Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotides Pf6N2E2_1625 and Pf6N2E2_1626 are separated by 7 nucleotides Pf6N2E2_1626 and Pf6N2E2_1627 overlap by 4 nucleotides
Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709
_1624
Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650
_1625
Pf6N2E2_1626 - Putative ATP-binding component of a transport system, at 1,901,658 to 1,903,301
_1626
Pf6N2E2_1627 - Non-ribosomal peptide synthase, at 1,903,298 to 1,907,083
_1627
Position (kb)
1901
1902
1903
1904 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.751 kb on - strand at 1900.855 kb on - strand at 1900.855 kb on - strand at 1900.856 kb on + strand at 1900.856 kb on + strand at 1900.857 kb on - strand at 1900.857 kb on - strand at 1901.001 kb on + strand, within Pf6N2E2_1625 at 1901.031 kb on - strand, within Pf6N2E2_1625 at 1901.291 kb on - strand, within Pf6N2E2_1625 at 1901.383 kb on + strand, within Pf6N2E2_1625 at 1901.384 kb on - strand, within Pf6N2E2_1625 at 1901.648 kb on + strand at 1901.648 kb on + strand at 1901.659 kb on + strand at 1901.698 kb on + strand at 1901.716 kb on + strand at 1901.729 kb on - strand at 1901.753 kb on - strand at 1901.821 kb on + strand at 1901.821 kb on + strand at 1901.822 kb on - strand at 1901.905 kb on - strand, within Pf6N2E2_1626 at 1902.172 kb on + strand, within Pf6N2E2_1626 at 1902.172 kb on + strand, within Pf6N2E2_1626 at 1902.173 kb on - strand, within Pf6N2E2_1626 at 1902.173 kb on - strand, within Pf6N2E2_1626 at 1902.499 kb on + strand, within Pf6N2E2_1626 at 1902.499 kb on + strand, within Pf6N2E2_1626 at 1902.558 kb on - strand, within Pf6N2E2_1626 at 1902.567 kb on + strand, within Pf6N2E2_1626 at 1902.880 kb on + strand, within Pf6N2E2_1626 at 1902.913 kb on + strand, within Pf6N2E2_1626 at 1902.913 kb on + strand, within Pf6N2E2_1626 at 1902.914 kb on - strand, within Pf6N2E2_1626 at 1902.980 kb on - strand, within Pf6N2E2_1626 at 1902.980 kb on - strand, within Pf6N2E2_1626 at 1902.984 kb on - strand, within Pf6N2E2_1626 at 1903.122 kb on + strand, within Pf6N2E2_1626 at 1903.122 kb on + strand, within Pf6N2E2_1626 at 1903.123 kb on - strand, within Pf6N2E2_1626 at 1903.196 kb on + strand at 1903.197 kb on - strand at 1903.197 kb on - strand at 1903.337 kb on + strand at 1903.338 kb on - strand at 1903.515 kb on + strand at 1903.638 kb on + strand at 1903.639 kb on - strand at 1903.713 kb on + strand, within Pf6N2E2_1627 at 1903.924 kb on - strand, within Pf6N2E2_1627 at 1903.924 kb on - strand, within Pf6N2E2_1627 at 1903.924 kb on - strand, within Pf6N2E2_1627 at 1903.924 kb on - strand, within Pf6N2E2_1627 at 1903.924 kb on - strand, within Pf6N2E2_1627 at 1904.006 kb on + strand, within Pf6N2E2_1627 at 1904.016 kb on - strand, within Pf6N2E2_1627 at 1904.016 kb on - strand, within Pf6N2E2_1627 at 1904.028 kb on + strand, within Pf6N2E2_1627 at 1904.159 kb on - strand, within Pf6N2E2_1627
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,900,672 - Pf6N2E2_1624 0.82 +0.9 1,900,672 - Pf6N2E2_1624 0.82 -0.1 1,900,751 - -0.6 1,900,855 - +0.5 1,900,855 - +1.5 1,900,856 + +1.5 1,900,856 + -1.1 1,900,857 - +2.3 1,900,857 - -1.3 1,901,001 + Pf6N2E2_1625 0.12 +2.5 1,901,031 - Pf6N2E2_1625 0.16 +3.6 1,901,291 - Pf6N2E2_1625 0.51 +2.8 1,901,383 + Pf6N2E2_1625 0.64 +3.5 1,901,384 - Pf6N2E2_1625 0.64 +0.3 1,901,648 + +1.6 1,901,648 + -0.1 1,901,659 + +1.3 1,901,698 + +2.9 1,901,716 + +2.5 1,901,729 - +3.8 1,901,753 - +3.1 1,901,821 + +1.0 1,901,821 + +2.4 1,901,822 - +3.1 1,901,905 - Pf6N2E2_1626 0.15 +0.7 1,902,172 + Pf6N2E2_1626 0.31 +2.3 1,902,172 + Pf6N2E2_1626 0.31 +2.0 1,902,173 - Pf6N2E2_1626 0.31 +3.6 1,902,173 - Pf6N2E2_1626 0.31 +4.1 1,902,499 + Pf6N2E2_1626 0.51 +3.2 1,902,499 + Pf6N2E2_1626 0.51 +2.3 1,902,558 - Pf6N2E2_1626 0.55 +1.9 1,902,567 + Pf6N2E2_1626 0.55 +2.1 1,902,880 + Pf6N2E2_1626 0.74 +2.5 1,902,913 + Pf6N2E2_1626 0.76 +3.6 1,902,913 + Pf6N2E2_1626 0.76 +4.1 1,902,914 - Pf6N2E2_1626 0.76 +2.3 1,902,980 - Pf6N2E2_1626 0.80 +3.5 1,902,980 - Pf6N2E2_1626 0.80 +1.5 1,902,984 - Pf6N2E2_1626 0.81 +2.5 1,903,122 + Pf6N2E2_1626 0.89 +2.0 1,903,122 + Pf6N2E2_1626 0.89 +0.2 1,903,123 - Pf6N2E2_1626 0.89 +3.0 1,903,196 + +2.5 1,903,197 - +3.5 1,903,197 - +2.6 1,903,337 + +1.0 1,903,338 - +0.2 1,903,515 + +3.0 1,903,638 + +1.4 1,903,639 - +0.6 1,903,713 + Pf6N2E2_1627 0.11 +1.5 1,903,924 - Pf6N2E2_1627 0.17 -0.3 1,903,924 - Pf6N2E2_1627 0.17 +1.3 1,903,924 - Pf6N2E2_1627 0.17 -0.4 1,903,924 - Pf6N2E2_1627 0.17 -0.1 1,903,924 - Pf6N2E2_1627 0.17 +0.3 1,904,006 + Pf6N2E2_1627 0.19 +2.0 1,904,016 - Pf6N2E2_1627 0.19 -1.0 1,904,016 - Pf6N2E2_1627 0.19 +0.7 1,904,028 + Pf6N2E2_1627 0.19 +1.2 1,904,159 - Pf6N2E2_1627 0.23 +0.7
Or see this region's nucleotide sequence