pf6N2E2_2285 - FIG00954079: hypothetical protein, at 2,703,870 to 2,704,103
_2285
Pf6N2E2_2286 - FIG00953287: hypothetical protein, at 2,704,210 to 2,704,734
_2286
Pf6N2E2_2287 - DNA topoisomerase I (EC 5.99.1.2), at 2,704,969 to 2,707,581
_2287
Position (kb)
2704
2705 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2703.324 kb on + strand at 2703.465 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.547 kb on - strand, within Pf6N2E2_2284 at 2703.784 kb on - strand at 2703.990 kb on + strand, within Pf6N2E2_2285 at 2703.991 kb on - strand, within Pf6N2E2_2285 at 2704.086 kb on + strand at 2704.097 kb on + strand at 2704.097 kb on + strand at 2704.098 kb on - strand at 2704.098 kb on - strand at 2704.103 kb on + strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.139 kb on - strand at 2704.139 kb on - strand at 2704.192 kb on - strand at 2704.545 kb on - strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.587 kb on - strand, within Pf6N2E2_2286 at 2704.694 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.719 kb on - strand at 2704.719 kb on - strand at 2704.719 kb on - strand at 2704.839 kb on - strand at 2705.006 kb on + strand at 2705.007 kb on - strand at 2705.009 kb on + strand at 2705.010 kb on - strand at 2705.054 kb on + strand at 2705.055 kb on - strand at 2705.261 kb on + strand, within Pf6N2E2_2287 at 2705.261 kb on + strand, within Pf6N2E2_2287 at 2705.261 kb on + strand at 2705.262 kb on - strand, within Pf6N2E2_2287 at 2705.262 kb on - strand, within Pf6N2E2_2287
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,703,324 + -0.2 2,703,465 - Pf6N2E2_2284 0.30 +1.0 2,703,547 - Pf6N2E2_2284 0.47 -0.1 2,703,547 - Pf6N2E2_2284 0.47 +0.2 2,703,547 - Pf6N2E2_2284 0.47 +0.7 2,703,784 - +0.0 2,703,990 + Pf6N2E2_2285 0.51 +1.1 2,703,991 - Pf6N2E2_2285 0.52 +1.4 2,704,086 + +1.5 2,704,097 + +1.8 2,704,097 + +4.2 2,704,098 - +1.5 2,704,098 - +1.1 2,704,103 + +1.1 2,704,104 - +2.3 2,704,104 - +1.4 2,704,104 - -0.1 2,704,104 - +1.6 2,704,104 - +0.2 2,704,134 + +2.9 2,704,134 + +2.2 2,704,134 + +1.5 2,704,135 - +0.7 2,704,135 - +2.0 2,704,135 - +1.6 2,704,135 - +1.7 2,704,135 - -0.8 2,704,135 - +1.4 2,704,135 - +2.1 2,704,135 - +0.1 2,704,135 - +3.9 2,704,135 - +1.8 2,704,135 - -0.8 2,704,136 + +2.0 2,704,136 + +1.4 2,704,136 + -0.9 2,704,136 + +1.8 2,704,136 + +0.8 2,704,136 + +2.4 2,704,136 + +1.6 2,704,136 + +0.9 2,704,137 - +0.7 2,704,137 - -1.9 2,704,137 - +0.3 2,704,137 - +1.1 2,704,137 - -2.2 2,704,137 - -0.7 2,704,137 - +0.9 2,704,137 - +0.8 2,704,137 - +0.1 2,704,137 - +1.6 2,704,137 - -1.5 2,704,137 - -2.0 2,704,137 - -0.8 2,704,137 - +0.0 2,704,138 + +0.5 2,704,138 + +2.5 2,704,138 + +1.1 2,704,138 + -1.5 2,704,139 - -0.2 2,704,139 - -0.4 2,704,192 - +0.1 2,704,545 - Pf6N2E2_2286 0.64 -1.2 2,704,586 + Pf6N2E2_2286 0.72 -3.6 2,704,586 + Pf6N2E2_2286 0.72 +0.2 2,704,587 - Pf6N2E2_2286 0.72 -1.7 2,704,694 + +0.6 2,704,718 + +1.6 2,704,718 + +1.2 2,704,718 + -0.1 2,704,719 - +0.6 2,704,719 - +1.1 2,704,719 - +0.3 2,704,839 - -1.1 2,705,006 + -0.7 2,705,007 - +3.0 2,705,009 + +2.2 2,705,010 - +1.6 2,705,054 + +1.6 2,705,055 - +2.1 2,705,261 + Pf6N2E2_2287 0.11 +2.6 2,705,261 + Pf6N2E2_2287 0.11 +3.2 2,705,261 + +4.8 2,705,262 - Pf6N2E2_2287 0.11 +2.6 2,705,262 - Pf6N2E2_2287 0.11 +2.9
Or see this region's nucleotide sequence