Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotides Pf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides Pf6N2E2_1835 and Pf6N2E2_1836 are separated by 170 nucleotides
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441
_1834
Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049
_1835
Pf6N2E2_1836 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,174,220 to 2,175,422
_1836
Position (kb)
2172
2173
2174 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.506 kb on + strand, within Pf6N2E2_1833 at 2172.574 kb on + strand at 2172.575 kb on - strand at 2172.575 kb on - strand at 2172.663 kb on - strand at 2172.668 kb on + strand at 2172.668 kb on + strand at 2172.669 kb on - strand at 2172.669 kb on - strand at 2172.672 kb on + strand at 2172.673 kb on - strand at 2173.007 kb on + strand, within Pf6N2E2_1834 at 2173.239 kb on - strand, within Pf6N2E2_1834 at 2173.399 kb on + strand at 2173.442 kb on - strand at 2173.442 kb on - strand at 2173.448 kb on + strand at 2173.675 kb on + strand, within Pf6N2E2_1835 at 2173.676 kb on - strand, within Pf6N2E2_1835 at 2173.726 kb on + strand, within Pf6N2E2_1835 at 2173.730 kb on + strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.784 kb on - strand, within Pf6N2E2_1835 at 2173.927 kb on + strand, within Pf6N2E2_1835 at 2174.131 kb on + strand at 2174.132 kb on - strand at 2174.132 kb on - strand at 2174.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 2,171,926 + Pf6N2E2_1833 0.51 +0.6 2,171,926 + Pf6N2E2_1833 0.51 +0.1 2,172,079 + Pf6N2E2_1833 0.61 -2.3 2,172,081 + Pf6N2E2_1833 0.61 +1.1 2,172,156 + Pf6N2E2_1833 0.66 +0.2 2,172,156 + Pf6N2E2_1833 0.66 +0.5 2,172,317 + Pf6N2E2_1833 0.77 -0.2 2,172,317 + Pf6N2E2_1833 0.77 -1.7 2,172,318 - Pf6N2E2_1833 0.77 -0.5 2,172,318 - Pf6N2E2_1833 0.77 +0.9 2,172,506 + Pf6N2E2_1833 0.89 +1.1 2,172,574 + +0.4 2,172,575 - -0.7 2,172,575 - +1.5 2,172,663 - +0.4 2,172,668 + -1.7 2,172,668 + -2.0 2,172,669 - +0.4 2,172,669 - -0.5 2,172,672 + +0.7 2,172,673 - -0.2 2,173,007 + Pf6N2E2_1834 0.44 -0.7 2,173,239 - Pf6N2E2_1834 0.74 -3.6 2,173,399 + +0.4 2,173,442 - +1.3 2,173,442 - +0.7 2,173,448 + +1.5 2,173,675 + Pf6N2E2_1835 0.39 -1.5 2,173,676 - Pf6N2E2_1835 0.39 +0.1 2,173,726 + Pf6N2E2_1835 0.47 +0.4 2,173,730 + Pf6N2E2_1835 0.48 -0.6 2,173,731 - Pf6N2E2_1835 0.48 +0.4 2,173,731 - Pf6N2E2_1835 0.48 -0.6 2,173,731 - Pf6N2E2_1835 0.48 +0.2 2,173,783 + Pf6N2E2_1835 0.56 -1.1 2,173,783 + Pf6N2E2_1835 0.56 -2.9 2,173,784 - Pf6N2E2_1835 0.57 +0.6 2,173,927 + Pf6N2E2_1835 0.80 +0.6 2,174,131 + -0.8 2,174,132 - +0.9 2,174,132 - +0.6 2,174,340 + +0.5
Or see this region's nucleotide sequence