Experiment: Phosphomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1078 and Pf6N2E2_1079 are separated by 561 nucleotides Pf6N2E2_1079 and Pf6N2E2_1080 are separated by 11 nucleotides
Pf6N2E2_1078 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 1,261,308 to 1,262,879
_1078
Pf6N2E2_1079 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,263,441 to 1,264,403
_1079
Pf6N2E2_1080 - hydrolase, alpha/beta fold family, at 1,264,415 to 1,265,446
_1080
Position (kb)
1263
1264
1265 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1262.503 kb on + strand, within Pf6N2E2_1078 at 1262.503 kb on + strand, within Pf6N2E2_1078 at 1262.503 kb on + strand at 1262.503 kb on + strand, within Pf6N2E2_1078 at 1262.503 kb on + strand, within Pf6N2E2_1078 at 1262.504 kb on - strand, within Pf6N2E2_1078 at 1262.504 kb on - strand, within Pf6N2E2_1078 at 1262.504 kb on - strand, within Pf6N2E2_1078 at 1262.504 kb on - strand, within Pf6N2E2_1078 at 1262.739 kb on + strand at 1262.740 kb on - strand at 1262.740 kb on - strand at 1262.879 kb on + strand at 1262.880 kb on - strand at 1262.880 kb on - strand at 1262.883 kb on + strand at 1262.883 kb on + strand at 1262.883 kb on + strand at 1262.883 kb on + strand at 1262.884 kb on - strand at 1263.036 kb on + strand at 1263.036 kb on + strand at 1263.068 kb on + strand at 1263.068 kb on + strand at 1263.157 kb on + strand at 1263.169 kb on + strand at 1263.169 kb on + strand at 1263.231 kb on + strand at 1263.248 kb on + strand at 1263.248 kb on + strand at 1263.289 kb on + strand at 1263.289 kb on + strand at 1263.381 kb on + strand at 1263.383 kb on + strand at 1263.515 kb on - strand at 1263.526 kb on + strand at 1263.544 kb on + strand, within Pf6N2E2_1079 at 1263.544 kb on + strand, within Pf6N2E2_1079 at 1263.544 kb on + strand, within Pf6N2E2_1079 at 1263.545 kb on - strand, within Pf6N2E2_1079 at 1263.567 kb on + strand, within Pf6N2E2_1079 at 1263.567 kb on + strand, within Pf6N2E2_1079 at 1263.567 kb on + strand, within Pf6N2E2_1079 at 1263.568 kb on - strand, within Pf6N2E2_1079 at 1263.621 kb on + strand, within Pf6N2E2_1079 at 1263.621 kb on + strand, within Pf6N2E2_1079 at 1263.621 kb on + strand, within Pf6N2E2_1079 at 1263.622 kb on - strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.835 kb on + strand, within Pf6N2E2_1079 at 1263.836 kb on - strand, within Pf6N2E2_1079 at 1263.836 kb on - strand, within Pf6N2E2_1079 at 1263.836 kb on - strand, within Pf6N2E2_1079 at 1264.093 kb on + strand, within Pf6N2E2_1079 at 1264.093 kb on + strand, within Pf6N2E2_1079 at 1264.093 kb on + strand, within Pf6N2E2_1079 at 1264.093 kb on + strand, within Pf6N2E2_1079 at 1264.094 kb on - strand, within Pf6N2E2_1079 at 1264.094 kb on - strand, within Pf6N2E2_1079 at 1264.094 kb on - strand, within Pf6N2E2_1079 at 1264.094 kb on - strand, within Pf6N2E2_1079 at 1264.094 kb on - strand, within Pf6N2E2_1079 at 1264.110 kb on + strand, within Pf6N2E2_1079 at 1264.110 kb on + strand, within Pf6N2E2_1079 at 1264.298 kb on - strand, within Pf6N2E2_1079 at 1264.380 kb on + strand at 1264.380 kb on + strand at 1264.389 kb on + strand at 1264.389 kb on + strand at 1264.390 kb on - strand at 1264.390 kb on - strand at 1264.554 kb on - strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.674 kb on + strand, within Pf6N2E2_1080 at 1264.675 kb on - strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.702 kb on - strand, within Pf6N2E2_1080 at 1264.893 kb on - strand at 1265.028 kb on - strand, within Pf6N2E2_1080 at 1265.089 kb on - strand, within Pf6N2E2_1080 at 1265.102 kb on - strand, within Pf6N2E2_1080 at 1265.263 kb on - strand, within Pf6N2E2_1080 at 1265.316 kb on + strand, within Pf6N2E2_1080 at 1265.317 kb on - strand, within Pf6N2E2_1080 at 1265.322 kb on + strand, within Pf6N2E2_1080 at 1265.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.004 mg/ml remove 1,262,503 + Pf6N2E2_1078 0.76 +0.6 1,262,503 + Pf6N2E2_1078 0.76 -0.3 1,262,503 + +0.1 1,262,503 + Pf6N2E2_1078 0.76 -0.1 1,262,503 + Pf6N2E2_1078 0.76 +0.7 1,262,504 - Pf6N2E2_1078 0.76 +0.7 1,262,504 - Pf6N2E2_1078 0.76 -1.2 1,262,504 - Pf6N2E2_1078 0.76 +0.3 1,262,504 - Pf6N2E2_1078 0.76 -0.4 1,262,739 + -0.1 1,262,740 - +1.0 1,262,740 - +0.2 1,262,879 + +1.2 1,262,880 - +0.1 1,262,880 - +1.2 1,262,883 + +1.6 1,262,883 + +0.4 1,262,883 + +0.8 1,262,883 + +0.7 1,262,884 - +0.2 1,263,036 + -1.5 1,263,036 + -0.0 1,263,068 + +0.2 1,263,068 + +0.3 1,263,157 + +0.5 1,263,169 + -0.3 1,263,169 + -0.5 1,263,231 + -0.9 1,263,248 + -0.1 1,263,248 + -0.1 1,263,289 + +0.3 1,263,289 + -0.5 1,263,381 + -0.6 1,263,383 + -0.3 1,263,515 - +0.3 1,263,526 + -0.2 1,263,544 + Pf6N2E2_1079 0.11 +0.2 1,263,544 + Pf6N2E2_1079 0.11 -1.6 1,263,544 + Pf6N2E2_1079 0.11 -1.5 1,263,545 - Pf6N2E2_1079 0.11 +0.8 1,263,567 + Pf6N2E2_1079 0.13 +0.4 1,263,567 + Pf6N2E2_1079 0.13 -0.3 1,263,567 + Pf6N2E2_1079 0.13 -0.9 1,263,568 - Pf6N2E2_1079 0.13 +2.0 1,263,621 + Pf6N2E2_1079 0.19 -0.0 1,263,621 + Pf6N2E2_1079 0.19 +0.5 1,263,621 + Pf6N2E2_1079 0.19 +1.1 1,263,622 - Pf6N2E2_1079 0.19 -0.1 1,263,835 + Pf6N2E2_1079 0.41 +0.9 1,263,835 + Pf6N2E2_1079 0.41 +0.1 1,263,835 + Pf6N2E2_1079 0.41 -0.9 1,263,835 + Pf6N2E2_1079 0.41 +0.2 1,263,835 + Pf6N2E2_1079 0.41 +0.6 1,263,835 + Pf6N2E2_1079 0.41 -0.7 1,263,835 + Pf6N2E2_1079 0.41 -0.2 1,263,835 + Pf6N2E2_1079 0.41 -0.6 1,263,836 - Pf6N2E2_1079 0.41 -1.0 1,263,836 - Pf6N2E2_1079 0.41 -0.5 1,263,836 - Pf6N2E2_1079 0.41 +1.1 1,264,093 + Pf6N2E2_1079 0.68 +0.3 1,264,093 + Pf6N2E2_1079 0.68 -0.5 1,264,093 + Pf6N2E2_1079 0.68 -1.8 1,264,093 + Pf6N2E2_1079 0.68 +0.0 1,264,094 - Pf6N2E2_1079 0.68 +0.3 1,264,094 - Pf6N2E2_1079 0.68 -0.9 1,264,094 - Pf6N2E2_1079 0.68 +0.1 1,264,094 - Pf6N2E2_1079 0.68 +0.8 1,264,094 - Pf6N2E2_1079 0.68 +0.9 1,264,110 + Pf6N2E2_1079 0.69 +0.9 1,264,110 + Pf6N2E2_1079 0.69 +0.4 1,264,298 - Pf6N2E2_1079 0.89 -0.3 1,264,380 + -0.2 1,264,380 + +0.6 1,264,389 + +2.0 1,264,389 + +0.5 1,264,390 - +0.7 1,264,390 - -0.0 1,264,554 - Pf6N2E2_1080 0.13 +1.2 1,264,560 + Pf6N2E2_1080 0.14 +0.3 1,264,560 + Pf6N2E2_1080 0.14 -0.9 1,264,674 + Pf6N2E2_1080 0.25 -1.2 1,264,675 - Pf6N2E2_1080 0.25 +1.0 1,264,701 + Pf6N2E2_1080 0.28 +2.6 1,264,701 + Pf6N2E2_1080 0.28 +0.2 1,264,701 + Pf6N2E2_1080 0.28 +0.1 1,264,702 - Pf6N2E2_1080 0.28 -0.4 1,264,893 - -0.3 1,265,028 - Pf6N2E2_1080 0.59 +1.1 1,265,089 - Pf6N2E2_1080 0.65 +0.3 1,265,102 - Pf6N2E2_1080 0.67 -0.7 1,265,263 - Pf6N2E2_1080 0.82 -0.1 1,265,316 + Pf6N2E2_1080 0.87 -0.0 1,265,317 - Pf6N2E2_1080 0.87 +1.6 1,265,322 + Pf6N2E2_1080 0.88 +1.2 1,265,350 - -1.5
Or see this region's nucleotide sequence