Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02320 and MMP_RS02325 overlap by 4 nucleotides MMP_RS02325 and MMP_RS02330 are separated by 93 nucleotides MMP_RS02330 and MMP_RS02335 are separated by 155 nucleotides MMP_RS02335 and MMP_RS02340 are separated by 47 nucleotides MMP_RS02340 and MMP_RS02345 are separated by 31 nucleotides
MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676
_RS02320
MMP_RS02325: MMP_RS02325 - epoxyqueuosine reductase, at 437,673 to 438,470
_RS02325
MMP_RS02330: MMP_RS02330 - dihydroorotate dehydrogenase, at 438,564 to 439,478
_RS02330
MMP_RS02335: MMP_RS02335 - DNA-directed RNA polymerase, at 439,634 to 440,197
_RS02335
MMP_RS02340: MMP_RS02340 - DNA-directed RNA polymerase subunit E'', at 440,245 to 440,421
_RS02340
MMP_RS02345: MMP_RS02345 - GTP-dependent dephospho-CoA kinase family protein, at 440,453 to 440,929
_RS02345
Position (kb) 438
439
440 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 437.586 kb on - strand at 437.587 kb on + strand at 437.627 kb on - strand at 437.667 kb on - strand at 437.674 kb on - strand at 437.726 kb on - strand at 437.914 kb on - strand, within MMP_RS02325 at 437.958 kb on + strand, within MMP_RS02325 at 438.000 kb on - strand, within MMP_RS02325 at 438.001 kb on + strand, within MMP_RS02325 at 438.001 kb on + strand, within MMP_RS02325 at 438.005 kb on - strand, within MMP_RS02325 at 438.005 kb on - strand, within MMP_RS02325 at 438.013 kb on + strand, within MMP_RS02325 at 438.069 kb on - strand, within MMP_RS02325 at 438.277 kb on + strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.331 kb on + strand, within MMP_RS02325 at 438.399 kb on + strand at 438.443 kb on - strand at 438.447 kb on + strand at 438.488 kb on + strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.532 kb on + strand at 438.848 kb on - strand at 439.271 kb on + strand at 439.508 kb on + strand at 439.509 kb on - strand at 439.530 kb on + strand at 439.534 kb on + strand at 439.548 kb on - strand at 439.549 kb on + strand at 439.549 kb on + strand at 439.550 kb on - strand at 439.550 kb on - strand at 439.550 kb on - strand at 439.550 kb on - strand at 439.551 kb on + strand at 439.551 kb on + strand at 439.551 kb on + strand at 439.551 kb on + strand at 439.552 kb on - strand at 439.582 kb on + strand at 439.638 kb on + strand at 439.858 kb on - strand at 439.862 kb on - strand at 439.862 kb on - strand at 439.863 kb on + strand at 440.058 kb on - strand at 440.059 kb on + strand at 440.066 kb on + strand at 440.080 kb on + strand at 440.181 kb on - strand at 440.210 kb on - strand at 440.216 kb on - strand at 440.337 kb on + strand, within MMP_RS02340 at 440.390 kb on - strand, within MMP_RS02340 at 440.391 kb on + strand, within MMP_RS02340 at 440.401 kb on - strand, within MMP_RS02340 at 440.415 kb on - strand at 440.420 kb on + strand at 440.439 kb on - strand at 440.441 kb on - strand at 440.441 kb on - strand at 440.457 kb on + strand at 440.460 kb on - strand at 440.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 437,586 - -0.9 437,587 + -0.1 437,627 - +0.2 437,667 - +0.3 437,674 - -0.2 437,726 - -0.1 437,914 - MMP_RS02325 0.30 +0.4 437,958 + MMP_RS02325 0.36 +0.2 438,000 - MMP_RS02325 0.41 +0.4 438,001 + MMP_RS02325 0.41 -0.5 438,001 + MMP_RS02325 0.41 -0.5 438,005 - MMP_RS02325 0.42 -0.2 438,005 - MMP_RS02325 0.42 +0.1 438,013 + MMP_RS02325 0.43 +0.7 438,069 - MMP_RS02325 0.50 -0.2 438,277 + MMP_RS02325 0.76 +0.4 438,330 - MMP_RS02325 0.82 +0.6 438,330 - MMP_RS02325 0.82 -0.2 438,330 - MMP_RS02325 0.82 +0.2 438,331 + MMP_RS02325 0.82 -0.0 438,399 + +0.6 438,443 - +0.0 438,447 + -0.0 438,488 + +0.2 438,493 - +0.2 438,493 - -0.9 438,493 - -0.1 438,493 - -0.0 438,532 + -4.5 438,848 - -1.7 439,271 + -1.0 439,508 + +0.0 439,509 - +0.7 439,530 + -0.8 439,534 + -1.4 439,548 - -4.5 439,549 + -0.8 439,549 + -1.3 439,550 - -2.9 439,550 - -3.7 439,550 - -2.0 439,550 - -3.2 439,551 + -2.3 439,551 + -2.4 439,551 + -1.9 439,551 + -1.9 439,552 - -2.7 439,582 + -0.7 439,638 + -2.7 439,858 - -1.7 439,862 - -2.9 439,862 - -0.2 439,863 + -2.4 440,058 - -3.8 440,059 + -3.5 440,066 + -4.1 440,080 + -3.4 440,181 - -3.5 440,210 - -2.6 440,216 - -3.6 440,337 + MMP_RS02340 0.52 -1.2 440,390 - MMP_RS02340 0.82 -2.9 440,391 + MMP_RS02340 0.82 -3.4 440,401 - MMP_RS02340 0.88 -3.5 440,415 - -2.1 440,420 + -1.1 440,439 - -3.3 440,441 - -2.4 440,441 - -3.0 440,457 + -3.8 440,460 - -3.0 440,475 - -2.5
Or see this region's nucleotide sequence