Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2077 and Shew_2078 are separated by 7 nucleotides Shew_2078 and aspS are separated by 70 nucleotides aspS and Shew_2080 are separated by 169 nucleotides
Shew_2077: Shew_2077 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 2,401,257 to 2,401,778
_2077
Shew_2078: Shew_2078 - hypothetical protein (RefSeq), at 2,401,786 to 2,402,532
_2078
Shew_2079: aspS - aspartyl-tRNA synthetase (RefSeq), at 2,402,603 to 2,404,381
aspS
Shew_2080: Shew_2080 - diguanylate cyclase (RefSeq), at 2,404,551 to 2,407,355
_2080
Position (kb)
2402
2403
2404
2405 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2401.719 kb on - strand, within Shew_2077 at 2401.987 kb on - strand, within Shew_2078 at 2404.620 kb on - strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.763 kb on - strand at 2404.822 kb on + strand at 2404.855 kb on + strand, within Shew_2080 at 2405.016 kb on + strand, within Shew_2080 at 2405.059 kb on - strand, within Shew_2080 at 2405.059 kb on - strand, within Shew_2080 at 2405.174 kb on - strand, within Shew_2080 at 2405.236 kb on - strand, within Shew_2080
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,401,719 - Shew_2077 0.89 -4.5 2,401,987 - Shew_2078 0.27 -3.3 2,404,620 - +0.4 2,404,661 + -1.3 2,404,661 + +0.8 2,404,661 + +1.5 2,404,661 + -0.4 2,404,661 + -1.0 2,404,669 - +0.5 2,404,669 - -0.5 2,404,669 - +0.6 2,404,669 - +0.1 2,404,763 - -0.2 2,404,822 + -0.3 2,404,855 + Shew_2080 0.11 +0.0 2,405,016 + Shew_2080 0.17 +0.5 2,405,059 - Shew_2080 0.18 +0.1 2,405,059 - Shew_2080 0.18 -0.6 2,405,174 - Shew_2080 0.22 +1.9 2,405,236 - Shew_2080 0.24 +1.1
Or see this region's nucleotide sequence