Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1297 and Pf6N2E2_1298 are separated by 60 nucleotides Pf6N2E2_1298 and Pf6N2E2_1299 are separated by 141 nucleotides Pf6N2E2_1299 and Pf6N2E2_1300 are separated by 108 nucleotides
Pf6N2E2_1297 - Nucleoside-diphosphate-sugar epimerases, at 1,532,083 to 1,532,952
_1297
Pf6N2E2_1298 - Xanthine transporter,putative, at 1,533,013 to 1,534,242
_1298
Pf6N2E2_1299 - Transcriptional regulator, TetR family, at 1,534,384 to 1,535,019
_1299
Pf6N2E2_1300 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 1,535,128 to 1,535,919
_1300
Position (kb)
1533
1534
1535 Strain fitness (log2 ratio)
-2
-1
0
1 at 1532.025 kb on + strand at 1532.096 kb on + strand at 1532.944 kb on + strand at 1532.945 kb on - strand at 1532.953 kb on - strand at 1532.953 kb on - strand at 1532.997 kb on - strand at 1532.997 kb on - strand at 1533.023 kb on + strand at 1533.024 kb on - strand at 1533.171 kb on + strand, within Pf6N2E2_1298 at 1533.189 kb on - strand, within Pf6N2E2_1298 at 1533.189 kb on - strand, within Pf6N2E2_1298 at 1533.189 kb on - strand, within Pf6N2E2_1298 at 1533.300 kb on - strand, within Pf6N2E2_1298 at 1533.300 kb on - strand, within Pf6N2E2_1298 at 1533.491 kb on - strand, within Pf6N2E2_1298 at 1533.562 kb on + strand, within Pf6N2E2_1298 at 1533.582 kb on - strand, within Pf6N2E2_1298 at 1533.591 kb on + strand, within Pf6N2E2_1298 at 1533.744 kb on - strand, within Pf6N2E2_1298 at 1533.923 kb on + strand, within Pf6N2E2_1298 at 1534.144 kb on + strand at 1534.145 kb on - strand at 1534.145 kb on - strand at 1534.145 kb on - strand at 1534.227 kb on + strand at 1534.228 kb on - strand at 1534.228 kb on - strand at 1534.330 kb on - strand at 1534.562 kb on - strand, within Pf6N2E2_1299 at 1534.567 kb on + strand, within Pf6N2E2_1299 at 1534.568 kb on - strand, within Pf6N2E2_1299 at 1534.632 kb on + strand, within Pf6N2E2_1299 at 1534.633 kb on - strand at 1534.734 kb on - strand, within Pf6N2E2_1299 at 1535.078 kb on + strand at 1535.081 kb on + strand at 1535.081 kb on + strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,532,025 + +0.7 1,532,096 + -0.6 1,532,944 + -1.9 1,532,945 - +1.1 1,532,953 - -1.0 1,532,953 - -0.3 1,532,997 - +0.8 1,532,997 - -1.7 1,533,023 + +0.3 1,533,024 - -0.4 1,533,171 + Pf6N2E2_1298 0.13 +0.2 1,533,189 - Pf6N2E2_1298 0.14 +0.6 1,533,189 - Pf6N2E2_1298 0.14 +0.5 1,533,189 - Pf6N2E2_1298 0.14 +0.6 1,533,300 - Pf6N2E2_1298 0.23 -0.3 1,533,300 - Pf6N2E2_1298 0.23 +0.3 1,533,491 - Pf6N2E2_1298 0.39 -0.3 1,533,562 + Pf6N2E2_1298 0.45 +0.4 1,533,582 - Pf6N2E2_1298 0.46 +0.0 1,533,591 + Pf6N2E2_1298 0.47 -1.7 1,533,744 - Pf6N2E2_1298 0.59 -0.4 1,533,923 + Pf6N2E2_1298 0.74 +1.7 1,534,144 + -0.9 1,534,145 - -1.0 1,534,145 - +1.7 1,534,145 - +0.7 1,534,227 + +1.0 1,534,228 - -1.9 1,534,228 - +0.3 1,534,330 - -1.6 1,534,562 - Pf6N2E2_1299 0.28 +0.5 1,534,567 + Pf6N2E2_1299 0.29 -0.6 1,534,568 - Pf6N2E2_1299 0.29 -0.5 1,534,632 + Pf6N2E2_1299 0.39 +0.3 1,534,633 - -0.9 1,534,734 - Pf6N2E2_1299 0.55 +0.8 1,535,078 + -0.7 1,535,081 + +1.1 1,535,081 + +0.6 1,535,082 - -0.4 1,535,082 - -0.5 1,535,082 - +1.3 1,535,139 - -1.4
Or see this region's nucleotide sequence