Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_224 and Pf6N2E2_225 are separated by 88 nucleotides Pf6N2E2_225 and Pf6N2E2_226 are separated by 74 nucleotides Pf6N2E2_226 and Pf6N2E2_227 are separated by 22 nucleotides
Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216
_224
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Pf6N2E2_226 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 287,858 to 288,706
_226
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Position (kb) 286
287
288 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.697 kb on - strand, within Pf6N2E2_224 at 285.748 kb on + strand, within Pf6N2E2_224 at 285.952 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.113 kb on + strand, within Pf6N2E2_224 at 286.114 kb on - strand, within Pf6N2E2_224 at 286.205 kb on + strand at 286.227 kb on + strand at 286.228 kb on - strand at 286.231 kb on + strand at 286.232 kb on - strand at 286.232 kb on - strand at 286.234 kb on + strand at 286.235 kb on - strand at 286.376 kb on - strand at 286.376 kb on - strand at 286.461 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.865 kb on - strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.617 kb on + strand, within Pf6N2E2_225 at 287.618 kb on - strand, within Pf6N2E2_225 at 287.737 kb on + strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.792 kb on - strand at 287.821 kb on - strand at 287.821 kb on - strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 288.109 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.362 kb on + strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.666 kb on + strand at 288.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 285,423 + Pf6N2E2_224 0.21 -1.3 285,423 + Pf6N2E2_224 0.21 -0.3 285,423 + Pf6N2E2_224 0.21 -1.4 285,424 - Pf6N2E2_224 0.21 +1.0 285,424 - Pf6N2E2_224 0.21 -1.1 285,697 - Pf6N2E2_224 0.48 -2.1 285,748 + Pf6N2E2_224 0.53 -0.7 285,952 + Pf6N2E2_224 0.73 +0.4 286,109 + Pf6N2E2_224 0.89 +0.3 286,109 + Pf6N2E2_224 0.89 +0.9 286,110 - Pf6N2E2_224 0.89 -1.5 286,110 - Pf6N2E2_224 0.89 -1.3 286,110 - Pf6N2E2_224 0.89 -0.7 286,110 - Pf6N2E2_224 0.89 -0.8 286,110 - Pf6N2E2_224 0.89 +0.6 286,111 + Pf6N2E2_224 0.89 +0.6 286,111 + Pf6N2E2_224 0.89 +0.7 286,112 - Pf6N2E2_224 0.89 +0.8 286,112 - Pf6N2E2_224 0.89 +1.3 286,113 + Pf6N2E2_224 0.90 +0.3 286,114 - Pf6N2E2_224 0.90 -0.3 286,205 + +0.4 286,227 + -0.3 286,228 - -0.4 286,231 + -0.6 286,232 - -1.2 286,232 - -0.1 286,234 + +0.5 286,235 - +1.0 286,376 - +0.7 286,376 - +0.3 286,461 - Pf6N2E2_225 0.11 +1.1 286,651 - Pf6N2E2_225 0.23 +1.3 286,651 - Pf6N2E2_225 0.23 +0.8 286,864 + Pf6N2E2_225 0.38 +0.6 286,864 + Pf6N2E2_225 0.38 +0.6 286,865 - Pf6N2E2_225 0.38 +0.9 287,377 + Pf6N2E2_225 0.72 +0.0 287,377 + Pf6N2E2_225 0.72 +1.1 287,377 + Pf6N2E2_225 0.72 +1.2 287,617 + Pf6N2E2_225 0.89 +0.4 287,618 - Pf6N2E2_225 0.89 +0.7 287,737 + -0.9 287,738 - -0.9 287,738 - -0.1 287,738 - +0.2 287,738 - -2.5 287,738 - -1.0 287,792 - +0.2 287,821 - +0.7 287,821 - -0.1 287,898 + +0.9 287,898 + +0.6 287,898 + -0.6 287,899 - +0.4 287,899 - -0.3 287,899 - -3.2 287,899 - +0.2 287,899 - +0.9 288,109 - Pf6N2E2_226 0.30 +0.2 288,256 - Pf6N2E2_226 0.47 +0.4 288,256 - Pf6N2E2_226 0.47 +0.5 288,362 + Pf6N2E2_226 0.59 +1.2 288,363 - Pf6N2E2_226 0.59 +0.4 288,363 - Pf6N2E2_226 0.59 +0.2 288,666 + +0.5 288,730 + -1.9
Or see this region's nucleotide sequence