Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1621 and Pf6N2E2_1622 are separated by 441 nucleotides Pf6N2E2_1622 and Pf6N2E2_1623 are separated by 157 nucleotides
Pf6N2E2_1621 - hypothetical protein, at 1,895,396 to 1,896,457
_1621
Pf6N2E2_1622 - Methyl-accepting chemotaxis protein, at 1,896,899 to 1,898,869
_1622
Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457
_1623
Position (kb)
1896
1897
1898
1899 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1896.033 kb on + strand, within Pf6N2E2_1621 at 1896.034 kb on - strand, within Pf6N2E2_1621 at 1896.034 kb on - strand, within Pf6N2E2_1621 at 1896.034 kb on - strand, within Pf6N2E2_1621 at 1896.034 kb on - strand, within Pf6N2E2_1621 at 1896.034 kb on - strand, within Pf6N2E2_1621 at 1896.225 kb on + strand, within Pf6N2E2_1621 at 1896.226 kb on - strand, within Pf6N2E2_1621 at 1896.286 kb on - strand, within Pf6N2E2_1621 at 1896.286 kb on - strand, within Pf6N2E2_1621 at 1896.365 kb on - strand at 1896.366 kb on + strand at 1896.378 kb on + strand at 1896.492 kb on + strand at 1896.492 kb on + strand at 1896.493 kb on - strand at 1896.493 kb on - strand at 1896.493 kb on - strand at 1896.493 kb on - strand at 1896.496 kb on + strand at 1896.496 kb on + strand at 1896.496 kb on + strand at 1896.497 kb on - strand at 1896.572 kb on - strand at 1896.755 kb on + strand at 1896.756 kb on - strand at 1896.803 kb on - strand at 1896.803 kb on - strand at 1896.849 kb on + strand at 1896.849 kb on + strand at 1896.849 kb on + strand at 1896.850 kb on - strand at 1896.850 kb on - strand at 1896.894 kb on + strand at 1896.894 kb on + strand at 1896.942 kb on + strand at 1896.942 kb on + strand at 1896.942 kb on + strand at 1896.942 kb on + strand at 1896.942 kb on + strand at 1896.942 kb on + strand at 1896.943 kb on - strand at 1896.943 kb on - strand at 1896.943 kb on - strand at 1896.946 kb on + strand at 1896.946 kb on + strand at 1896.947 kb on - strand at 1897.091 kb on + strand at 1897.091 kb on + strand at 1897.092 kb on - strand at 1897.253 kb on - strand, within Pf6N2E2_1622 at 1897.307 kb on + strand, within Pf6N2E2_1622 at 1897.348 kb on + strand, within Pf6N2E2_1622 at 1897.361 kb on + strand, within Pf6N2E2_1622 at 1897.361 kb on + strand, within Pf6N2E2_1622 at 1897.361 kb on + strand, within Pf6N2E2_1622 at 1897.961 kb on - strand, within Pf6N2E2_1622 at 1898.087 kb on + strand, within Pf6N2E2_1622 at 1898.088 kb on - strand, within Pf6N2E2_1622 at 1898.111 kb on + strand, within Pf6N2E2_1622 at 1898.112 kb on - strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.293 kb on - strand, within Pf6N2E2_1622 at 1898.345 kb on + strand, within Pf6N2E2_1622 at 1898.358 kb on + strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.518 kb on - strand, within Pf6N2E2_1622 at 1899.036 kb on - strand at 1899.307 kb on - strand, within Pf6N2E2_1623 at 1899.489 kb on - strand, within Pf6N2E2_1623 at 1899.552 kb on + strand, within Pf6N2E2_1623 at 1899.553 kb on - strand, within Pf6N2E2_1623 at 1899.691 kb on - strand, within Pf6N2E2_1623
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,896,033 + Pf6N2E2_1621 0.60 +1.3 1,896,034 - Pf6N2E2_1621 0.60 +1.0 1,896,034 - Pf6N2E2_1621 0.60 -0.6 1,896,034 - Pf6N2E2_1621 0.60 +1.2 1,896,034 - Pf6N2E2_1621 0.60 -0.0 1,896,034 - Pf6N2E2_1621 0.60 -1.2 1,896,225 + Pf6N2E2_1621 0.78 +0.7 1,896,226 - Pf6N2E2_1621 0.78 +0.6 1,896,286 - Pf6N2E2_1621 0.84 +0.7 1,896,286 - Pf6N2E2_1621 0.84 -0.1 1,896,365 - +0.3 1,896,366 + -0.1 1,896,378 + +2.1 1,896,492 + +1.3 1,896,492 + -2.4 1,896,493 - -0.6 1,896,493 - -0.3 1,896,493 - +1.2 1,896,493 - +1.5 1,896,496 + +1.6 1,896,496 + +0.6 1,896,496 + +0.6 1,896,497 - -1.8 1,896,572 - +0.2 1,896,755 + +0.7 1,896,756 - +0.2 1,896,803 - +0.6 1,896,803 - +0.3 1,896,849 + -0.5 1,896,849 + +1.1 1,896,849 + +0.3 1,896,850 - -2.1 1,896,850 - -0.2 1,896,894 + +0.4 1,896,894 + +0.2 1,896,942 + +0.3 1,896,942 + +0.5 1,896,942 + +1.6 1,896,942 + +0.9 1,896,942 + +0.7 1,896,942 + +3.1 1,896,943 - +0.6 1,896,943 - +1.5 1,896,943 - +0.6 1,896,946 + +0.9 1,896,946 + -0.1 1,896,947 - -0.0 1,897,091 + +1.1 1,897,091 + -0.6 1,897,092 - +0.9 1,897,253 - Pf6N2E2_1622 0.18 +0.1 1,897,307 + Pf6N2E2_1622 0.21 +0.5 1,897,348 + Pf6N2E2_1622 0.23 -0.4 1,897,361 + Pf6N2E2_1622 0.23 +0.9 1,897,361 + Pf6N2E2_1622 0.23 +0.8 1,897,361 + Pf6N2E2_1622 0.23 -0.0 1,897,961 - Pf6N2E2_1622 0.54 +0.9 1,898,087 + Pf6N2E2_1622 0.60 +1.4 1,898,088 - Pf6N2E2_1622 0.60 +0.0 1,898,111 + Pf6N2E2_1622 0.61 -2.4 1,898,112 - Pf6N2E2_1622 0.62 +0.4 1,898,292 + Pf6N2E2_1622 0.71 +0.7 1,898,292 + Pf6N2E2_1622 0.71 -0.3 1,898,293 - Pf6N2E2_1622 0.71 -0.0 1,898,345 + Pf6N2E2_1622 0.73 +0.5 1,898,358 + Pf6N2E2_1622 0.74 -0.9 1,898,359 - Pf6N2E2_1622 0.74 +0.7 1,898,359 - Pf6N2E2_1622 0.74 -1.8 1,898,359 - Pf6N2E2_1622 0.74 -0.1 1,898,518 - Pf6N2E2_1622 0.82 +0.4 1,899,036 - +0.2 1,899,307 - Pf6N2E2_1623 0.20 +0.5 1,899,489 - Pf6N2E2_1623 0.32 +0.1 1,899,552 + Pf6N2E2_1623 0.37 +0.7 1,899,553 - Pf6N2E2_1623 0.37 +0.1 1,899,691 - Pf6N2E2_1623 0.46 -1.7
Or see this region's nucleotide sequence