Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2021 and Pf6N2E2_2022 are separated by 68 nucleotides Pf6N2E2_2022 and Pf6N2E2_2023 are separated by 184 nucleotides Pf6N2E2_2023 and Pf6N2E2_2024 are separated by 7 nucleotides
Pf6N2E2_2021 - Excinuclease ABC subunit C, at 2,409,570 to 2,411,327
_2021
Pf6N2E2_2022 - BarA-associated response regulator UvrY (= GacA = SirA), at 2,411,396 to 2,411,980
_2022
Pf6N2E2_2023 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,412,165 to 2,412,599
_2023
Pf6N2E2_2024 - Hydrolase, carbon-nitrogen family, at 2,412,607 to 2,413,368
_2024
Position (kb)
2411
2412 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.489 kb on + strand, within Pf6N2E2_2021 at 2410.835 kb on - strand, within Pf6N2E2_2021 at 2410.889 kb on - strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.919 kb on - strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.083 kb on + strand, within Pf6N2E2_2021 at 2411.090 kb on - strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.257 kb on + strand at 2411.258 kb on - strand at 2411.299 kb on + strand at 2411.300 kb on - strand at 2411.480 kb on - strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.951 kb on + strand at 2411.951 kb on + strand at 2411.952 kb on - strand at 2411.986 kb on + strand at 2411.986 kb on + strand at 2411.987 kb on - strand at 2411.987 kb on - strand at 2412.032 kb on - strand at 2412.083 kb on - strand at 2412.106 kb on - strand at 2412.122 kb on + strand at 2412.122 kb on + strand at 2412.123 kb on - strand at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.239 kb on - strand, within Pf6N2E2_2023 at 2412.366 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.521 kb on - strand, within Pf6N2E2_2023 at 2412.812 kb on + strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.842 kb on + strand, within Pf6N2E2_2024 at 2412.842 kb on + strand, within Pf6N2E2_2024 at 2412.875 kb on + strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.882 kb on + strand, within Pf6N2E2_2024
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,410,486 + Pf6N2E2_2021 0.52 +0.3 2,410,486 + Pf6N2E2_2021 0.52 +0.3 2,410,487 - Pf6N2E2_2021 0.52 -1.3 2,410,487 - Pf6N2E2_2021 0.52 -1.4 2,410,487 - Pf6N2E2_2021 0.52 -0.7 2,410,489 + Pf6N2E2_2021 0.52 +1.5 2,410,835 - Pf6N2E2_2021 0.72 +0.4 2,410,889 - Pf6N2E2_2021 0.75 +1.3 2,410,918 + Pf6N2E2_2021 0.77 -3.5 2,410,918 + Pf6N2E2_2021 0.77 +0.3 2,410,919 - Pf6N2E2_2021 0.77 -1.4 2,410,921 + Pf6N2E2_2021 0.77 -1.6 2,410,921 + Pf6N2E2_2021 0.77 +0.2 2,410,921 + Pf6N2E2_2021 0.77 -1.2 2,410,922 - Pf6N2E2_2021 0.77 +0.2 2,410,922 - Pf6N2E2_2021 0.77 -1.9 2,411,011 + Pf6N2E2_2021 0.82 -0.5 2,411,011 + Pf6N2E2_2021 0.82 +0.8 2,411,083 + Pf6N2E2_2021 0.86 -1.7 2,411,090 - Pf6N2E2_2021 0.86 -1.5 2,411,119 + Pf6N2E2_2021 0.88 +0.3 2,411,119 + Pf6N2E2_2021 0.88 -1.2 2,411,257 + -1.5 2,411,258 - -2.4 2,411,299 + +1.1 2,411,300 - +0.8 2,411,480 - Pf6N2E2_2022 0.14 -2.4 2,411,490 + Pf6N2E2_2022 0.16 -0.9 2,411,490 + Pf6N2E2_2022 0.16 -1.2 2,411,491 - Pf6N2E2_2022 0.16 -0.8 2,411,491 - Pf6N2E2_2022 0.16 -0.7 2,411,491 - Pf6N2E2_2022 0.16 -0.7 2,411,491 - Pf6N2E2_2022 0.16 -0.9 2,411,540 + Pf6N2E2_2022 0.25 -1.0 2,411,540 + Pf6N2E2_2022 0.25 -0.9 2,411,541 - Pf6N2E2_2022 0.25 -1.0 2,411,541 - Pf6N2E2_2022 0.25 -0.5 2,411,541 - Pf6N2E2_2022 0.25 -0.3 2,411,667 + Pf6N2E2_2022 0.46 -0.7 2,411,667 + Pf6N2E2_2022 0.46 -0.9 2,411,667 + Pf6N2E2_2022 0.46 -1.3 2,411,667 + Pf6N2E2_2022 0.46 -2.3 2,411,667 + Pf6N2E2_2022 0.46 -0.9 2,411,668 - Pf6N2E2_2022 0.46 -3.6 2,411,668 - Pf6N2E2_2022 0.46 -0.5 2,411,951 + -2.5 2,411,951 + -0.3 2,411,952 - -2.1 2,411,986 + -1.2 2,411,986 + -2.8 2,411,987 - -1.3 2,411,987 - +0.5 2,412,032 - -0.9 2,412,083 - -0.7 2,412,106 - -0.3 2,412,122 + -0.1 2,412,122 + -1.8 2,412,123 - +0.7 2,412,238 + Pf6N2E2_2023 0.17 +1.0 2,412,238 + Pf6N2E2_2023 0.17 -0.3 2,412,238 + Pf6N2E2_2023 0.17 +0.1 2,412,239 - Pf6N2E2_2023 0.17 +0.3 2,412,366 + Pf6N2E2_2023 0.46 -0.1 2,412,385 + Pf6N2E2_2023 0.51 -1.3 2,412,385 + Pf6N2E2_2023 0.51 -1.6 2,412,385 + Pf6N2E2_2023 0.51 -0.4 2,412,385 + Pf6N2E2_2023 0.51 +0.7 2,412,385 + Pf6N2E2_2023 0.51 -0.5 2,412,386 - Pf6N2E2_2023 0.51 +0.4 2,412,386 - Pf6N2E2_2023 0.51 +0.4 2,412,386 - Pf6N2E2_2023 0.51 -2.7 2,412,386 - Pf6N2E2_2023 0.51 +0.4 2,412,386 - Pf6N2E2_2023 0.51 -0.1 2,412,386 - Pf6N2E2_2023 0.51 +0.7 2,412,520 + Pf6N2E2_2023 0.82 -0.3 2,412,520 + Pf6N2E2_2023 0.82 -0.5 2,412,520 + Pf6N2E2_2023 0.82 +0.2 2,412,521 - Pf6N2E2_2023 0.82 +0.1 2,412,812 + Pf6N2E2_2024 0.27 +0.1 2,412,813 - Pf6N2E2_2024 0.27 +0.4 2,412,813 - Pf6N2E2_2024 0.27 +0.8 2,412,813 - Pf6N2E2_2024 0.27 +1.1 2,412,842 + Pf6N2E2_2024 0.31 -0.8 2,412,842 + Pf6N2E2_2024 0.31 -1.4 2,412,875 + Pf6N2E2_2024 0.35 -0.4 2,412,876 - Pf6N2E2_2024 0.35 -0.2 2,412,876 - Pf6N2E2_2024 0.35 -0.7 2,412,876 - Pf6N2E2_2024 0.35 -0.4 2,412,882 + Pf6N2E2_2024 0.36 +0.5
Or see this region's nucleotide sequence