Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1543 and Pf6N2E2_1544 overlap by 11 nucleotides Pf6N2E2_1544 and Pf6N2E2_1545 are separated by 199 nucleotides Pf6N2E2_1545 and Pf6N2E2_1546 are separated by 164 nucleotides
Pf6N2E2_1543 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,801,238 to 1,802,719
_1543
Pf6N2E2_1544 - Thermostable hemolysin delta-VPH, at 1,802,709 to 1,803,386
_1544
Pf6N2E2_1545 - N-carbamoylputrescine amidase (EC 3.5.1.53), at 1,803,586 to 1,805,337
_1545
Pf6N2E2_1546 - Transcriptional regulator, LysR family, at 1,805,502 to 1,806,356
_1546
Position (kb)
1803
1804
1805
1806 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1802.593 kb on - strand at 1802.607 kb on + strand at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.422 kb on + strand at 1803.423 kb on - strand at 1803.423 kb on - strand at 1803.439 kb on - strand at 1803.523 kb on + strand at 1803.523 kb on + strand at 1803.718 kb on + strand at 1803.872 kb on - strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.890 kb on - strand, within Pf6N2E2_1545 at 1804.087 kb on + strand, within Pf6N2E2_1545 at 1804.228 kb on - strand, within Pf6N2E2_1545 at 1804.498 kb on + strand, within Pf6N2E2_1545 at 1804.698 kb on + strand, within Pf6N2E2_1545 at 1804.698 kb on + strand, within Pf6N2E2_1545 at 1804.699 kb on - strand, within Pf6N2E2_1545 at 1804.703 kb on + strand, within Pf6N2E2_1545 at 1804.907 kb on - strand, within Pf6N2E2_1545 at 1804.907 kb on - strand, within Pf6N2E2_1545 at 1805.169 kb on + strand at 1805.243 kb on - strand at 1805.504 kb on - strand at 1805.515 kb on + strand at 1805.516 kb on - strand at 1805.955 kb on + strand, within Pf6N2E2_1546 at 1805.956 kb on - strand, within Pf6N2E2_1546 at 1806.049 kb on + strand, within Pf6N2E2_1546 at 1806.050 kb on - strand, within Pf6N2E2_1546 at 1806.071 kb on + strand, within Pf6N2E2_1546 at 1806.071 kb on + strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.072 kb on - strand, within Pf6N2E2_1546 at 1806.239 kb on + strand, within Pf6N2E2_1546 at 1806.240 kb on - strand, within Pf6N2E2_1546 at 1806.240 kb on - strand, within Pf6N2E2_1546 at 1806.323 kb on + strand at 1806.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,802,593 - -2.0 1,802,607 + -0.7 1,802,986 + Pf6N2E2_1544 0.41 -0.2 1,802,986 + Pf6N2E2_1544 0.41 -0.6 1,802,986 + Pf6N2E2_1544 0.41 +0.0 1,802,987 - Pf6N2E2_1544 0.41 +0.5 1,802,987 - Pf6N2E2_1544 0.41 -1.8 1,802,987 - Pf6N2E2_1544 0.41 +1.4 1,802,987 - Pf6N2E2_1544 0.41 +1.2 1,802,987 - Pf6N2E2_1544 0.41 -0.3 1,803,121 + Pf6N2E2_1544 0.61 +0.2 1,803,121 + Pf6N2E2_1544 0.61 +0.7 1,803,121 + Pf6N2E2_1544 0.61 -0.1 1,803,422 + +0.8 1,803,423 - +0.9 1,803,423 - +0.9 1,803,439 - +0.5 1,803,523 + -0.1 1,803,523 + +1.7 1,803,718 + +0.1 1,803,872 - Pf6N2E2_1545 0.16 -1.3 1,803,889 + Pf6N2E2_1545 0.17 -1.4 1,803,889 + Pf6N2E2_1545 0.17 -2.1 1,803,889 + Pf6N2E2_1545 0.17 -2.3 1,803,890 - Pf6N2E2_1545 0.17 -1.9 1,804,087 + Pf6N2E2_1545 0.29 -1.8 1,804,228 - Pf6N2E2_1545 0.37 -1.9 1,804,498 + Pf6N2E2_1545 0.52 -0.6 1,804,698 + Pf6N2E2_1545 0.63 -1.3 1,804,698 + Pf6N2E2_1545 0.63 -2.5 1,804,699 - Pf6N2E2_1545 0.64 -1.9 1,804,703 + Pf6N2E2_1545 0.64 -3.0 1,804,907 - Pf6N2E2_1545 0.75 -1.0 1,804,907 - Pf6N2E2_1545 0.75 -3.2 1,805,169 + -1.3 1,805,243 - -0.7 1,805,504 - +0.2 1,805,515 + -0.3 1,805,516 - +0.5 1,805,955 + Pf6N2E2_1546 0.53 +0.3 1,805,956 - Pf6N2E2_1546 0.53 +1.3 1,806,049 + Pf6N2E2_1546 0.64 +0.2 1,806,050 - Pf6N2E2_1546 0.64 +1.6 1,806,071 + Pf6N2E2_1546 0.67 +1.9 1,806,071 + Pf6N2E2_1546 0.67 -1.5 1,806,072 - Pf6N2E2_1546 0.67 +0.0 1,806,072 - Pf6N2E2_1546 0.67 -1.0 1,806,072 - Pf6N2E2_1546 0.67 -0.6 1,806,072 - Pf6N2E2_1546 0.67 +0.6 1,806,072 - Pf6N2E2_1546 0.67 +0.9 1,806,239 + Pf6N2E2_1546 0.86 -0.6 1,806,240 - Pf6N2E2_1546 0.86 -0.2 1,806,240 - Pf6N2E2_1546 0.86 -0.5 1,806,323 + +0.8 1,806,324 - +0.3
Or see this region's nucleotide sequence