Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1243 and Pf6N2E2_1244 are separated by 152 nucleotides Pf6N2E2_1244 and Pf6N2E2_1245 are separated by 121 nucleotides Pf6N2E2_1245 and Pf6N2E2_1246 are separated by 25 nucleotides Pf6N2E2_1246 and Pf6N2E2_1247 are separated by 88 nucleotides Pf6N2E2_1247 and Pf6N2E2_1248 are separated by 285 nucleotides
Pf6N2E2_1243 - FIG00953437: hypothetical protein, at 1,457,737 to 1,457,991
_1243
Pf6N2E2_1244 - hypothetical protein, at 1,458,144 to 1,458,374
_1244
Pf6N2E2_1245 - hypothetical protein, at 1,458,496 to 1,458,921
_1245
Pf6N2E2_1246 - FIG00961372: hypothetical protein, at 1,458,947 to 1,459,174
_1246
Pf6N2E2_1247 - hypothetical protein, at 1,459,263 to 1,459,448
_1247
Pf6N2E2_1248 - MutT/nudix family protein, at 1,459,734 to 1,460,096
_1248
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1458.069 kb on + strand at 1458.329 kb on - strand, within Pf6N2E2_1244 at 1458.409 kb on - strand at 1458.460 kb on + strand at 1458.515 kb on + strand at 1458.620 kb on - strand, within Pf6N2E2_1245 at 1458.625 kb on + strand, within Pf6N2E2_1245 at 1458.625 kb on + strand, within Pf6N2E2_1245 at 1458.625 kb on + strand, within Pf6N2E2_1245 at 1458.625 kb on + strand, within Pf6N2E2_1245 at 1458.625 kb on + strand, within Pf6N2E2_1245 at 1458.626 kb on - strand, within Pf6N2E2_1245 at 1458.626 kb on - strand, within Pf6N2E2_1245 at 1458.627 kb on + strand, within Pf6N2E2_1245 at 1458.627 kb on + strand, within Pf6N2E2_1245 at 1458.627 kb on + strand, within Pf6N2E2_1245 at 1458.627 kb on + strand, within Pf6N2E2_1245 at 1458.627 kb on + strand, within Pf6N2E2_1245 at 1458.628 kb on - strand, within Pf6N2E2_1245 at 1458.628 kb on - strand, within Pf6N2E2_1245 at 1458.628 kb on - strand, within Pf6N2E2_1245 at 1458.727 kb on + strand, within Pf6N2E2_1245 at 1458.728 kb on - strand, within Pf6N2E2_1245 at 1458.844 kb on + strand, within Pf6N2E2_1245 at 1458.845 kb on - strand, within Pf6N2E2_1245 at 1458.845 kb on - strand, within Pf6N2E2_1245 at 1458.847 kb on + strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.850 kb on + strand, within Pf6N2E2_1245 at 1459.025 kb on + strand, within Pf6N2E2_1246 at 1459.026 kb on - strand, within Pf6N2E2_1246 at 1459.026 kb on - strand, within Pf6N2E2_1246 at 1459.155 kb on + strand at 1459.155 kb on + strand at 1459.204 kb on - strand at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.363 kb on - strand, within Pf6N2E2_1247 at 1459.547 kb on - strand at 1459.547 kb on - strand at 1459.553 kb on - strand at 1459.593 kb on + strand at 1459.594 kb on - strand at 1459.594 kb on - strand at 1459.632 kb