Experiment: Phosphomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1942 and Ac3H11_1943 overlap by 4 nucleotides Ac3H11_1943 and Ac3H11_1944 are separated by 187 nucleotides Ac3H11_1944 and Ac3H11_1945 are separated by 66 nucleotides Ac3H11_1945 and Ac3H11_1946 are separated by 128 nucleotides
Ac3H11_1942 - Phenylacetic acid degradation protein paaI, at 157,595 to 158,053
_1942
Ac3H11_1943 - Cu(I)-responsive transcriptional regulator, at 158,050 to 158,505
_1943
Ac3H11_1944 - COG1664: Integral membrane protein CcmA involved in cell shape determination, at 158,693 to 159,277
_1944
Ac3H11_1945 - Acyl-CoA hydrolase (EC 3.1.2.20), at 159,344 to 159,766
_1945
Ac3H11_1946 - Lipid A export ATP-binding/permease protein MsbA, at 159,895 to 161,742
_1946
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 157.860 kb on + strand, within Ac3H11_1942 at 157.861 kb on - strand, within Ac3H11_1942 at 157.861 kb on - strand, within Ac3H11_1942 at 157.946 kb on - strand, within Ac3H11_1942 at 158.330 kb on - strand, within Ac3H11_1943 at 158.448 kb on - strand, within Ac3H11_1943 at 158.497 kb on + strand at 158.497 kb on + strand at 158.517 kb on + strand at 158.517 kb on + strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.665 kb on + strand at 158.666 kb on - strand at 158.666 kb on - strand at 159.083 kb on + strand, within Ac3H11_1944 at 159.284 kb on + strand at 159.285 kb on - strand at 159.391 kb on + strand, within Ac3H11_1945 at 159.391 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.603 kb on + strand, within Ac3H11_1945 at 159.603 kb on + strand, within Ac3H11_1945 at 159.604 kb on - strand, within Ac3H11_1945 at 159.694 kb on + strand, within Ac3H11_1945 at 159.694 kb on + strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 160.024 kb on + strand at 160.024 kb on + strand at 160.025 kb on - strand at 160.025 kb on - strand at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.06 mg/ml remove 157,860 + Ac3H11_1942 0.58 +0.7 157,861 - Ac3H11_1942 0.58 -1.4 157,861 - Ac3H11_1942 0.58 -0.9 157,946 - Ac3H11_1942 0.76 +0.1 158,330 - Ac3H11_1943 0.61 +0.0 158,448 - Ac3H11_1943 0.87 -1.3 158,497 + -1.7 158,497 + -0.5 158,517 + -1.4 158,517 + +0.7 158,518 - -1.0 158,518 - -0.2 158,518 - -1.1 158,518 - +0.3 158,518 - -3.0 158,665 + -0.0 158,666 - -1.8 158,666 - -1.9 159,083 + Ac3H11_1944 0.67 -1.3 159,284 + -0.0 159,285 - -1.6 159,391 + Ac3H11_1945 0.11 +0.0 159,391 + Ac3H11_1945 0.11 -1.7 159,405 + Ac3H11_1945 0.14 -0.6 159,405 + Ac3H11_1945 0.14 -0.5 159,405 + Ac3H11_1945 0.14 +0.7 159,405 + Ac3H11_1945 0.14 -0.5 159,406 - Ac3H11_1945 0.15 +0.4 159,406 - Ac3H11_1945 0.15 +1.0 159,406 - Ac3H11_1945 0.15 +1.3 159,435 + Ac3H11_1945 0.22 -0.0 159,435 + Ac3H11_1945 0.22 +0.4 159,435 + Ac3H11_1945 0.22 +0.2 159,435 + Ac3H11_1945 0.22 +1.2 159,435 + Ac3H11_1945 0.22 -2.2 159,435 + Ac3H11_1945 0.22 -1.7 159,435 + Ac3H11_1945 0.22 -0.7 159,435 + Ac3H11_1945 0.22 -0.6 159,436 - Ac3H11_1945 0.22 -0.5 159,436 - Ac3H11_1945 0.22 -1.3 159,436 - Ac3H11_1945 0.22 -0.3 159,436 - Ac3H11_1945 0.22 +0.5 159,436 - Ac3H11_1945 0.22 -0.3 159,465 + Ac3H11_1945 0.29 +0.5 159,465 + Ac3H11_1945 0.29 -0.0 159,465 + Ac3H11_1945 0.29 +0.2 159,465 + Ac3H11_1945 0.29 -1.2 159,465 + Ac3H11_1945 0.29 -2.8 159,465 + Ac3H11_1945 0.29 +0.3 159,465 + Ac3H11_1945 0.29 -0.1 159,465 + Ac3H11_1945 0.29 -2.0 159,466 - Ac3H11_1945 0.29 -0.5 159,466 - Ac3H11_1945 0.29 -0.6 159,466 - Ac3H11_1945 0.29 -0.5 159,466 - Ac3H11_1945 0.29 -0.6 159,603 + Ac3H11_1945 0.61 -0.2 159,603 + Ac3H11_1945 0.61 -0.5 159,604 - Ac3H11_1945 0.61 -1.2 159,694 + Ac3H11_1945 0.83 -0.3 159,694 + Ac3H11_1945 0.83 -2.4 159,695 - Ac3H11_1945 0.83 -0.3 159,695 - Ac3H11_1945 0.83 -2.6 159,695 - Ac3H11_1945 0.83 -0.4 159,695 - Ac3H11_1945 0.83 -0.4 159,695 - Ac3H11_1945 0.83 -1.3 160,024 + +1.9 160,024 + +0.1 160,025 - +0.0 160,025 - +0.1 160,183 + Ac3H11_1946 0.16 +1.1 160,183 + Ac3H11_1946 0.16 +1.6 160,183 + Ac3H11_1946 0.16 +0.5 160,183 + Ac3H11_1946 0.16 +1.2 160,184 - Ac3H11_1946 0.16 +0.2 160,184 - Ac3H11_1946 0.16 +0.9 160,184 - Ac3H11_1946 0.16 +1.5
Or see this region's nucleotide sequence