Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1611

Experiment: Phosphomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1609 and Pf6N2E2_1610 overlap by 4 nucleotidesPf6N2E2_1610 and Pf6N2E2_1611 overlap by 7 nucleotidesPf6N2E2_1611 and Pf6N2E2_1612 overlap by 4 nucleotidesPf6N2E2_1612 and Pf6N2E2_1613 are separated by 186 nucleotides Pf6N2E2_1609 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,883,343 to 1,884,194 _1609 Pf6N2E2_1610 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 1,884,191 to 1,884,988 _1610 Pf6N2E2_1611 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 1,884,982 to 1,885,809 _1611 Pf6N2E2_1612 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 1,885,806 to 1,886,573 _1612 Pf6N2E2_1613 - short-chain dehydrogenase/reductase, at 1,886,760 to 1,887,503 _1613 Position (kb) 1884 1885 1886Strain fitness (log2 ratio) -2 -1 0 1at 1883.988 kb on + strand, within Pf6N2E2_1609at 1883.988 kb on + strand, within Pf6N2E2_1609at 1883.988 kb on + strand, within Pf6N2E2_1609at 1883.988 kb on + strand, within Pf6N2E2_1609at 1883.998 kb on - strand, within Pf6N2E2_1609at 1883.998 kb on - strand, within Pf6N2E2_1609at 1884.047 kb on - strand, within Pf6N2E2_1609at 1884.047 kb on - strand, within Pf6N2E2_1609at 1884.147 kb on + strandat 1884.147 kb on + strandat 1884.147 kb on + strandat 1884.147 kb on + strandat 1884.147 kb on + strandat 1884.148 kb on - strandat 1884.148 kb on - strandat 1884.148 kb on - strandat 1884.148 kb on - strandat 1884.917 kb on + strandat 1884.933 kb on + strandat 1884.934 kb on - strandat 1884.934 kb on - strandat 1884.934 kb on - strandat 1885.136 kb on + strand, within Pf6N2E2_1611at 1885.458 kb on + strand, within Pf6N2E2_1611at 1885.458 kb on + strand, within Pf6N2E2_1611at 1885.459 kb on - strand, within Pf6N2E2_1611at 1885.498 kb on + strand, within Pf6N2E2_1611at 1885.619 kb on - strand, within Pf6N2E2_1611at 1885.932 kb on + strand, within Pf6N2E2_1612at 1885.933 kb on - strand, within Pf6N2E2_1612at 1886.057 kb on - strand, within Pf6N2E2_1612at 1886.367 kb on + strand, within Pf6N2E2_1612at 1886.367 kb on + strand, within Pf6N2E2_1612at 1886.367 kb on + strand, within Pf6N2E2_1612at 1886.367 kb on + strand, within Pf6N2E2_1612at 1886.368 kb on - strand, within Pf6N2E2_1612at 1886.368 kb on - strand, within Pf6N2E2_1612at 1886.451 kb on + strand, within Pf6N2E2_1612at 1886.451 kb on + strand, within Pf6N2E2_1612at 1886.451 kb on + strand, within Pf6N2E2_1612at 1886.451 kb on + strand, within Pf6N2E2_1612at 1886.609 kb on - strandat 1886.698 kb on + strandat 1886.745 kb on + strandat 1886.766 kb on - strandat 1886.787 kb on - strandat 1886.791 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml
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1,883,988 + Pf6N2E2_1609 0.76 +0.6
1,883,988 + Pf6N2E2_1609 0.76 +0.4
1,883,988 + Pf6N2E2_1609 0.76 +0.9
1,883,988 + Pf6N2E2_1609 0.76 -0.2
1,883,998 - Pf6N2E2_1609 0.77 +0.0
1,883,998 - Pf6N2E2_1609 0.77 -1.1
1,884,047 - Pf6N2E2_1609 0.83 -0.3
1,884,047 - Pf6N2E2_1609 0.83 -0.4
1,884,147 + -0.1
1,884,147 + -2.2
1,884,147 + +0.6
1,884,147 + +1.3
1,884,147 + +0.2
1,884,148 - +0.2
1,884,148 - +0.8
1,884,148 - -0.7
1,884,148 - +0.7
1,884,917 + -1.3
1,884,933 + -0.3
1,884,934 - +0.4
1,884,934 - +0.1
1,884,934 - +1.0
1,885,136 + Pf6N2E2_1611 0.19 +0.1
1,885,458 + Pf6N2E2_1611 0.57 -0.5
1,885,458 + Pf6N2E2_1611 0.57 -0.0
1,885,459 - Pf6N2E2_1611 0.58 -0.9
1,885,498 + Pf6N2E2_1611 0.62 +1.1
1,885,619 - Pf6N2E2_1611 0.77 -0.9
1,885,932 + Pf6N2E2_1612 0.16 -1.8
1,885,933 - Pf6N2E2_1612 0.17 -2.1
1,886,057 - Pf6N2E2_1612 0.33 +0.3
1,886,367 + Pf6N2E2_1612 0.73 +0.9
1,886,367 + Pf6N2E2_1612 0.73 +0.2
1,886,367 + Pf6N2E2_1612 0.73 -0.2
1,886,367 + Pf6N2E2_1612 0.73 +0.3
1,886,368 - Pf6N2E2_1612 0.73 +1.3
1,886,368 - Pf6N2E2_1612 0.73 -1.7
1,886,451 + Pf6N2E2_1612 0.84 -1.5
1,886,451 + Pf6N2E2_1612 0.84 +0.3
1,886,451 + Pf6N2E2_1612 0.84 -1.2
1,886,451 + Pf6N2E2_1612 0.84 +1.0
1,886,609 - +0.2
1,886,698 + +1.1
1,886,745 + -0.7
1,886,766 - -1.3
1,886,787 - -0.3
1,886,791 + -0.6

Or see this region's nucleotide sequence