on + strand at 1459.652 kb on + strand at 1459.694 kb on + strand at 1459.694 kb on + strand at 1459.695 kb on - strand at 1459.695 kb on - strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.697 kb on - strand at 1459.706 kb on + strand at 1459.706 kb on + strand at 1459.859 kb on + strand, within Pf6N2E2_1248 at 1459.860 kb on - strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.124 kb on - strand at 1460.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,458,069 + -1.7 1,458,329 - Pf6N2E2_1244 0.80 +0.0 1,458,409 - +0.7 1,458,460 + +0.0 1,458,515 + +1.7 1,458,620 - Pf6N2E2_1245 0.29 +1.7 1,458,625 + Pf6N2E2_1245 0.30 -0.1 1,458,625 + Pf6N2E2_1245 0.30 +0.5 1,458,625 + Pf6N2E2_1245 0.30 +0.7 1,458,625 + Pf6N2E2_1245 0.30 -0.3 1,458,625 + Pf6N2E2_1245 0.30 -0.8 1,458,626 - Pf6N2E2_1245 0.31 -0.1 1,458,626 - Pf6N2E2_1245 0.31 +0.7 1,458,627 + Pf6N2E2_1245 0.31 -0.3 1,458,627 + Pf6N2E2_1245 0.31 +0.0 1,458,627 + Pf6N2E2_1245 0.31 -2.1 1,458,627 + Pf6N2E2_1245 0.31 -0.1 1,458,627 + Pf6N2E2_1245 0.31 -0.3 1,458,628 - Pf6N2E2_1245 0.31 +0.5 1,458,628 - Pf6N2E2_1245 0.31 +0.7 1,458,628 - Pf6N2E2_1245 0.31 -0.2 1,458,727 + Pf6N2E2_1245 0.54 +0.8 1,458,728 - Pf6N2E2_1245 0.54 +0.2 1,458,844 + Pf6N2E2_1245 0.82 +0.0 1,458,845 - Pf6N2E2_1245 0.82 -0.3 1,458,845 - Pf6N2E2_1245 0.82 +1.1 1,458,847 + Pf6N2E2_1245 0.82 +0.8 1,458,848 - Pf6N2E2_1245 0.83 +0.5 1,458,848 - Pf6N2E2_1245 0.83 -0.7 1,458,848 - Pf6N2E2_1245 0.83 -0.9 1,458,850 + Pf6N2E2_1245 0.83 +1.1 1,459,025 + Pf6N2E2_1246 0.34 -0.1 1,459,026 - Pf6N2E2_1246 0.35 -1.2 1,459,026 - Pf6N2E2_1246 0.35 -0.8 1,459,155 + -1.9 1,459,155 + +0.2 1,459,204 - +0.6 1,459,282 + Pf6N2E2_1247 0.10 +1.9 1,459,282 + Pf6N2E2_1247 0.10 +0.1 1,459,301 - Pf6N2E2_1247 0.20 +3.2 1,459,301 - Pf6N2E2_1247 0.20 -0.3 1,459,362 + Pf6N2E2_1247 0.53 -1.3 1,459,362 + Pf6N2E2_1247 0.53 -0.1 1,459,362 + Pf6N2E2_1247 0.53 +1.2 1,459,362 + Pf6N2E2_1247 0.53 -1.2 1,459,362 + Pf6N2E2_1247 0.53 +1.0 1,459,363 - Pf6N2E2_1247 0.54 -2.5 1,459,547 - +0.4 1,459,547 - +0.0 1,459,553 - -0.9 1,459,593 + +0.2 1,459,594 - -0.5 1,459,594 - +1.1 1,459,632 + +0.4 1,459,652 + -0.2 1,459,694 + +0.1 1,459,694 + +1.1 1,459,695 - +0.5 1,459,695 - -1.4 1,459,696 + +0.0 1,459,696 + -0.5 1,459,696 + +1.4 1,459,697 - +2.2 1,459,706 + +1.0 1,459,706 + +0.5 1,459,859 + Pf6N2E2_1248 0.34 +0.1 1,459,860 - Pf6N2E2_1248 0.35 +0.6 1,459,919 + Pf6N2E2_1248 0.51 +0.1 1,459,919 + Pf6N2E2_1248 0.51 -1.3 1,459,919 + +0.7 1,460,094 + -0.5 1,460,094 + -0.0 1,460,094 + -0.4 1,460,094 + -1.7 1,460,094 + -0.2 1,460,094 + +0.1 1,460,124 - +1.2 1,460,166 - -0.5
Or see this region's nucleotide sequence