Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2096 and Shew_2097 are separated by 422 nucleotides Shew_2097 and Shew_2098 are separated by 235 nucleotides Shew_2098 and Shew_2099 overlap by 27 nucleotides
Shew_2096: Shew_2096 - thiamine biosynthesis protein ThiC (RefSeq), at 2,422,909 to 2,424,960
_2096
Shew_2097: Shew_2097 - hypothetical protein (RefSeq), at 2,425,383 to 2,426,567
_2097
Shew_2098: Shew_2098 - hemolysin III family channel protein (RefSeq), at 2,426,803 to 2,427,513
_2098
Shew_2099: Shew_2099 - DTW domain-containing protein (RefSeq), at 2,427,487 to 2,428,056
_2099
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-1
0
1 at 2424.482 kb on - strand, within Shew_2096 at 2424.748 kb on + strand, within Shew_2096 at 2424.748 kb on + strand, within Shew_2096 at 2425.031 kb on + strand at 2425.241 kb on + strand at 2425.241 kb on + strand at 2425.241 kb on + strand at 2425.249 kb on - strand at 2425.249 kb on - strand at 2425.400 kb on + strand at 2426.077 kb on - strand, within Shew_2097 at 2426.796 kb on - strand at 2426.890 kb on + strand, within Shew_2098 at 2427.183 kb on - strand, within Shew_2098 at 2427.231 kb on + strand, within Shew_2098
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,424,482 - Shew_2096 0.77 -0.3 2,424,748 + Shew_2096 0.90 +0.9 2,424,748 + Shew_2096 0.90 +0.5 2,425,031 + -1.3 2,425,241 + +1.0 2,425,241 + +0.3 2,425,241 + -1.1 2,425,249 - +0.1 2,425,249 - +0.2 2,425,400 + +0.5 2,426,077 - Shew_2097 0.59 -0.1 2,426,796 - -0.6 2,426,890 + Shew_2098 0.12 +0.8 2,427,183 - Shew_2098 0.53 -1.7 2,427,231 + Shew_2098 0.60 +0.7
Or see this region's nucleotide sequence