Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and Shew_1368 overlap by 1 nucleotides Shew_1368 and fliI overlap by 29 nucleotides fliI and Shew_1370 are separated by 29 nucleotides Shew_1370 and Shew_1371 are separated by 380 nucleotides
Shew_1367: fliG - flagellar motor switch protein G (RefSeq), at 1,576,729 to 1,577,772
fliG
Shew_1368: Shew_1368 - flagellar assembly protein FliH (RefSeq), at 1,577,772 to 1,578,791
_1368
Shew_1369: fliI - flagellum-specific ATP synthase (RefSeq), at 1,578,763 to 1,580,103
fliI
Shew_1370: Shew_1370 - flagellar export protein FliJ (RefSeq), at 1,580,133 to 1,580,579
_1370
Shew_1371: Shew_1371 - flagellar hook-length control protein (RefSeq), at 1,580,960 to 1,582,666
_1371
Position (kb)
1578
1579
1580
1581 Strain fitness (log2 ratio)
-2
-1
0
1 at 1577.804 kb on + strand at 1577.956 kb on + strand, within Shew_1368 at 1577.981 kb on + strand, within Shew_1368 at 1578.043 kb on - strand, within Shew_1368 at 1578.061 kb on + strand, within Shew_1368 at 1578.565 kb on + strand, within Shew_1368 at 1578.565 kb on + strand, within Shew_1368 at 1578.920 kb on - strand, within fliI at 1579.284 kb on + strand, within fliI at 1579.292 kb on - strand, within fliI at 1579.866 kb on - strand, within fliI at 1580.254 kb on + strand, within Shew_1370 at 1580.598 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,577,804 + -0.5 1,577,956 + Shew_1368 0.18 -0.2 1,577,981 + Shew_1368 0.20 -0.9 1,578,043 - Shew_1368 0.27 -0.5 1,578,061 + Shew_1368 0.28 +0.2 1,578,565 + Shew_1368 0.78 -0.5 1,578,565 + Shew_1368 0.78 +0.0 1,578,920 - fliI Shew_1369 0.12 -0.2 1,579,284 + fliI Shew_1369 0.39 -0.2 1,579,292 - fliI Shew_1369 0.39 -1.9 1,579,866 - fliI Shew_1369 0.82 -0.6 1,580,254 + Shew_1370 0.27 -0.5 1,580,598 + +0.1
Or see this region's nucleotide sequence