Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_177 and Pf6N2E2_178 are separated by 35 nucleotides Pf6N2E2_178 and Pf6N2E2_179 are separated by 3 nucleotides Pf6N2E2_179 and Pf6N2E2_180 overlap by 20 nucleotides Pf6N2E2_180 and Pf6N2E2_6104 are separated by 90 nucleotides
Pf6N2E2_177 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 226,871 to 227,887
_177
Pf6N2E2_178 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 227,923 to 230,301
_178
Pf6N2E2_179 - Integration host factor alpha subunit, at 230,305 to 230,607
_179
Pf6N2E2_180 - Transcriptional regulator, MerR family, at 230,588 to 230,944
_180
Pf6N2E2_6104 - tRNA-Pro-GGG, at 231,035 to 231,108
_6104
Position (kb) 227
228
229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 229.328 kb on - strand, within Pf6N2E2_178 at 230.304 kb on + strand at 230.304 kb on + strand at 230.304 kb on + strand at 230.305 kb on - strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.588 kb on - strand at 230.588 kb on - strand at 230.605 kb on + strand at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.804 kb on + strand, within Pf6N2E2_180 at 230.869 kb on + strand, within Pf6N2E2_180 at 230.870 kb on - strand, within Pf6N2E2_180 at 231.128 kb on - strand at 231.218 kb on - strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 229,328 - Pf6N2E2_178 0.59 -1.3 230,304 + +3.7 230,304 + +3.6 230,304 + +3.1 230,305 - +3.7 230,587 + +0.9 230,587 + -0.7 230,587 + +0.7 230,587 + +0.2 230,587 + +1.9 230,588 - +3.1 230,588 - +1.7 230,605 + +1.5 230,756 + Pf6N2E2_180 0.47 +0.3 230,756 + Pf6N2E2_180 0.47 +0.5 230,756 + Pf6N2E2_180 0.47 -0.5 230,804 + Pf6N2E2_180 0.61 -0.4 230,869 + Pf6N2E2_180 0.79 -2.0 230,870 - Pf6N2E2_180 0.79 +0.1 231,128 - +1.6 231,218 - +0.2 231,283 + -0.2 231,283 + -2.4 231,283 + -0.6 231,283 + -1.6 231,283 + -1.4 231,283 + +2.0 231,284 - -1.5
Or see this region's nucleotide sequence