Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1588 and Pf6N2E2_1589 overlap by 11 nucleotides Pf6N2E2_1589 and Pf6N2E2_1590 are separated by 3 nucleotides
Pf6N2E2_1588 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,857,902 to 1,859,422
_1588
Pf6N2E2_1589 - Phosphoserine phosphatase (EC 3.1.3.3), at 1,859,412 to 1,860,023
_1589
Pf6N2E2_1590 - pentachlorophenol 4-monooxygenase, at 1,860,027 to 1,861,790
_1590
Position (kb)
1859
1860
1861 Strain fitness (log2 ratio)
-2
-1
0
1 at 1858.479 kb on - strand, within Pf6N2E2_1588 at 1858.730 kb on - strand, within Pf6N2E2_1588 at 1858.788 kb on - strand, within Pf6N2E2_1588 at 1858.842 kb on + strand, within Pf6N2E2_1588 at 1858.916 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.049 kb on - strand, within Pf6N2E2_1588 at 1859.252 kb on + strand, within Pf6N2E2_1588 at 1859.253 kb on - strand, within Pf6N2E2_1588 at 1859.351 kb on + strand at 1859.389 kb on + strand at 1859.390 kb on - strand at 1859.504 kb on + strand, within Pf6N2E2_1589 at 1859.619 kb on + strand, within Pf6N2E2_1589 at 1859.664 kb on + strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.814 kb on + strand, within Pf6N2E2_1589 at 1859.815 kb on - strand, within Pf6N2E2_1589 at 1859.885 kb on - strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1860.006 kb on + strand at 1860.006 kb on + strand at 1860.007 kb on - strand at 1860.019 kb on - strand at 1860.327 kb on + strand, within Pf6N2E2_1590 at 1860.340 kb on - strand, within Pf6N2E2_1590 at 1860.606 kb on + strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.756 kb on + strand, within Pf6N2E2_1590 at 1860.757 kb on - strand, within Pf6N2E2_1590 at 1860.941 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.994 kb on - strand, within Pf6N2E2_1590 at 1860.996 kb on - strand, within Pf6N2E2_1590 at 1861.017 kb on + strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,858,479 - Pf6N2E2_1588 0.38 +0.4 1,858,730 - Pf6N2E2_1588 0.54 +1.0 1,858,788 - Pf6N2E2_1588 0.58 +0.5 1,858,842 + Pf6N2E2_1588 0.62 -0.0 1,858,916 - Pf6N2E2_1588 0.67 +1.5 1,859,023 - Pf6N2E2_1588 0.74 +1.3 1,859,023 - Pf6N2E2_1588 0.74 -0.5 1,859,049 - Pf6N2E2_1588 0.75 +0.6 1,859,252 + Pf6N2E2_1588 0.89 +1.2 1,859,253 - Pf6N2E2_1588 0.89 -0.1 1,859,351 + +1.4 1,859,389 + +1.4 1,859,390 - +0.9 1,859,504 + Pf6N2E2_1589 0.15 +0.5 1,859,619 + Pf6N2E2_1589 0.34 +0.2 1,859,664 + Pf6N2E2_1589 0.41 -0.0 1,859,665 - Pf6N2E2_1589 0.41 -0.6 1,859,665 - Pf6N2E2_1589 0.41 +0.5 1,859,665 - Pf6N2E2_1589 0.41 -0.5 1,859,779 - Pf6N2E2_1589 0.60 +0.5 1,859,779 - Pf6N2E2_1589 0.60 +0.4 1,859,814 + Pf6N2E2_1589 0.66 +1.3 1,859,815 - Pf6N2E2_1589 0.66 -1.3 1,859,885 - Pf6N2E2_1589 0.77 -2.4 1,859,946 + Pf6N2E2_1589 0.87 +0.2 1,859,946 + Pf6N2E2_1589 0.87 +0.8 1,859,946 + Pf6N2E2_1589 0.87 +1.2 1,859,947 - Pf6N2E2_1589 0.87 +0.7 1,859,947 - Pf6N2E2_1589 0.87 +1.5 1,859,947 - Pf6N2E2_1589 0.87 -0.3 1,860,006 + -1.2 1,860,006 + +1.0 1,860,007 - -1.2 1,860,019 - -0.3 1,860,327 + Pf6N2E2_1590 0.17 -0.6 1,860,340 - Pf6N2E2_1590 0.18 +0.6 1,860,606 + Pf6N2E2_1590 0.33 +1.0 1,860,697 - Pf6N2E2_1590 0.38 -1.7 1,860,697 - Pf6N2E2_1590 0.38 -0.9 1,860,697 - Pf6N2E2_1590 0.38 +1.6 1,860,697 - Pf6N2E2_1590 0.38 +0.4 1,860,756 + Pf6N2E2_1590 0.41 -2.1 1,860,757 - Pf6N2E2_1590 0.41 +0.4 1,860,941 + Pf6N2E2_1590 0.52 +0.7 1,860,993 + Pf6N2E2_1590 0.55 +0.4 1,860,993 + Pf6N2E2_1590 0.55 +0.0 1,860,993 + Pf6N2E2_1590 0.55 +0.1 1,860,994 - Pf6N2E2_1590 0.55 +0.1 1,860,996 - Pf6N2E2_1590 0.55 +0.6 1,861,017 + Pf6N2E2_1590 0.56 +0.6 1,861,018 - Pf6N2E2_1590 0.56 +0.8 1,861,018 - Pf6N2E2_1590 0.56 +0.5 1,861,018 - Pf6N2E2_1590 0.56 -0.3 1,861,018 - Pf6N2E2_1590 0.56 +0.6 1,861,018 - Pf6N2E2_1590 0.56 -0.2
Or see this region's nucleotide sequence