Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotides Pf6N2E2_1433 and Pf6N2E2_1434 are separated by 13 nucleotides Pf6N2E2_1434 and Pf6N2E2_1435 are separated by 14 nucleotides
Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658
_1432
Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508
_1433
Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682
_1434
Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794
_1435
Position (kb)
1683
1684
1685 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1682.573 kb on + strand at 1682.606 kb on + strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.638 kb on + strand at 1682.638 kb on + strand at 1682.911 kb on + strand, within Pf6N2E2_1433 at 1683.031 kb on - strand, within Pf6N2E2_1433 at 1683.233 kb on - strand, within Pf6N2E2_1433 at 1683.334 kb on - strand, within Pf6N2E2_1433 at 1683.466 kb on + strand at 1683.466 kb on + strand at 1683.467 kb on - strand at 1683.841 kb on + strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1684.262 kb on - strand, within Pf6N2E2_1434 at 1684.581 kb on - strand at 1684.581 kb on - strand at 1684.581 kb on - strand at 1684.698 kb on + strand at 1684.777 kb on - strand at 1685.163 kb on - strand, within Pf6N2E2_1435 at 1685.185 kb on - strand, within Pf6N2E2_1435 at 1685.284 kb on - strand, within Pf6N2E2_1435 at 1685.649 kb on + strand, within Pf6N2E2_1435
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,682,573 + -0.2 1,682,606 + +0.4 1,682,607 - -0.2 1,682,607 - +0.5 1,682,607 - +0.3 1,682,607 - +0.6 1,682,607 - +0.6 1,682,607 - +0.0 1,682,638 + +0.6 1,682,638 + -0.3 1,682,911 + Pf6N2E2_1433 0.24 -1.7 1,683,031 - Pf6N2E2_1433 0.39 -0.4 1,683,233 - Pf6N2E2_1433 0.65 +0.3 1,683,334 - Pf6N2E2_1433 0.78 +0.7 1,683,466 + -0.4 1,683,466 + +0.4 1,683,467 - +0.7 1,683,841 + Pf6N2E2_1434 0.27 +2.3 1,683,860 - Pf6N2E2_1434 0.29 +2.8 1,683,860 - Pf6N2E2_1434 0.29 -1.9 1,684,262 - Pf6N2E2_1434 0.64 +0.8 1,684,581 - +0.3 1,684,581 - +2.0 1,684,581 - +0.9 1,684,698 + -0.3 1,684,777 - +0.2 1,685,163 - Pf6N2E2_1435 0.42 +0.1 1,685,185 - Pf6N2E2_1435 0.44 +0.6 1,685,284 - Pf6N2E2_1435 0.53 -1.9 1,685,649 + Pf6N2E2_1435 0.87 +1.0
Or see this region's nucleotide sequence