Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1522 and Shew_1523 are separated by 158 nucleotides Shew_1523 and Shew_1524 overlap by 11 nucleotides Shew_1524 and Shew_1525 overlap by 1 nucleotides Shew_1525 and Shew_1526 are separated by 3 nucleotides
Shew_1522: Shew_1522 - prolyl oligopeptidase (RefSeq), at 1,745,372 to 1,747,462
_1522
Shew_1523: Shew_1523 - 4-hydroxybenzoyl-CoA thioesterase (RefSeq), at 1,747,621 to 1,748,019
_1523
Shew_1524: Shew_1524 - MotA/TolQ/ExbB proton channel (RefSeq), at 1,748,009 to 1,748,698
_1524
Shew_1525: Shew_1525 - biopolymer transport protein ExbD/TolR (RefSeq), at 1,748,698 to 1,749,132
_1525
Shew_1526: Shew_1526 - TolA family protein (RefSeq), at 1,749,136 to 1,750,071
_1526
Position (kb)
1748
1749 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1747.124 kb on + strand, within Shew_1522 at 1747.747 kb on - strand, within Shew_1523 at 1747.860 kb on + strand, within Shew_1523 at 1747.930 kb on - strand, within Shew_1523 at 1747.951 kb on + strand, within Shew_1523 at 1748.296 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.950 kb on + strand, within Shew_1525 at 1749.245 kb on - strand, within Shew_1526 at 1749.551 kb on - strand, within Shew_1526
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,747,124 + Shew_1522 0.84 -0.9 1,747,747 - Shew_1523 0.32 +0.5 1,747,860 + Shew_1523 0.60 +0.3 1,747,930 - Shew_1523 0.77 -2.8 1,747,951 + Shew_1523 0.83 -0.2 1,748,296 + Shew_1524 0.42 -1.4 1,748,437 + Shew_1524 0.62 -1.5 1,748,437 + Shew_1524 0.62 -2.1 1,748,950 + Shew_1525 0.58 -0.8 1,749,245 - Shew_1526 0.12 +0.2 1,749,551 - Shew_1526 0.44 -0.8
Or see this region's nucleotide sequence