Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1312 and Pf6N2E2_1313 are separated by 12 nucleotides Pf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotides Pf6N2E2_1314 and Pf6N2E2_1315 are separated by 144 nucleotides Pf6N2E2_1315 and Pf6N2E2_1316 are separated by 252 nucleotides
Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267
_1312
Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083
_1313
Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895
_1314
Pf6N2E2_1315 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 1,549,040 to 1,549,462
_1315
Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656
_1316
Position (kb)
1548
1549 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1547.140 kb on - strand at 1547.190 kb on + strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.397 kb on + strand, within Pf6N2E2_1313 at 1547.398 kb on - strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.953 kb on - strand, within Pf6N2E2_1313 at 1548.182 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.678 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.771 kb on - strand, within Pf6N2E2_1314 at 1548.811 kb on + strand, within Pf6N2E2_1314 at 1548.891 kb on - strand at 1549.104 kb on - strand, within Pf6N2E2_1315 at 1549.125 kb on + strand, within Pf6N2E2_1315 at 1549.497 kb on + strand at 1549.508 kb on + strand at 1549.702 kb on + strand at 1549.703 kb on - strand at 1549.859 kb on + strand, within Pf6N2E2_1316
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,547,140 - +0.3 1,547,190 + -2.6 1,547,191 - +0.5 1,547,191 - +0.4 1,547,191 - -0.9 1,547,191 - -0.3 1,547,397 + Pf6N2E2_1313 0.15 +1.2 1,547,398 - Pf6N2E2_1313 0.15 -0.6 1,547,856 + Pf6N2E2_1313 0.72 -0.3 1,547,856 + Pf6N2E2_1313 0.72 -0.9 1,547,953 - Pf6N2E2_1313 0.84 +1.1 1,548,182 + Pf6N2E2_1314 0.11 -1.6 1,548,450 + Pf6N2E2_1314 0.44 -0.3 1,548,450 + Pf6N2E2_1314 0.44 +0.1 1,548,450 + Pf6N2E2_1314 0.44 +0.4 1,548,451 - Pf6N2E2_1314 0.44 -0.6 1,548,451 - Pf6N2E2_1314 0.44 -3.4 1,548,678 + Pf6N2E2_1314 0.73 -0.5 1,548,770 + Pf6N2E2_1314 0.84 -0.4 1,548,770 + Pf6N2E2_1314 0.84 +0.8 1,548,770 + Pf6N2E2_1314 0.84 -3.0 1,548,771 - Pf6N2E2_1314 0.84 -0.1 1,548,811 + Pf6N2E2_1314 0.89 -0.4 1,548,891 - +0.6 1,549,104 - Pf6N2E2_1315 0.15 +0.7 1,549,125 + Pf6N2E2_1315 0.20 -0.5 1,549,497 + -0.5 1,549,508 + -0.1 1,549,702 + -0.5 1,549,703 - +0.7 1,549,859 + Pf6N2E2_1316 0.15 -0.2
Or see this region's nucleotide sequence