Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1482 and Pf6N2E2_1483 are separated by 192 nucleotides Pf6N2E2_1483 and Pf6N2E2_1484 are separated by 28 nucleotides Pf6N2E2_1484 and Pf6N2E2_1485 are separated by 113 nucleotides
Pf6N2E2_1482 - Probable periplasmic spermidine/putrescine-binding protein, at 1,731,787 to 1,732,890
_1482
Pf6N2E2_1483 - transcriptional regulator, LuxR family, at 1,733,083 to 1,733,820
_1483
Pf6N2E2_1484 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,733,849 to 1,734,778
_1484
Pf6N2E2_1485 - Transcriptional regulator, at 1,734,892 to 1,735,776
_1485
Position (kb)
1733
1734
1735 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1732.948 kb on + strand at 1732.951 kb on - strand at 1732.968 kb on - strand at 1732.970 kb on + strand at 1732.971 kb on - strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.985 kb on - strand at 1732.989 kb on - strand at 1733.015 kb on + strand at 1733.487 kb on - strand, within Pf6N2E2_1483 at 1733.700 kb on - strand, within Pf6N2E2_1483 at 1733.957 kb on - strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.259 kb on - strand, within Pf6N2E2_1484 at 1734.501 kb on + strand, within Pf6N2E2_1484 at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.113 kb on - strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.564 kb on + strand, within Pf6N2E2_1485 at 1735.565 kb on - strand, within Pf6N2E2_1485 at 1735.600 kb on + strand, within Pf6N2E2_1485
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,732,948 + +1.1 1,732,951 - +1.4 1,732,968 - -0.2 1,732,970 + +0.9 1,732,971 - +0.1 1,732,984 + -0.5 1,732,984 + -0.2 1,732,984 + +0.7 1,732,985 - +0.8 1,732,989 - +0.9 1,733,015 + +0.4 1,733,487 - Pf6N2E2_1483 0.55 -0.4 1,733,700 - Pf6N2E2_1483 0.84 -1.1 1,733,957 - Pf6N2E2_1484 0.12 +1.0 1,734,050 + Pf6N2E2_1484 0.22 +0.1 1,734,050 + Pf6N2E2_1484 0.22 +0.7 1,734,050 + Pf6N2E2_1484 0.22 +1.1 1,734,050 + Pf6N2E2_1484 0.22 +2.3 1,734,051 - Pf6N2E2_1484 0.22 +0.7 1,734,051 - Pf6N2E2_1484 0.22 -0.0 1,734,051 - Pf6N2E2_1484 0.22 +1.7 1,734,259 - Pf6N2E2_1484 0.44 +2.0 1,734,501 + Pf6N2E2_1484 0.70 +3.0 1,734,761 + +3.3 1,734,761 + +2.9 1,734,761 + +2.7 1,734,761 + +2.9 1,734,762 - +0.7 1,734,762 - -1.3 1,734,762 - -0.6 1,735,094 + Pf6N2E2_1485 0.23 -0.5 1,735,094 + Pf6N2E2_1485 0.23 -0.1 1,735,095 - Pf6N2E2_1485 0.23 -0.0 1,735,095 - Pf6N2E2_1485 0.23 +0.5 1,735,112 + Pf6N2E2_1485 0.25 -0.0 1,735,112 + Pf6N2E2_1485 0.25 +0.4 1,735,113 - Pf6N2E2_1485 0.25 -0.5 1,735,265 + Pf6N2E2_1485 0.42 -0.9 1,735,265 + Pf6N2E2_1485 0.42 -0.3 1,735,265 + Pf6N2E2_1485 0.42 +0.2 1,735,265 + Pf6N2E2_1485 0.42 +0.1 1,735,265 + +0.7 1,735,265 + Pf6N2E2_1485 0.42 +0.8 1,735,564 + Pf6N2E2_1485 0.76 +0.1 1,735,565 - Pf6N2E2_1485 0.76 -0.9 1,735,600 + Pf6N2E2_1485 0.80 +1.9
Or see this region's nucleotide sequence