Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2073 and Pf6N2E2_2074 overlap by 17 nucleotides Pf6N2E2_2074 and Pf6N2E2_2075 are separated by 158 nucleotides
Pf6N2E2_2073 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 2,466,228 to 2,467,976
_2073
Pf6N2E2_2074 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 2,467,960 to 2,468,454
_2074
Pf6N2E2_2075 - Peptidase, U7 family, at 2,468,613 to 2,469,644
_2075
Position (kb)
2467
2468
2469 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2467.026 kb on + strand, within Pf6N2E2_2073 at 2467.026 kb on + strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.209 kb on + strand, within Pf6N2E2_2073 at 2467.317 kb on + strand, within Pf6N2E2_2073 at 2467.648 kb on - strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.879 kb on - strand at 2467.892 kb on + strand at 2467.892 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.959 kb on + strand at 2467.959 kb on + strand at 2467.960 kb on - strand at 2468.314 kb on + strand, within Pf6N2E2_2074 at 2468.315 kb on - strand, within Pf6N2E2_2074 at 2468.407 kb on + strand at 2468.407 kb on + strand at 2468.408 kb on - strand at 2468.603 kb on - strand at 2468.603 kb on - strand at 2468.615 kb on - strand at 2468.615 kb on - strand at 2468.723 kb on - strand, within Pf6N2E2_2075 at 2468.735 kb on - strand, within Pf6N2E2_2075 at 2468.873 kb on - strand, within Pf6N2E2_2075 at 2469.214 kb on + strand, within Pf6N2E2_2075 at 2469.215 kb on - strand, within Pf6N2E2_2075 at 2469.255 kb on + strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,467,026 + Pf6N2E2_2073 0.46 +1.4 2,467,026 + Pf6N2E2_2073 0.46 +1.2 2,467,027 - Pf6N2E2_2073 0.46 +2.0 2,467,027 - Pf6N2E2_2073 0.46 +1.1 2,467,027 - Pf6N2E2_2073 0.46 -0.7 2,467,209 + Pf6N2E2_2073 0.56 +0.5 2,467,317 + Pf6N2E2_2073 0.62 +0.9 2,467,648 - Pf6N2E2_2073 0.81 -1.0 2,467,764 + Pf6N2E2_2073 0.88 +0.5 2,467,764 + Pf6N2E2_2073 0.88 -0.3 2,467,878 + -0.4 2,467,878 + -0.1 2,467,878 + +0.4 2,467,879 - +1.1 2,467,892 + -1.1 2,467,892 + +0.2 2,467,920 + -0.2 2,467,920 + +1.5 2,467,920 + -0.7 2,467,921 - -0.6 2,467,921 - +0.0 2,467,921 - -0.5 2,467,928 + +0.4 2,467,928 + -3.2 2,467,928 + +0.2 2,467,928 + +0.7 2,467,959 + +0.1 2,467,959 + +1.3 2,467,960 - +0.3 2,468,314 + Pf6N2E2_2074 0.72 -3.4 2,468,315 - Pf6N2E2_2074 0.72 -0.7 2,468,407 + -1.6 2,468,407 + +0.4 2,468,408 - +1.2 2,468,603 - +0.2 2,468,603 - -1.8 2,468,615 - -1.3 2,468,615 - -1.6 2,468,723 - Pf6N2E2_2075 0.11 +0.5 2,468,735 - Pf6N2E2_2075 0.12 +0.3 2,468,873 - Pf6N2E2_2075 0.25 -1.4 2,469,214 + Pf6N2E2_2075 0.58 +0.2 2,469,215 - Pf6N2E2_2075 0.58 +0.2 2,469,255 + Pf6N2E2_2075 0.62 +1.4 2,469,256 - Pf6N2E2_2075 0.62 +1.0 2,469,256 - Pf6N2E2_2075 0.62 +0.9 2,469,256 - Pf6N2E2_2075 0.62 +0.3 2,469,256 - Pf6N2E2_2075 0.62 -0.2 2,469,256 - Pf6N2E2_2075 0.62 +1.1 2,469,256 - Pf6N2E2_2075 0.62 +0.1 2,469,256 - Pf6N2E2_2075 0.62 -0.3
Or see this region's nucleotide sequence