Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1666 and Pf6N2E2_1667 are separated by 113 nucleotides Pf6N2E2_1667 and Pf6N2E2_1668 are separated by 43 nucleotides
Pf6N2E2_1666 - Transcriptional regulator, LysR family, at 1,972,324 to 1,973,262
_1666
Pf6N2E2_1667 - Fumarylacetoacetate hydrolase family protein, at 1,973,376 to 1,974,557
_1667
Pf6N2E2_1668 - Xylonate dehydratase (EC 4.2.1.82) (from data) , at 1,974,601 to 1,976,385
_1668
Position (kb)
1973
1974
1975 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1972.709 kb on + strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.839 kb on + strand, within Pf6N2E2_1666 at 1973.028 kb on - strand, within Pf6N2E2_1666 at 1973.035 kb on + strand, within Pf6N2E2_1666 at 1973.118 kb on + strand, within Pf6N2E2_1666 at 1973.266 kb on + strand at 1973.312 kb on - strand at 1973.342 kb on + strand at 1973.343 kb on - strand at 1973.356 kb on + strand at 1973.390 kb on + strand at 1973.390 kb on + strand at 1973.390 kb on + strand at 1973.390 kb on + strand at 1973.390 kb on + strand at 1973.391 kb on - strand at 1973.391 kb on - strand at 1973.391 kb on - strand at 1973.402 kb on + strand at 1973.403 kb on - strand at 1973.503 kb on + strand, within Pf6N2E2_1667 at 1973.504 kb on - strand, within Pf6N2E2_1667 at 1973.504 kb on - strand, within Pf6N2E2_1667 at 1973.865 kb on - strand, within Pf6N2E2_1667 at 1974.224 kb on - strand, within Pf6N2E2_1667 at 1974.525 kb on - strand at 1974.582 kb on + strand at 1974.583 kb on - strand at 1974.583 kb on - strand at 1974.679 kb on + strand at 1974.691 kb on + strand at 1974.700 kb on + strand at 1974.701 kb on - strand at 1974.701 kb on - strand at 1974.919 kb on + strand, within Pf6N2E2_1668 at 1974.920 kb on - strand, within Pf6N2E2_1668 at 1974.920 kb on - strand, within Pf6N2E2_1668 at 1974.949 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.206 kb on + strand, within Pf6N2E2_1668 at 1975.207 kb on - strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.316 kb on - strand, within Pf6N2E2_1668 at 1975.556 kb on - strand, within Pf6N2E2_1668 at 1975.556 kb on - strand, within Pf6N2E2_1668
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,972,709 + Pf6N2E2_1666 0.41 -1.5 1,972,710 - Pf6N2E2_1666 0.41 +0.2 1,972,710 - Pf6N2E2_1666 0.41 +0.7 1,972,839 + Pf6N2E2_1666 0.55 +1.3 1,973,028 - Pf6N2E2_1666 0.75 +1.2 1,973,035 + Pf6N2E2_1666 0.76 -1.6 1,973,118 + Pf6N2E2_1666 0.85 +0.8 1,973,266 + +2.6 1,973,312 - +0.4 1,973,342 + -0.2 1,973,343 - -0.6 1,973,356 + -0.6 1,973,390 + -1.5 1,973,390 + +0.7 1,973,390 + +0.2 1,973,390 + -1.8 1,973,390 + +0.9 1,973,391 - -0.3 1,973,391 - +1.6 1,973,391 - +0.0 1,973,402 + -0.5 1,973,403 - +0.1 1,973,503 + Pf6N2E2_1667 0.11 -0.2 1,973,504 - Pf6N2E2_1667 0.11 +1.4 1,973,504 - Pf6N2E2_1667 0.11 -0.6 1,973,865 - Pf6N2E2_1667 0.41 -1.1 1,974,224 - Pf6N2E2_1667 0.72 -2.4 1,974,525 - +0.1 1,974,582 + -1.4 1,974,583 - -1.8 1,974,583 - -1.8 1,974,679 + -0.8 1,974,691 + +0.5 1,974,700 + +0.0 1,974,701 - -0.1 1,974,701 - -1.5 1,974,919 + Pf6N2E2_1668 0.18 -0.5 1,974,920 - Pf6N2E2_1668 0.18 -0.1 1,974,920 - Pf6N2E2_1668 0.18 +0.1 1,974,949 + Pf6N2E2_1668 0.19 +1.3 1,975,106 + Pf6N2E2_1668 0.28 +0.3 1,975,106 + Pf6N2E2_1668 0.28 +1.2 1,975,106 + Pf6N2E2_1668 0.28 +0.5 1,975,106 + Pf6N2E2_1668 0.28 -1.1 1,975,206 + Pf6N2E2_1668 0.34 +0.6 1,975,207 - Pf6N2E2_1668 0.34 -0.1 1,975,315 + Pf6N2E2_1668 0.40 +0.9 1,975,315 + Pf6N2E2_1668 0.40 +0.5 1,975,315 + Pf6N2E2_1668 0.40 -0.9 1,975,316 - Pf6N2E2_1668 0.40 +0.8 1,975,556 - Pf6N2E2_1668 0.54 -0.4 1,975,556 - Pf6N2E2_1668 0.54 +0.5
Or see this region's nucleotide sequence