Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1338 and Pf6N2E2_1339 are separated by 181 nucleotides Pf6N2E2_1339 and Pf6N2E2_1340 overlap by 23 nucleotides Pf6N2E2_1340 and Pf6N2E2_1341 are separated by 10 nucleotides
Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016
_1338
Pf6N2E2_1339 - Acetylornithine deacetylase (EC 3.5.1.16), at 1,575,198 to 1,576,382
_1339
Pf6N2E2_1340 - major pilin protein fimA, at 1,576,360 to 1,577,034
_1340
Pf6N2E2_1341 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 1,577,045 to 1,577,431
_1341
Position (kb)
1575
1576
1577 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.520 kb on + strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.619 kb on + strand, within Pf6N2E2_1338 at 1574.620 kb on - strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.836 kb on - strand, within Pf6N2E2_1338 at 1574.887 kb on - strand, within Pf6N2E2_1338 at 1574.993 kb on - strand at 1575.082 kb on - strand at 1575.082 kb on - strand at 1575.085 kb on + strand at 1575.102 kb on - strand at 1575.223 kb on + strand at 1575.223 kb on + strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.280 kb on + strand at 1575.280 kb on + strand at 1575.281 kb on - strand at 1575.539 kb on - strand, within Pf6N2E2_1339 at 1576.231 kb on + strand, within Pf6N2E2_1339 at 1576.231 kb on + strand, within Pf6N2E2_1339 at 1576.267 kb on + strand at 1576.268 kb on - strand at 1576.268 kb on - strand at 1576.899 kb on + strand, within Pf6N2E2_1340 at 1576.900 kb on - strand, within Pf6N2E2_1340 at 1576.990 kb on + strand at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.349 kb on + strand at 1577.349 kb on + strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,574,469 + Pf6N2E2_1338 0.47 +0.5 1,574,469 + Pf6N2E2_1338 0.47 +0.5 1,574,470 - Pf6N2E2_1338 0.47 -1.9 1,574,470 - Pf6N2E2_1338 0.47 +0.0 1,574,470 - Pf6N2E2_1338 0.47 -0.7 1,574,470 - Pf6N2E2_1338 0.47 -0.4 1,574,520 + Pf6N2E2_1338 0.52 +0.9 1,574,521 - Pf6N2E2_1338 0.52 +0.2 1,574,521 - Pf6N2E2_1338 0.52 -0.4 1,574,619 + Pf6N2E2_1338 0.62 +0.9 1,574,620 - Pf6N2E2_1338 0.62 -0.8 1,574,643 + Pf6N2E2_1338 0.64 +0.8 1,574,643 + Pf6N2E2_1338 0.64 -0.1 1,574,643 + Pf6N2E2_1338 0.64 +1.0 1,574,643 + Pf6N2E2_1338 0.64 +0.2 1,574,644 - Pf6N2E2_1338 0.64 -0.4 1,574,644 - Pf6N2E2_1338 0.64 -2.3 1,574,682 + Pf6N2E2_1338 0.68 -0.3 1,574,682 + Pf6N2E2_1338 0.68 +0.4 1,574,683 - Pf6N2E2_1338 0.68 +0.3 1,574,683 - Pf6N2E2_1338 0.68 -1.1 1,574,683 - Pf6N2E2_1338 0.68 +1.7 1,574,683 - Pf6N2E2_1338 0.68 -0.7 1,574,683 - Pf6N2E2_1338 0.68 +0.6 1,574,835 + Pf6N2E2_1338 0.83 +0.3 1,574,835 + Pf6N2E2_1338 0.83 +0.7 1,574,836 - Pf6N2E2_1338 0.83 -1.7 1,574,887 - Pf6N2E2_1338 0.88 -0.7 1,574,993 - +0.3 1,575,082 - +0.8 1,575,082 - +0.2 1,575,085 + -1.1 1,575,102 - +0.4 1,575,223 + +1.3 1,575,223 + +0.3 1,575,224 - -2.1 1,575,224 - -0.5 1,575,224 - +0.2 1,575,280 + -0.5 1,575,280 + -0.2 1,575,281 - +0.2 1,575,539 - Pf6N2E2_1339 0.29 +0.6 1,576,231 + Pf6N2E2_1339 0.87 -0.7 1,576,231 + Pf6N2E2_1339 0.87 -0.8 1,576,267 + -2.1 1,576,268 - +0.7 1,576,268 - +0.7 1,576,899 + Pf6N2E2_1340 0.80 +0.2 1,576,900 - Pf6N2E2_1340 0.80 -0.2 1,576,990 + -0.5 1,577,199 + Pf6N2E2_1341 0.40 +3.3 1,577,199 + Pf6N2E2_1341 0.40 +0.7 1,577,199 + Pf6N2E2_1341 0.40 +0.3 1,577,199 + Pf6N2E2_1341 0.40 -3.1 1,577,200 - Pf6N2E2_1341 0.40 +3.1 1,577,200 - Pf6N2E2_1341 0.40 -0.2 1,577,200 - Pf6N2E2_1341 0.40 +0.5 1,577,349 + +0.7 1,577,349 + Pf6N2E2_1341 0.79 -2.4 1,577,350 - Pf6N2E2_1341 0.79 +0.9 1,577,350 - Pf6N2E2_1341 0.79 +0.7
Or see this region's nucleotide sequence