Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1304 and Pf6N2E2_1305 are separated by 171 nucleotides Pf6N2E2_1305 and Pf6N2E2_1306 are separated by 83 nucleotides Pf6N2E2_1306 and Pf6N2E2_1307 are separated by 186 nucleotides Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides
Pf6N2E2_1304 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 1,539,474 to 1,539,722
_1304
Pf6N2E2_1305 - Homoprotocatechuate degradative operon repressor, at 1,539,894 to 1,540,295
_1305
Pf6N2E2_1306 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 1,540,379 to 1,541,284
_1306
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1539.389 kb on + strand at 1539.389 kb on + strand at 1539.980 kb on - strand, within Pf6N2E2_1305 at 1540.113 kb on + strand, within Pf6N2E2_1305 at 1540.114 kb on - strand, within Pf6N2E2_1305 at 1540.114 kb on - strand, within Pf6N2E2_1305 at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.351 kb on - strand at 1540.360 kb on - strand at 1540.662 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.749 kb on - strand, within Pf6N2E2_1306 at 1540.851 kb on + strand, within Pf6N2E2_1306 at 1541.079 kb on + strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.099 kb on + strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.124 kb on + strand, within Pf6N2E2_1306 at 1541.206 kb on - strand at 1541.206 kb on - strand at 1541.390 kb on + strand at 1541.407 kb on - strand at 1541.751 kb on - strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.912 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1542.058 kb on - strand, within Pf6N2E2_1307 at 1542.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,539,389 + -1.7 1,539,389 + +0.7 1,539,980 - Pf6N2E2_1305 0.21 -0.3 1,540,113 + Pf6N2E2_1305 0.54 +1.2 1,540,114 - Pf6N2E2_1305 0.55 -0.1 1,540,114 - Pf6N2E2_1305 0.55 +0.4 1,540,350 + -0.9 1,540,350 + +0.9 1,540,350 + -0.4 1,540,350 + -0.6 1,540,351 - +0.9 1,540,360 - +0.9 1,540,662 + Pf6N2E2_1306 0.31 -0.9 1,540,748 + Pf6N2E2_1306 0.41 -0.9 1,540,748 + Pf6N2E2_1306 0.41 +0.3 1,540,749 - Pf6N2E2_1306 0.41 +1.1 1,540,851 + Pf6N2E2_1306 0.52 +0.1 1,541,079 + Pf6N2E2_1306 0.77 +1.1 1,541,080 - Pf6N2E2_1306 0.77 +0.7 1,541,080 - Pf6N2E2_1306 0.77 -0.1 1,541,099 + Pf6N2E2_1306 0.79 -0.3 1,541,100 - Pf6N2E2_1306 0.80 +0.7 1,541,100 - Pf6N2E2_1306 0.80 -1.5 1,541,124 + Pf6N2E2_1306 0.82 +3.6 1,541,206 - -0.9 1,541,206 - +0.7 1,541,390 + -0.2 1,541,407 - +0.4 1,541,751 - Pf6N2E2_1307 0.41 +0.8 1,541,801 + Pf6N2E2_1307 0.49 +1.6 1,541,801 + Pf6N2E2_1307 0.49 -0.1 1,541,801 + Pf6N2E2_1307 0.49 -1.9 1,541,802 - Pf6N2E2_1307 0.49 -0.3 1,541,802 - Pf6N2E2_1307 0.49 -0.9 1,541,802 - Pf6N2E2_1307 0.49 -0.2 1,541,802 - Pf6N2E2_1307 0.49 +0.5 1,541,802 - Pf6N2E2_1307 0.49 +0.1 1,541,802 - Pf6N2E2_1307 0.49 -0.2 1,541,912 + Pf6N2E2_1307 0.65 +0.7 1,541,976 + Pf6N2E2_1307 0.74 -0.2 1,541,976 + Pf6N2E2_1307 0.74 +0.9 1,541,976 + Pf6N2E2_1307 0.74 -1.3 1,541,976 + Pf6N2E2_1307 0.74 +0.5 1,541,976 + Pf6N2E2_1307 0.74 -0.6 1,542,058 - Pf6N2E2_1307 0.87 -0.5 1,542,112 + -2.8
Or see this region's nucleotide sequence