Experiment: Phosphomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1003 and Ac3H11_1004 are separated by 305 nucleotides Ac3H11_1004 and Ac3H11_1005 are separated by 1 nucleotides Ac3H11_1005 and Ac3H11_1006 are separated by 108 nucleotides
Ac3H11_1003 - ABC transporter substrate-binding protein, at 127,223 to 128,461
_1003
Ac3H11_1004 - ABC transporter permease protein, at 128,767 to 129,681
_1004
Ac3H11_1005 - Putrescine transport system permease protein potI, at 129,683 to 130,570
_1005
Ac3H11_1006 - Transcriptional regulator, GntR family, at 130,679 to 131,344
_1006
Position (kb) 128
129
130 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 127.808 kb on - strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.865 kb on + strand, within Ac3H11_1003 at 127.865 kb on + strand, within Ac3H11_1003 at 128.090 kb on + strand, within Ac3H11_1003 at 128.156 kb on + strand, within Ac3H11_1003 at 128.157 kb on - strand, within Ac3H11_1003 at 128.157 kb on - strand, within Ac3H11_1003 at 128.198 kb on + strand, within Ac3H11_1003 at 128.199 kb on - strand, within Ac3H11_1003 at 128.199 kb on - strand, within Ac3H11_1003 at 128.219 kb on + strand, within Ac3H11_1003 at 128.220 kb on - strand, within Ac3H11_1003 at 128.220 kb on - strand, within Ac3H11_1003 at 128.273 kb on + strand, within Ac3H11_1003 at 128.366 kb on + strand at 128.423 kb on + strand at 128.423 kb on + strand at 128.423 kb on + strand at 128.423 kb on + strand at 128.423 kb on + strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.424 kb on - strand at 128.927 kb on - strand, within Ac3H11_1004 at 128.959 kb on + strand, within Ac3H11_1004 at 128.959 kb on + strand, within Ac3H11_1004 at 128.959 kb on + strand, within Ac3H11_1004 at 128.959 kb on + strand, within Ac3H11_1004 at 128.960 kb on - strand, within Ac3H11_1004 at 128.960 kb on - strand, within Ac3H11_1004 at 129.022 kb on + strand, within Ac3H11_1004 at 129.022 kb on + strand, within Ac3H11_1004 at 129.023 kb on - strand, within Ac3H11_1004 at 129.023 kb on - strand, within Ac3H11_1004 at 129.023 kb on - strand, within Ac3H11_1004 at 129.025 kb on + strand, within Ac3H11_1004 at 129.025 kb on + strand, within Ac3H11_1004 at 129.026 kb on - strand, within Ac3H11_1004 at 129.026 kb on - strand, within Ac3H11_1004 at 129.319 kb on + strand, within Ac3H11_1004 at 129.319 kb on + strand, within Ac3H11_1004 at 129.574 kb on + strand, within Ac3H11_1004 at 129.575 kb on - strand, within Ac3H11_1004 at 129.575 kb on - strand, within Ac3H11_1004 at 129.758 kb on + strand at 129.759 kb on - strand at 129.759 kb on - strand at 129.759 kb on - strand at 130.250 kb on + strand, within Ac3H11_1005 at 130.250 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.345 kb on + strand, within Ac3H11_1005 at 130.466 kb on + strand, within Ac3H11_1005 at 130.569 kb on - strand at 130.582 kb on + strand at 130.583 kb on - strand at 130.583 kb on - strand at 130.583 kb on - strand at 130.583 kb on - strand at 130.623 kb on + strand at 130.624 kb on - strand at 130.666 kb on + strand at 130.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.06 mg/ml remove 127,808 - Ac3H11_1003 0.47 -0.6 127,859 + Ac3H11_1003 0.51 +1.0 127,859 + Ac3H11_1003 0.51 -2.2 127,859 + Ac3H11_1003 0.51 +0.6 127,859 + Ac3H11_1003 0.51 +1.5 127,859 + Ac3H11_1003 0.51 -0.2 127,859 + Ac3H11_1003 0.51 -3.0 127,860 - Ac3H11_1003 0.51 +1.1 127,860 - Ac3H11_1003 0.51 +0.9 127,860 - Ac3H11_1003 0.51 +1.0 127,860 - Ac3H11_1003 0.51 -1.3 127,865 + Ac3H11_1003 0.52 -0.5 127,865 + Ac3H11_1003 0.52 +0.3 128,090 + Ac3H11_1003 0.70 -0.5 128,156 + Ac3H11_1003 0.75 +0.4 128,157 - Ac3H11_1003 0.75 -0.2 128,157 - Ac3H11_1003 0.75 -0.2 128,198 + Ac3H11_1003 0.79 -0.4 128,199 - Ac3H11_1003 0.79 -0.8 128,199 - Ac3H11_1003 0.79 -2.0 128,219 + Ac3H11_1003 0.80 -1.2 128,220 - Ac3H11_1003 0.80 -0.7 128,220 - Ac3H11_1003 0.80 -1.0 128,273 + Ac3H11_1003 0.85 +0.4 128,366 + -0.5 128,423 + -0.6 128,423 + +1.0 128,423 + +1.3 128,423 + +1.4 128,423 + -1.0 128,424 - +0.4 128,424 - -0.2 128,424 - -1.7 128,424 - +0.7 128,424 - -1.5 128,424 - -0.3 128,424 - -0.2 128,424 - -0.1 128,424 - +0.5 128,424 - +0.6 128,424 - +0.1 128,424 - -1.1 128,927 - Ac3H11_1004 0.17 +0.9 128,959 + Ac3H11_1004 0.21 -1.1 128,959 + Ac3H11_1004 0.21 -0.7 128,959 + Ac3H11_1004 0.21 +1.4 128,959 + Ac3H11_1004 0.21 +0.2 128,960 - Ac3H11_1004 0.21 +0.6 128,960 - Ac3H11_1004 0.21 +0.0 129,022 + Ac3H11_1004 0.28 +0.7 129,022 + Ac3H11_1004 0.28 +0.9 129,023 - Ac3H11_1004 0.28 -2.2 129,023 - Ac3H11_1004 0.28 +0.7 129,023 - Ac3H11_1004 0.28 +0.2 129,025 + Ac3H11_1004 0.28 +0.5 129,025 + Ac3H11_1004 0.28 -0.0 129,026 - Ac3H11_1004 0.28 +0.8 129,026 - Ac3H11_1004 0.28 +1.4 129,319 + Ac3H11_1004 0.60 +0.4 129,319 + Ac3H11_1004 0.60 +0.3 129,574 + Ac3H11_1004 0.88 +0.2 129,575 - Ac3H11_1004 0.88 -0.5 129,575 - Ac3H11_1004 0.88 +0.5 129,758 + +0.3 129,759 - -0.3 129,759 - +0.2 129,759 - +0.7 130,250 + Ac3H11_1005 0.64 -0.4 130,250 + Ac3H11_1005 0.64 +0.5 130,345 + Ac3H11_1005 0.75 +0.6 130,345 + Ac3H11_1005 0.75 +0.9 130,345 + Ac3H11_1005 0.75 +0.8 130,345 + Ac3H11_1005 0.75 +0.4 130,345 + Ac3H11_1005 0.75 +0.7 130,345 + Ac3H11_1005 0.75 +1.6 130,466 + Ac3H11_1005 0.88 +0.5 130,569 - -0.2 130,582 + -0.4 130,583 - +0.6 130,583 - +0.3 130,583 - -0.3 130,583 - -1.0 130,623 + +0.2 130,624 - +0.7 130,666 + +0.8 130,666 + +0.6
Or see this region's nucleotide sequence