Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1314

Experiment: Phosphomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1312 and Pf6N2E2_1313 are separated by 12 nucleotidesPf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotidesPf6N2E2_1314 and Pf6N2E2_1315 are separated by 144 nucleotidesPf6N2E2_1315 and Pf6N2E2_1316 are separated by 252 nucleotides Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267 _1312 Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083 _1313 Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895 _1314 Pf6N2E2_1315 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 1,549,040 to 1,549,462 _1315 Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656 _1316 Position (kb) 1548 1549Strain fitness (log2 ratio) -3 -2 -1 0 1at 1547.140 kb on - strandat 1547.190 kb on + strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.191 kb on - strandat 1547.397 kb on + strand, within Pf6N2E2_1313at 1547.398 kb on - strand, within Pf6N2E2_1313at 1547.856 kb on + strand, within Pf6N2E2_1313at 1547.856 kb on + strand, within Pf6N2E2_1313at 1547.953 kb on - strand, within Pf6N2E2_1313at 1548.182 kb on + strand, within Pf6N2E2_1314at 1548.450 kb on + strand, within Pf6N2E2_1314at 1548.450 kb on + strand, within Pf6N2E2_1314at 1548.450 kb on + strand, within Pf6N2E2_1314at 1548.451 kb on - strand, within Pf6N2E2_1314at 1548.451 kb on - strand, within Pf6N2E2_1314at 1548.678 kb on + strand, within Pf6N2E2_1314at 1548.770 kb on + strand, within Pf6N2E2_1314at 1548.770 kb on + strand, within Pf6N2E2_1314at 1548.770 kb on + strand, within Pf6N2E2_1314at 1548.771 kb on - strand, within Pf6N2E2_1314at 1548.811 kb on + strand, within Pf6N2E2_1314at 1548.891 kb on - strandat 1549.104 kb on - strand, within Pf6N2E2_1315at 1549.125 kb on + strand, within Pf6N2E2_1315at 1549.497 kb on + strandat 1549.508 kb on + strandat 1549.702 kb on + strandat 1549.703 kb on - strandat 1549.859 kb on + strand, within Pf6N2E2_1316

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml
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1,547,140 - +0.3
1,547,190 + -2.6
1,547,191 - +0.5
1,547,191 - +0.4
1,547,191 - -0.9
1,547,191 - -0.3
1,547,397 + Pf6N2E2_1313 0.15 +1.2
1,547,398 - Pf6N2E2_1313 0.15 -0.6
1,547,856 + Pf6N2E2_1313 0.72 -0.3
1,547,856 + Pf6N2E2_1313 0.72 -0.9
1,547,953 - Pf6N2E2_1313 0.84 +1.1
1,548,182 + Pf6N2E2_1314 0.11 -1.6
1,548,450 + Pf6N2E2_1314 0.44 -0.3
1,548,450 + Pf6N2E2_1314 0.44 +0.1
1,548,450 + Pf6N2E2_1314 0.44 +0.4
1,548,451 - Pf6N2E2_1314 0.44 -0.6
1,548,451 - Pf6N2E2_1314 0.44 -3.4
1,548,678 + Pf6N2E2_1314 0.73 -0.5
1,548,770 + Pf6N2E2_1314 0.84 -0.4
1,548,770 + Pf6N2E2_1314 0.84 +0.8
1,548,770 + Pf6N2E2_1314 0.84 -3.0
1,548,771 - Pf6N2E2_1314 0.84 -0.1
1,548,811 + Pf6N2E2_1314 0.89 -0.4
1,548,891 - +0.6
1,549,104 - Pf6N2E2_1315 0.15 +0.7
1,549,125 + Pf6N2E2_1315 0.20 -0.5
1,549,497 + -0.5
1,549,508 + -0.1
1,549,702 + -0.5
1,549,703 - +0.7
1,549,859 + Pf6N2E2_1316 0.15 -0.2

Or see this region's nucleotide sequence